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Molecule Parameter List for GDP.Ran-cytoplasmic

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*GDP.Ran-cytoplasmic* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	2	1	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
RanGTPase	70	Network	Shared_Object_RanGTPase, Nucleus, Cytoplasm
This model represents a concentration gradient of RanGTP across the nuclear envelope. This gradient is generated by distribution of regulators of RanGTPase. We have taken a log linear plot of graphs generated by GENESIS and compared with the experimental graphs.

GDP.Ran-cytoplasmic acting as a Molecule in RanGTPase Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
GDP.Ran-cytoplasmic	RanGTPase Accession No. : 70	Cytoplasm Pathway No. : 306	5	18000	No
cytoplasmic-GDP.Ran Cytoplasmic fraction of GDP bound Ran Cytoplasmic volume = 1.8 pL = 1.8e-15 m^3 Initial cytoplasmic GDP.Ran concentration = 5 uM Table II footnote Gorlich D et al (2003) EMBO J 22(5):1088-1100

GDP.Ran-cytoplasmic acting as a Product of an Enzyme in RanGTPase Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	RanGAP / RanGAP	RanGTPase Accession No. : 70	Cytoplasm Pathway No. : 306	0.1	10.8	4	explicit E-S complex	Substrate RanBP1.RanGTP Product GDP.Ran-cytoplas mic
	RanGAP reaction on RanGTP-RanBP1 complex kcat = 10.8 /sec Km = 0.1 uM at 25^oC Table 1. Gorlich D et al (2003) EMBO J 22(5):1088-1100
2	RanGAP / RanGAP[1]	RanGTPase Accession No. : 70	Cytoplasm Pathway No. : 306	0.699998	10.6	4	explicit E-S complex	Substrate GTP.Ran-cytoplas mic Product GDP.Ran-cytoplas mic
	RanGAP reaction on free RanGTP kcat = 10.6 /sec Km = 0.7 uM at 25^oC Table 1. Gorlich D et al (2003) EMBO J 22(5):1088-1100

GDP.Ran-cytoplasmic acting as a Substrate in a reaction in RanGTPase Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
NTF2_binding	RanGTPase Accession No. : 70	Cytoplasm Pathway No. : 306	1 (uM^-1 s^-1)	0.1 (s^-1)	Kd(bf) = 0.1(uM)	-	Substrate GDP.Ran-cytoplas mic NTF2 Product NTF2-GDP.Ran
Binding of NTF2 to cytoplasmic fraction of GDP.Ran Kd = 100 nM Catherine Chaillan-Huntington et al (2000) J.Biol.Chem. 275(8):5874-5879. call /kinetics/Cytoplasm/RanGTP_binding/notes LOAD Binding of cytoplasmic fraction of RanGTP to RanBP1 Kf = 3.0e+05 /M/sec = 0.3 /uM/sec Kb = 4.0e-04 /sec Carolina I. Villa Braslavsky et al (2000) Biochemistry 39(38):11629-11639. Supplementary Table A Gorlich D et al (2003) EMBO J 22(5):1088-1100

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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