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Molecule Parameter List for GDP.Ran-cytoplasmic | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by  color. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Statistics | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| GDP.Ran-cytoplasmic participated as | Molecule | Sum total of | Enzyme | Substrate of an enzyme | Product of an enzyme | Substrate in Reaction | Product in Reaction |
| No. of occurrences | 1 | 0 | 0 | 0 | 2 | 1 | 0 |
Accession and Pathway Details |
| Accession Name | Accession No. | Accession Type | Pathway Link |
| RanGTPase | 70 | Network | Shared_Object_RanGTPase, Nucleus, Cytoplasm |
| This model represents a concentration gradient of RanGTP across the nuclear envelope. This gradient is generated by distribution of regulators of RanGTPase. We have taken a log linear plot of graphs generated by GENESIS and compared with the experimental graphs. | |||
GDP.Ran-cytoplasmic acting as a Molecule in RanGTPase Network
| Name | Accession Name | Pathway Name | Initial Conc. (uM) | Volume (fL) | Buffered | |
| GDP.Ran-cytoplasmic | RanGTPase Accession No. : 70 | Cytoplasm Pathway No. : 306 | 5 | 18000 | No | |
| cytoplasmic-GDP.Ran Cytoplasmic fraction of GDP bound Ran Cytoplasmic volume = 1.8 pL = 1.8e-15 m^3 Initial cytoplasmic GDP.Ran concentration = 5 uM Table II footnote Gorlich D et al (2003) EMBO J 22(5):1088-1100 | ||||||
GDP.Ran-cytoplasmic acting as a Product of an Enzyme in RanGTPase Network
| Enzyme Molecule / Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | |
| 1 | RanGAP / RanGAP | RanGTPase Accession No. : 70 | Cytoplasm Pathway No. : 306 | 0.1 | 10.8 | 4 | explicit E-S complex | Substrate RanBP1.RanGTP Product mic |
| RanGAP reaction on RanGTP-RanBP1 complex kcat = 10.8 /sec Km = 0.1 uM at 25oC Table 1. Gorlich D et al (2003) EMBO J 22(5):1088-1100 | ||||||||
| 2 | RanGAP / RanGAP[1] | RanGTPase Accession No. : 70 | Cytoplasm Pathway No. : 306 | 0.699998 | 10.6 | 4 | explicit E-S complex | Substrate mic Product mic |
| RanGAP reaction on free RanGTP kcat = 10.6 /sec Km = 0.7 uM at 25oC Table 1. Gorlich D et al (2003) EMBO J 22(5):1088-1100 | ||||||||
GDP.Ran-cytoplasmic acting as a Substrate in a reaction in RanGTPase Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents |
| NTF2_binding | RanGTPase Accession No. : 70 | Cytoplasm Pathway No. : 306 | 1 (uM^-1 s^-1) | 0.1 (s^-1) | Kd(bf) = 0.1(uM) | - | Substrate mic NTF2 Product NTF2-GDP.Ran |
| Binding of NTF2 to cytoplasmic fraction of GDP.Ran Kd = 100 nM Catherine Chaillan-Huntington et al (2000) J.Biol.Chem. 275(8):5874-5879. call /kinetics/Cytoplasm/RanGTP_binding/notes LOAD Binding of cytoplasmic fraction of RanGTP to RanBP1 Kf = 3.0e+05 /M/sec = 0.3 /uM/sec Kb = 4.0e-04 /sec Carolina I. Villa Braslavsky et al (2000) Biochemistry 39(38):11629-11639. Supplementary Table A Gorlich D et al (2003) EMBO J 22(5):1088-1100 | |||||||