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Molecule Parameter List for NTF2

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
NTF2 participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000012

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
RanGTPase70Network
Shared_Object_RanGTPase,  Nucleus,  Cytoplasm 
This model represents a concentration gradient of RanGTP across the nuclear envelope. This gradient is generated by distribution of regulators of RanGTPase. We have taken a log linear plot of graphs generated by GENESIS and compared with the experimental graphs.

NTF2 acting as a Molecule in  RanGTPase Network
NameAccession NamePathway NameInitial Conc.
(uM)		Volume
(fL)		Buffered
NTF2RanGTPase
Accession No. : 70		Cytoplasm
Pathway No. : 306		0.318000Yes
NTF2: Nuclear Transport Factor 2 dimers Cytoplasmic volume = 1.8 pL = 1.8e-15 m^3 Total cellular concentration of NTF2 dimers = 0.3 uM Table II footnote Gorlich D et al (2003) EMBO J 22(5):1088-1100 Since it is the total cellular conc. the pool is buffered

NTF2 acting as a Substrate in a reaction in  RanGTPase Network
Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
NameAccession NamePathway NameKfKbKdtauReagents
NTF2_bindingRanGTPase
Accession No. : 70		Cytoplasm
Pathway No. : 306		1
(uM^-1 s^-1)		0.1
(s^-1)		Kd(bf) = 0.1(uM)-Substrate
  • GDP.Ran-cytoplas
    mic
    NTF2

    Product
    NTF2-GDP.Ran
    • Binding of NTF2 to cytoplasmic fraction of GDP.Ran Kd = 100 nM Catherine Chaillan-Huntington et al (2000) J.Biol.Chem. 275(8):5874-5879. call /kinetics/Cytoplasm/RanGTP_binding/notes LOAD Binding of cytoplasmic fraction of RanGTP to RanBP1 Kf = 3.0e+05 /M/sec = 0.3 /uM/sec Kb = 4.0e-04 /sec Carolina I. Villa Braslavsky et al (2000) Biochemistry 39(38):11629-11639. Supplementary Table A Gorlich D et al (2003) EMBO J 22(5):1088-1100

    NTF2 acting as a Product in a reaction in      RanGTPase Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1
  • NTF2_
    dissociation[1]
    		• RanGTPase
    Accession No. : 70    		Nucleus
    Pathway No. : 305    		0.48
    (s^-1)    		0
    (#^-1 s^-1)    		Not applicable**-Substrate
  • nuclear-NTF2-GDP
    .Ran

    Product
    GDP.Ran-nuclear
    NTF2
    •   Approximation of reaction rates to match the kinetics described by Gorlich D et al (2003) EMBO J 22(5):1088-1100
    2
  • NTF2_
    dissociation
    		• RanGTPase
    Accession No. : 70    		Cytoplasm
    Pathway No. : 306    		0.48
    (s^-1)    		0
    (#^-1 s^-1)    		Not applicable**-Substrate
    NTF2-GDP.Ran

    Product
    GDP.Ran-nuclear
    NTF2
      Approximation of rates to match the kinetics described by Gorlich D et al (2003) EMBO J 22(5):1088-1100
    ** This is a trasport reation between compartments of different volumes. Therefore Kd is not applicable. Please Note Kf, Kb units are in number of molecules instead of concentration


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