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Molecule Parameter List for Rec-Glu

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

Rec-Glu participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	0	1	2

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Ajay_Bhalla_ 2004_PKM_Tuning	76	Network	PKC,  Shared_Object_Ajay_Bhalla_2004_PKM_tuning,  PLA2,   PLCbeta,  Gq,  MAPK,   Ras,  EGFR,  Sos,   PLC_g,  CaMKII,  CaM,   PP1,  PP2B,  PKA,   AC,  PKM
This model is taken from the Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80. This is the reference feedforward model from Figure 8a.

Rec-Glu acting as a Molecule in  Ajay_Bhalla_2004_PKM_Tuning Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
Rec-Glu	Ajay_Bhalla_ 2004_PKM_Tuning Accession No. : 76	Gq Pathway No. : 316	0	1.5	No
This acts like an enzyme to activate the g proteins Assume cell has vol 1e-15 m^3 (10 uM cube), conversion factor to conc in uM is 6e5

Rec-Glu acting as a Substrate in a reaction in  Ajay_Bhalla_2004_PKM_Tuning Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
Rec-Glu-bind-Gq	Ajay_Bhalla_ 2004_PKM_Tuning Accession No. : 76	Gq Pathway No. : 316	0.006 (uM^-1 s^-1)	0.0001 (s^-1)	Kd(bf) = 0.0167(uM)	-	Substrate G-GDP Rec-Glu Product Rec-Glu-Gq
This is the k1-k2 equivalent for enzyme complex formation in the binding of Rec-Glu to Gq. See Fay et al Biochem 30 5066-5075 1991. kf = 5e-5 which is nearly the same as calculated by Fay et al. (4.67e-5) kb = .04 June 1996: Closer reading of Fay et al suggests that kb <= 0.0001, so kf = 1e-8 by detailed balance. This reaction appears to be neglible.

Rec-Glu acting as a Product in a reaction in  Ajay_Bhalla_2004_PKM_Tuning Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	RecLigandBinding	Ajay_Bhalla_ 2004_PKM_Tuning Accession No. : 76	Gq Pathway No. : 316	16.8003 (uM^-1 s^-1)	10 (s^-1)	Kd(bf) = 0.5952(uM)	-	Substrate Glu mGluR Product Rec-Glu
	kf = kf from text = 1e7 / M / sec = 10 /uM/sec = 10 / 6e5 / # / sec = 1.67e-5 kb = kr from text = 60 / sec Note that we continue to use uM here since [phenylephrine] is also in uM. From Martin et al FEBS Lett 316:2 191-196 1993 we have Kd = 600 nM Assuming kb = 10/sec, we get kf = 10/(0.6 uM * 6e5) = 2.8e-5 1/sec/#
2	Activate-Gq	Ajay_Bhalla_ 2004_PKM_Tuning Accession No. : 76	Gq Pathway No. : 316	0.01 (s^-1)	0 (uM^-2 s^-1)	-	-	Substrate Rec-Glu-Gq Product BetaGamma G*GTP Rec-Glu
	This is the kcat==k3 stage of the Rec-Glu ezymatic activation of Gq. From Berstein et al actiation is at .35 - 0.7/min From Fay et al Biochem 30 5066-5075 1991 kf = .01/sec From Nakamura et al J physiol Lond 474:1 35-41 1994 see time courses. Also (Berstein) 15-40% of gprot is in GTP-bound form on stim.