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Molecule Parameter List for MAPKK-ser | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | Ajay_Bhalla_
2004_PKM_Tuning | 76 | Network |
PKC, Shared_Object_Ajay_Bhalla_2004_PKM_tuning, PLA2,
PLCbeta, Gq, MAPK,
Ras, EGFR, Sos,
PLC_g, CaMKII, CaM,
PP1, PP2B, PKA,
AC, PKM | | This model is taken from the Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80. This is the reference feedforward model from Figure 8a. |
MAPKK-ser acting as a Molecule in Ajay_Bhalla_2004_PKM_Tuning Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | MAPKK-ser | Ajay_Bhalla_
2004_PKM_Tuning
Accession No. : 76 | MAPK
Pathway No. : 317 | 0 | 1.5 | No | | Intermediately phophorylated, assumed inactive, form of MAPKK |
MAPKK-ser acting as a Substrate for an Enzyme in Ajay_Bhalla_2004_PKM_Tuning Network
| | Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | | 1 | Raf-GTP-Ras /
Raf-GTP-Ras.2 | Ajay_Bhalla_
2004_PKM_Tuning
Accession No. : 76 | MAPK
Pathway No. : 317 | 0.159094 | 0.3 | 4 | explicit E-S complex | Substrate
MAPKK-ser
Product
MAPKK*
| | | Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591). | | 2 | Raf*-GTP-Ras /
Raf*-GTP-Ras.2 | Ajay_Bhalla_
2004_PKM_Tuning
Accession No. : 76 | MAPK
Pathway No. : 317 | 0.159094 | 0.3 | 4 | explicit E-S complex | Substrate
MAPKK-ser
Product
MAPKK*
| | | Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6 | | 3 | PPhosphatase2A /
MAPKK-deph-ser | Ajay_Bhalla_
2004_PKM_Tuning
Accession No. : 76 | Shared_Object_
Ajay_Bhalla_
2004_PKM_tuning
Pathway No. : 312 | 15.6568 | 6 | 4 | explicit E-S complex | Substrate
MAPKK-ser
Product
MAPKK
| | | |
MAPKK-ser acting as a Product of an Enzyme in Ajay_Bhalla_2004_PKM_Tuning Network
| | Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | | 1 | Raf-GTP-Ras /
Raf-GTP-Ras.1 | Ajay_Bhalla_
2004_PKM_Tuning
Accession No. : 76 | MAPK
Pathway No. : 317 | 0.159094 | 0.3 | 4 | explicit E-S complex | Substrate
MAPKK
Product
MAPKK-ser
| | | Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591). | | 2 | Raf*-GTP-Ras /
Raf*-GTP-Ras.1 | Ajay_Bhalla_
2004_PKM_Tuning
Accession No. : 76 | MAPK
Pathway No. : 317 | 0.159094 | 0.3 | 4 | explicit E-S complex | Substrate
MAPKK
Product
MAPKK-ser
| | | Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6 | | 3 | PPhosphatase2A /
MAPKK-deph | Ajay_Bhalla_
2004_PKM_Tuning
Accession No. : 76 | Shared_Object_
Ajay_Bhalla_
2004_PKM_tuning
Pathway No. : 312 | 15.6568 | 6 | 4 | explicit E-S complex | Substrate
MAPKK*
Product
MAPKK-ser
| | | See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms. |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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