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Molecule Parameter List for Ca.PLC_g | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | Ajay_Bhalla_
2004_PKM_MKP3_
Tuning | 77 | Network |
Shared_Object_Ajay_Bhalla_2004_PKM_MKP3_Tuning, PKC, PLA2,
PLCbeta, Ras, Gq,
MAPK, EGFR, Sos,
PLC_g, CaMKII, CaM,
PP1, PP2B, PKA,
AC, MKP3, PKM | | This model is based on Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80. This is the feedforward model with MPK3 from figure 8a. |
Ca.PLC_g acting as a Molecule in Ajay_Bhalla_2004_PKM_MKP3_Tuning Network
Ca.PLC_g acting as an Enzyme in Ajay_Bhalla_2004_PKM_MKP3_Tuning Network
Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | Ca.PLC_g /
PIP2_hydrolysis
| Ajay_Bhalla_
2004_PKM_MKP3_
Tuning
Accession No. : 77 | PLC_g
Pathway No. : 338 | 97.2222 | 14 | 4 | explicit E-S complex | Substrate
PIP2
Product
DAG
IP3
| | Mainly Homma et al JBC 263:14 1988 pp 6592, but these parms are the Ca-stimulated form. It is not clear whether the enzyme is activated by tyrosine phosphorylation at this point or not. Wahl et al JBC 267:15 10447-10456 1992 say that the Ca_stim and phosph form has 7X higher affinity for substrate than control. This is close to Wahl's figure 7, which I am using as reference. |
Ca.PLC_g acting as a Substrate for an Enzyme in Ajay_Bhalla_2004_PKM_MKP3_Tuning Network
Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | L.EGFR /
phosph_PLC_g | Ajay_Bhalla_
2004_PKM_MKP3_
Tuning
Accession No. : 77 | EGFR
Pathway No. : 336 | 0.333337 | 0.2 | 4 | explicit E-S complex | Substrate
Ca.PLC_g
Product
Ca.PLC_g*
| | Hsu et al JBC 266:1 603-608 1991 Km = 385 +- 100 uM, Vm = 5.1 +-1 pmol/min/ug for PLC-771. Other sites have similar range, but are not stim as much by EGF. k1 = 2.8e-2/385/6e5 = 1.2e-10. Phenomenally slow. But Sherrill and Kyte say turnover # for angiotensin II is 5/min for cell extt, and 2/min for placental. Also see Okada et al for Shc rates which are much faster. |
Ca.PLC_g acting as a Product in a reaction in Ajay_Bhalla_2004_PKM_MKP3_Tuning Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| | Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | 1 | Ca_act_PLC_g | Ajay_Bhalla_
2004_PKM_MKP3_
Tuning
Accession No. : 77 | PLC_g
Pathway No. : 338 | 180
(uM^-1 s^-1) | 10
(s^-1) | Kd(bf) = 0.0556(uM) | - | Substrate
Ca
PLC_g
Product
Ca.PLC_g
| | | Nice curves from Homma et al JBC 263:14 6592-6598 1988 Fig 5c. The activity falls above 10 uM, but that is too high to reach physiologically anyway, so we'll ignore the higher pts and match the lower ones only. Half-max at 1 uM. But Wahl et al JBC 267:15 10447-10456 1992 have half-max at 56 nM which is what I'll use. | | 2 | dephosph_PLC_g | Ajay_Bhalla_
2004_PKM_MKP3_
Tuning
Accession No. : 77 | PLC_g
Pathway No. : 338 | 0.05
(s^-1) | 0
(s^-1) | - | - | Substrate
Ca.PLC_g*
Product
Ca.PLC_g
|
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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