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Molecule Parameter List for MKP-3

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*MKP-3* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	2	0	1	1	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning	77	Network	Shared_Object_Ajay_Bhalla_2004_PKM_MKP3_Tuning, PKC, PLA2, PLCbeta, Ras, Gq, MAPK, EGFR, Sos, PLC_g, CaMKII, CaM, PP1, PP2B, PKA, AC, MKP3, PKM
This model is based on Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80. This is the feedforward model with MPK3 from figure 8a.

MKP-3 acting as a Molecule in Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
MKP-3	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	MKP3 Pathway No. : 345	0.02	1.5	No
MKP-3 dephosphoryates and inactivates MAPK in CA1 with a delay of under 30 min

MKP-3 acting as an Enzyme in Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	MKP-3 / MKP3-thr-deph	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	MKP3 Pathway No. : 345	10.0001	4	4	explicit E-S complex	Substrate MAPK* Product MAPK-tyr
	See MKP1-tyr-deph Km is twice as high to avoid saturation.
2	MKP-3 / MKP3-tyr-deph	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	MKP3 Pathway No. : 345	10.0001	4	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK
	The original kinetics have been modified to obey the k2 = 4 * k3 rule, while keeping kcat and Km fixed. As noted in the NOTES, the only constraining data point is the time course of MAPK dephosphorylation, which this model satisfies. It would be nice to have more accurate estimates of rate consts and MKP-1 levels from the literature. Effective Km : 67 nM kcat = 1.43 umol/min/mg Km for MKP3 is set to 267 nM, twice as high as MKP1 version, to lessen substrate saturation.

MKP-3 acting as a Product of an Enzyme in Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
MKP3_synthesis / kenz	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	MKP3 Pathway No. : 345	0.199998	0.01	4	explicit E-S complex	Substrate MKP3_AA Product MKP-3

MKP-3 acting as a Substrate in a reaction in Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
MKP3-turnover	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	MKP3 Pathway No. : 345	0.0005 (s^-1)	0 (s^-1)	-	-	Substrate MKP-3 Product ubiquitination

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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