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Molecule Parameter List for temp-PIP2

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
temp-PIP2 participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000020

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Ajay_Bhalla_
    2004_Feedback_
    Tuning
    		• 78Network
    Shared_Object_Ajay_Bhalla_2004_Feedback_Tuning,  PKC,  PLA2,  
    PLCbeta,  Gq,  MAPK,  
    Ras,  EGFR,  Sos,  
    PLC_g,  CaMKII,  CaM,  
    PP1,  PP2B,  PKA,  
    AC 
    This model is taken from Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80. This is the feedback model from Figure 8a.

    temp-PIP2 acting as a Molecule in      Ajay_Bhalla_2004_Feedback_Tuning Network
    NameAccession NamePathway NameInitial Conc.
    (uM)    		Volume
    (fL)    		Buffered
    temp-PIP2
  • Ajay_Bhalla_
    2004_Feedback_
    Tuning
    Accession No. : 78
  • Shared_Object_
    Ajay_Bhalla_
    2004_Feedback_
    Tuning
    Pathway No. : 347
    		• 2.51.5Yes
    This isn't explicitly present in the M&L model, but is obviously needed. I assume its conc is fixed at 1uM for now, which is a bit high. PLA2 is stim 7x by PIP2 @ 0.5 uM (Leslie and Channon BBA 1045:261(1990) Leslie and Channon say PIP2 is present at 0.1 - 0.2mol% range in membs, which comes to 50 nM. Ref is Majerus et al Cell 37 pp 701-703 1984 Lets use a lower level of 30 nM, same ref....

    temp-PIP2 acting as a Substrate in a reaction in      Ajay_Bhalla_2004_Feedback_Tuning Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1PIP2-PLA2-act
  • Ajay_Bhalla_
    2004_Feedback_
    Tuning
    Accession No. : 78
    		• PLA2
    Pathway No. : 349    		0.0012
    (uM^-1 s^-1)    		0.5
    (s^-1)    		Kd(bf) = 416.6771(uM)-Substrate
    PLA2-cytosolic
    temp-PIP2

    Product
    PIP2-PLA2*
    2
  • PIP2-Ca-PLA2-act
  • Ajay_Bhalla_
    2004_Feedback_
    Tuning
    Accession No. : 78
    		• PLA2
    Pathway No. : 349    		0.012
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 8.3335(uM)-Substrate
    PLA2-Ca*
    temp-PIP2

    Product
    PIP2-Ca-PLA2*


    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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