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Molecule Parameter List for DAG-Ca-PLA2* | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | Ajay_Bhalla_
2007_Bistable | 79 | Network |
Shared_Object_Ajay_Bhalla_2007_Bistable, PKC, PLA2,
MAPK, Ras, CaM | This is a model of ERKII signaling which is bistable due to feedback. The feedback occurs through ERKII phosphorylation of phospholipase A2 (PLA2), leading to increased production of arachidonic acid (AA), which activates protein kinase C (PKC) which activates c-Raf which is upstream of ERKII.
The model is a highly simplified variant of more detailed bistable models of MAPK signaling (Bhalla US, Iyengar R. Science. 1999 Jan 15;283(5400):381-7, Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80) |
DAG-Ca-PLA2* acting as a Molecule in Ajay_Bhalla_2007_Bistable Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | DAG-Ca-PLA2* | Ajay_Bhalla_
2007_Bistable
Accession No. : 79 | PLA2
Pathway No. : 365 | 0 | 125.7 | No |
DAG-Ca-PLA2* acting as an Enzyme in Ajay_Bhalla_2007_Bistable Network
Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | DAG-Ca-PLA2* /
kenz
| Ajay_Bhalla_
2007_Bistable
Accession No. : 79 | PLA2
Pathway No. : 365 | 19.9996 | 60 | 4 | explicit E-S complex | Substrate
APC
Product
AA
| | 10 X raise oct 22 12 X raise oct 24 + conversion to k2 =4 * k3 |
DAG-Ca-PLA2* acting as a Product in a reaction in Ajay_Bhalla_2007_Bistable Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | DAG-Ca-PLA2-act | Ajay_Bhalla_
2007_Bistable
Accession No. : 79 | PLA2
Pathway No. : 365 | 0.003
(uM^-1 s^-1) | 4
(s^-1) | Kd(bf) = 1333.3422(uM) | - | Substrate
DAG
PLA2-Ca*
Product
DAG-Ca-PLA2*
| | 27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2. |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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