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Molecule Parameter List for DAG

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

DAG participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	0	3	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
3d_fold_model	8	Network	Shared_Object_3d_fold_model,  PKC,  MAPK,   PLA2,  Ras
This model is an annotated version of the synaptic signaling network. The primary reference is Bhalla US and Iyengar R. Science (1999) 283(5400):381-7 but several of the model pathways have been updated Bhalla US, Ram PT, Iyengar R. Science. 2002 Aug 9;297(5583):1018-23.

DAG acting as a Molecule in  3d_fold_model Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
DAG	3d_fold_model Accession No. : 8	Shared_Object_ 3d_fold_model Pathway No. : 54	11.661	1000	Yes
Baseline is 11.661 from running combined model to steady state. See ALL/NOTES for 23 Apr 1998.

DAG acting as a Substrate in a reaction in  3d_fold_model Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	PKC-act-by-DAG	3d_fold_model Accession No. : 8	PKC Pathway No. : 55	0.008 (uM^-1 s^-1)	8.6348 (s^-1)	Kd(bf) = 1079.377(uM)	-	Substrate DAG PKC-Ca Product PKC-Ca-DAG
	Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
2	PKC-n-DAG	3d_fold_model Accession No. : 8	PKC Pathway No. : 55	0.0006 (uM^-1 s^-1)	0.1 (s^-1)	Kd(bf) = 166.6667(uM)	-	Substrate DAG PKC-cytosolic Product PKC-DAG
	kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
3	DAG-Ca-PLA2-act	3d_fold_model Accession No. : 8	PLA2 Pathway No. : 57	0.003 (uM^-1 s^-1)	4 (s^-1)	Kd(bf) = 1333.3333(uM)	-	Substrate DAG PLA2-Ca* Product DAG-Ca-PLA2*
	27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.