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Molecule Parameter List for GTP-Ras

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*GTP-Ras* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	40	0	0	40	120	159	39

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Ajay_Bhalla_ 2007_ReacDiff2	83	Network	Shared_Object_Ajay_Bhalla_2007_ReacDiff, PKC, MAPK, Ras, CaM, PKM, chain, kinetics, PKC, MAPK, Ras, CaM, PKM, kinetics[1], PKC, MAPK, Ras, kinetics[3], CaM, PKM, kinetics[2], PKC, MAPK, Ras, CaM, PKM, PKC, MAPK, Ras, CaM, PKM, kinetics[4], PKC, MAPK, Ras, CaM, PKM, kinetics[5], PKC, MAPK, Ras, CaM, PKM, kinetics[6], PKC, MAPK, Ras, CaM, PKM, kinetics[7], PKC, MAPK, Ras, CaM, PKM, kinetics[8], PKC, MAPK, Ras, CaM, PKM, kinetics[9], PKC, MAPK, Ras, CaM, PKM, kinetics[10], PKC, MAPK, Ras, CaM, PKM, kinetics[11], PKC, MAPK, Ras, CaM, PKM, kinetics[12], PKC, MAPK, Ras, CaM, PKM, kinetics[13], PKC, MAPK, Ras, CaM, PKM, kinetics[14], PKC, MAPK, Ras, CaM, PKM, kinetics[15], PKC, MAPK, Ras, CaM, PKM, kinetics[16], PKC, MAPK, Ras, CaM, PKM, kinetics[17], PKC, MAPK, Ras, CaM, PKM, kinetics[18], PKC, MAPK, Ras, CaM, PKM, kinetics[19], PKC, MAPK, Ras, CaM, PKM, kinetics[20], PKC, MAPK, Ras, CaM, PKM, kinetics[21], PKC, MAPK, Ras, CaM, PKM, kinetics[22], PKC, MAPK, Ras, CaM, PKM, kinetics[23], PKC, MAPK, Ras, CaM, PKM, kinetics[24], PKC, MAPK, Ras, CaM, PKM, kinetics[25], PKC, MAPK, Ras, CaM, PKM, kinetics[26], PKC, MAPK, Ras, CaM, PKM, kinetics[27], PKC, MAPK, Ras, CaM, PKM, kinetics[28], PKC, MAPK, Ras, CaM, PKM, kinetics[29], PKC, MAPK, Ras, CaM, PKM, kinetics[30], PKC, MAPK, Ras, CaM, PKM, kinetics[31], PKC, MAPK, Ras, CaM, PKM, kinetics[32], PKC, MAPK, Ras, CaM, PKM, kinetics[33], PKC, MAPK, Ras, CaM, PKM, kinetics[34], PKC, MAPK, Ras, CaM, PKM, kinetics[35], PKC, MAPK, Ras, CaM, PKM, kinetics[36], PKC, MAPK, Ras, CaM, PKM, kinetics[37], PKC, MAPK, Ras, CaM, PKM, kinetics[38], PKC, MAPK, Ras, CaM, PKM
This is a 40-compartment reaction-diffusion-transport version of the Ajay_Bhalla_2007_PKM model. The original single-compartment model is repeated 40 times. In addition, a subset (27 out of 42) molecules can diffuse between compartments. Diffusion is implemented as a reaction between corresponding molecules in neighboring compartments. For D = 1e-12 m^2/sec (i.e., 1 micron^2/sec ) the kf and kb of this reaction for these 10 micron compartments are both 0.01/sec In addition, we have a forward (dendrite to soma) transport term of 1 microns/sec. This converts to a rate of 0.1/sec, but applies only to the kf. So the total kf of the diffusion 'reaction' is 0.11 for D = 1 micron^2/sec, and kb is 0.01. If D=0.1 micron^2/sec then kf = 0.101 and kb = 0.001. In addition this model has all molecules buffered in the first and last compartments. This boundary conditions says that the molecules are not drained out of the first compartment, nor do they all pile up in the last one. The stimulus file pkm_mapk22_transp_endbuf_D1e-13_Fig4CD which was used for the model to replicate Figure 4C and 4D from the paper.

GTP-Ras acting as a Molecule in Ajay_Bhalla_2007_ReacDiff2 Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 687	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 693	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 700	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 705	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 711	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 717	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 723	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 729	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 735	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 741	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 747	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 753	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 759	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 765	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 771	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 777	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 783	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 789	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 795	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 801	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 807	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 813	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 819	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 825	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 831	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 837	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 843	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 849	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 855	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 861	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 867	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 873	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 879	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 885	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 891	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 897	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 903	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 909	0.0035	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 680	0.0035	1.5	Yes
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 915	0.0035	1.5	Yes
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437

GTP-Ras acting as a Substrate for an Enzyme in Ajay_Bhalla_2007_ReacDiff2 Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 680	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
2	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 687	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
3	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 693	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
4	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 700	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
5	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 705	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
6	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 711	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
7	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 717	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
8	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 723	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
9	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 729	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
10	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 735	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
11	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 741	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
12	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 747	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
13	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 753	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
14	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 759	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
15	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 765	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
16	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 771	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
17	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 777	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
18	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 783	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
19	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 789	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
20	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 795	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
21	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 801	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
22	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 807	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
23	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 813	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
24	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 819	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
25	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 825	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
26	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 831	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
27	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 837	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
28	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 843	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
29	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 849	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
30	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 855	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
31	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 861	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
32	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 867	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
33	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 873	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
34	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 879	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
35	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 885	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
36	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 891	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
37	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 897	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
38	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 903	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
39	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 909	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
40	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 915	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.

GTP-Ras acting as a Product of an Enzyme in Ajay_Bhalla_2007_ReacDiff2 Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 680	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

2	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 680	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
3	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 680	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
4	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 687	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

5	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 687	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
6	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 687	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
7	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 693	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

8	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 693	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
9	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 693	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
10	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 700	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

11	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 700	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
12	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 700	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
13	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 705	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

14	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 705	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
15	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 705	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
16	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 711	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

17	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 711	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
18	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 711	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
19	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 717	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

20	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 717	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
21	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 717	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
22	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 723	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

23	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 723	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
24	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 723	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
25	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 729	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

26	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 729	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
27	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 729	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
28	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 735	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

29	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 735	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
30	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 735	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
31	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 741	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

32	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 741	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
33	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 741	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
34	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 747	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

35	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 747	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
36	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 747	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
37	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 753	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

38	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 753	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
39	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 753	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
40	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 759	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

41	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 759	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
42	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 759	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
43	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 765	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

44	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 765	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
45	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 765	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
46	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 771	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

47	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 771	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
48	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 771	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
49	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 777	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

50	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 777	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
51	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 777	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
52	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 783	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

53	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 783	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
54	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 783	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
55	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 789	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

56	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 789	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
57	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 789	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
58	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 795	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

59	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 795	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
60	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 795	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
61	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 801	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

62	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 801	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
63	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 801	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
64	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 807	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

65	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 807	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
66	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 807	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
67	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 813	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

68	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 813	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
69	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 813	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
70	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 819	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

71	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 819	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
72	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 819	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
73	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 825	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

74	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 825	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
75	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 825	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
76	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 831	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

77	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 831	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
78	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 831	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
79	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 837	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

80	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 837	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
81	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 837	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
82	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 843	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

83	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 843	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
84	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 843	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
85	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 849	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

86	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 849	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
87	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 849	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
88	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 855	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

89	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 855	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
90	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 855	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
91	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 861	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

92	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 861	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
93	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 861	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
94	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 867	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

95	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 867	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
96	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 867	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
97	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 873	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

98	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 873	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
99	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 873	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
100	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 879	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

101	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 879	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
102	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 879	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
103	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 885	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

104	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 885	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
105	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 885	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
106	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 891	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

107	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 891	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
108	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 891	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
109	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 897	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

110	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 897	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
111	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 897	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
112	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 903	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

113	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 903	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
114	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 903	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
115	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 909	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

116	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 909	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
117	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 909	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
118	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 915	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

119	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 915	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
120	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 915	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.

GTP-Ras acting as a Substrate in a reaction in Ajay_Bhalla_2007_ReacDiff2 Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
2	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
3	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 680	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
4	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
5	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics Pathway No. : 684	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
6	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
7	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 687	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
8	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics Pathway No. : 684	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
9	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[1] Pathway No. : 690	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
10	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
11	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 693	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
12	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[1] Pathway No. : 690	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
13	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[2] Pathway No. : 697	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
14	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
15	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 700	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
16	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[2] Pathway No. : 697	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
17	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[3] Pathway No. : 694	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
18	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
19	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 705	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
20	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[3] Pathway No. : 694	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
21	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[4] Pathway No. : 708	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
22	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
23	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 711	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
24	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[4] Pathway No. : 708	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
25	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[5] Pathway No. : 714	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
26	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
27	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 717	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
28	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[5] Pathway No. : 714	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
29	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[6] Pathway No. : 720	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
30	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
31	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 723	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
32	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[6] Pathway No. : 720	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
33	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[7] Pathway No. : 726	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
34	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
35	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 729	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
36	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[7] Pathway No. : 726	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
37	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[8] Pathway No. : 732	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
38	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
39	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 735	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
40	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[8] Pathway No. : 732	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
41	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[9] Pathway No. : 738	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
42	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
43	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 741	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
44	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[9] Pathway No. : 738	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
45	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[10] Pathway No. : 744	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
46	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
47	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 747	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
48	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[10] Pathway No. : 744	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
49	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[11] Pathway No. : 750	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
50	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
51	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 753	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
52	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[11] Pathway No. : 750	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
53	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[12] Pathway No. : 756	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
54	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
55	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 759	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
56	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[12] Pathway No. : 756	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
57	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[13] Pathway No. : 762	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
58	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
59	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 765	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
60	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[13] Pathway No. : 762	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
61	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[14] Pathway No. : 768	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
62	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
63	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 771	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
64	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[14] Pathway No. : 768	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
65	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[15] Pathway No. : 774	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
66	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
67	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 777	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
68	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[15] Pathway No. : 774	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
69	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[16] Pathway No. : 780	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
70	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
71	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 783	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
72	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[16] Pathway No. : 780	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
73	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[17] Pathway No. : 786	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
74	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
75	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 789	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
76	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[17] Pathway No. : 786	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
77	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[18] Pathway No. : 792	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
78	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
79	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 795	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
80	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[18] Pathway No. : 792	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
81	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[19] Pathway No. : 798	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
82	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
83	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 801	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
84	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[19] Pathway No. : 798	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
85	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[20] Pathway No. : 804	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
86	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
87	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 807	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
88	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[20] Pathway No. : 804	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
89	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[21] Pathway No. : 810	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
90	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
91	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 813	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
92	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[21] Pathway No. : 810	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
93	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[22] Pathway No. : 816	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
94	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
95	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 819	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
96	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[22] Pathway No. : 816	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
97	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[23] Pathway No. : 822	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
98	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
99	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 825	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
100	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[23] Pathway No. : 822	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
101	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[24] Pathway No. : 828	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
102	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
103	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 831	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
104	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[24] Pathway No. : 828	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
105	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[25] Pathway No. : 834	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
106	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
107	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 837	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
108	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[25] Pathway No. : 834	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
109	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[26] Pathway No. : 840	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
110	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
111	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 843	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
112	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[26] Pathway No. : 840	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
113	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[27] Pathway No. : 846	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
114	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
115	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 849	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
116	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[27] Pathway No. : 846	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
117	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[28] Pathway No. : 852	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
118	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
119	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 855	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
120	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[28] Pathway No. : 852	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
121	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[29] Pathway No. : 858	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
122	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
123	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 861	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
124	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[29] Pathway No. : 858	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
125	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[30] Pathway No. : 864	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
126	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
127	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 867	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
128	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[30] Pathway No. : 864	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
129	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[31] Pathway No. : 870	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
130	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
131	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 873	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
132	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[31] Pathway No. : 870	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
133	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[32] Pathway No. : 876	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
134	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
135	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 879	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
136	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[32] Pathway No. : 876	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
137	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[33] Pathway No. : 882	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
138	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
139	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 885	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
140	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[33] Pathway No. : 882	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
141	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[34] Pathway No. : 888	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
142	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
143	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 891	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
144	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[34] Pathway No. : 888	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
145	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[35] Pathway No. : 894	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
146	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
147	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 897	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
148	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[35] Pathway No. : 894	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
149	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[36] Pathway No. : 900	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
150	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
151	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 903	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
152	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[36] Pathway No. : 900	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
153	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[37] Pathway No. : 906	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
154	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
155	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 909	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
156	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[37] Pathway No. : 906	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
157	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[38] Pathway No. : 912	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
158	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Ras Pathway No. : 915	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
159	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[38] Pathway No. : 912	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.

GTP-Ras acting as a Product in a reaction in Ajay_Bhalla_2007_ReacDiff2 Network

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
2	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
3	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
4	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
5	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
6	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
7	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
8	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
9	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
10	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
11	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
12	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
13	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
14	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
15	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
16	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
17	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
18	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
19	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
20	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
21	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
22	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
23	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
24	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
25	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
26	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
27	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
28	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
29	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
30	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
31	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
32	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
33	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
34	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
35	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
36	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
37	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
38	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras
39	diff	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.101 (s^-1)	0.001 (s^-1)	Keq = 0.0099(uM)	9.804sec	Substrate GTP-Ras Product GTP-Ras

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