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Molecule Parameter List for CaM

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
CaM participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences4000007939

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Ajay_Bhalla_
    2007_ReacDiff2
    		• 83NetworkShared_Object_Ajay_Bhalla_2007_ReacDiff,  PKC,  MAPK,  
    Ras,  CaM,  PKM,  chain,  kinetics,  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[1],  
    PKC,  MAPK,  Ras,  kinetics[3],  CaM,  PKM,  kinetics[2],  PKC,  MAPK,  Ras,  
    CaM,  PKM,  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[4],  PKC,  MAPK,  Ras,  CaM,  
    PKM,  kinetics[5],  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[6],  PKC,  MAPK,  
    Ras,  CaM,  PKM,  kinetics[7],  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[8],  
    PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[9],  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[10],  
    PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[11],  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[12],  
    PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[13],  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[14],  
    PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[15],  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[16],  
    PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[17],  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[18],  
    PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[19],  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[20],  
    PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[21],  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[22],  
    PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[23],  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[24],  
    PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[25],  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[26],  
    PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[27],  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[28],  
    PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[29],  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[30],  
    PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[31],  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[32],  
    PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[33],  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[34],  
    PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[35],  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[36],  
    PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[37],  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[38],  
    PKC,  MAPK,  Ras,  CaM,  PKM 
    This is a 40-compartment reaction-diffusion-transport version of the Ajay_Bhalla_2007_PKM model. The original single-compartment model is repeated 40 times. In addition, a subset (27 out of 42) molecules can diffuse between compartments. Diffusion is implemented as a reaction between corresponding molecules in neighboring compartments. For D = 1e-12 m^2/sec (i.e., 1 micron^2/sec ) the kf and kb of this reaction for these 10 micron compartments are both 0.01/sec In addition, we have a forward (dendrite to soma) transport term of 1 microns/sec. This converts to a rate of 0.1/sec, but applies only to the kf. So the total kf of the diffusion 'reaction' is 0.11 for D = 1 micron^2/sec, and kb is 0.01. If D=0.1 micron^2/sec then kf = 0.101 and kb = 0.001. In addition this model has all molecules buffered in the first and last compartments. This boundary conditions says that the molecules are not drained out of the first compartment, nor do they all pile up in the last one.
    The stimulus file pkm_mapk22_transp_endbuf_D1e-13_Fig4CD which was used for the model to replicate Figure 4C and 4D from the paper.

    CaM acting as a Molecule in      Ajay_Bhalla_2007_ReacDiff2 Network
    NameAccession NamePathway NameInitial Conc.
    (uM)    		Volume
    (fL)    		Buffered
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 688    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 695    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 701    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 706    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 712    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 718    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 724    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 730    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 736    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 742    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 748    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 754    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 760    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 766    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 772    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 778    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 784    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 790    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 796    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 802    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 808    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 814    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 820    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 826    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 832    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 838    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 844    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 850    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 856    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 862    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 868    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 874    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 880    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 886    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 892    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 898    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 904    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 910    		18.40281.5No
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 681    		18.40281.5Yes
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
    CaM
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 916    		18.40281.5Yes
    There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.

    CaM acting as a Substrate in a reaction in      Ajay_Bhalla_2007_ReacDiff2 Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    2CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 681    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    3diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    4CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 688    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    5diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    6CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 695    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    7diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    8CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 701    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    9diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    10CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 706    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    11diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    12CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 712    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    13diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    14CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 718    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    15diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    16CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 724    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    17diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    18CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 730    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    19diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    20CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 736    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    21diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    22CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 742    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    23diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    24CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 748    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    25diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    26CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 754    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    27diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    28CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 760    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    29diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    30CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 766    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    31diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    32CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 772    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    33diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    34CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 778    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    35diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    36CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 784    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    37diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    38CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 790    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    39diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    40CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 796    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    41diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    42CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 802    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    43diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    44CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 808    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    45diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    46CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 814    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    47diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    48CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 820    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    49diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    50CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 826    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    51diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    52CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 832    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    53diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    54CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 838    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    55diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    56CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 844    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    57diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    58CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 850    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    59diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    60CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 856    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    61diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    62CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 862    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    63diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    64CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 868    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    65diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    66CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 874    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    67diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    68CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 880    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    69diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    70CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 886    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    71diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    72CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 892    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    73diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    74CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 898    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    75diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    76CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 904    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    77diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    78CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 910    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    79CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
    		• CaM
    Pathway No. : 916    		8.4851
    (uM^-1 s^-1)    		8.4853
    (s^-1)    		Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853

    CaM acting as a Product in a reaction in      Ajay_Bhalla_2007_ReacDiff2 Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    2diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    3diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    4diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    5diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    6diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    7diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    8diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    9diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    10diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    11diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    12diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    13diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    14diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    15diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    16diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    17diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    18diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    19diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    20diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    21diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    22diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    23diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    24diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    25diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    26diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    27diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    28diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    29diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    30diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    31diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    32diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    33diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    34diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    35diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    36diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    37diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    38diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM
    39diff
  • Ajay_Bhalla_
    2007_ReacDiff2
    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff
    Pathway No. : 677
    		• 0.101
    (s^-1)    		0.001
    (s^-1)    		Keq = 0.0099(uM)9.804secSubstrate
    CaM

    Product
    CaM


    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
    This Copyright is applied to ensure that the contents of this database remain freely available. Please see FAQ for details.