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Molecule Parameter List for CaM-Ca

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
CaM-Ca participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences4000007979

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Ajay_Bhalla_
    2007_ReacDiff2
  • 83NetworkShared_Object_Ajay_Bhalla_2007_ReacDiff PKC MAPK 
    Ras CaM PKM chain kinetics PKC MAPK Ras CaM PKM kinetics[1] 
    PKC MAPK Ras kinetics[3] CaM PKM kinetics[2] PKC MAPK Ras 
    CaM PKM PKC MAPK Ras CaM PKM kinetics[4] PKC MAPK Ras CaM 
    PKM kinetics[5] PKC MAPK Ras CaM PKM kinetics[6] PKC MAPK 
    Ras CaM PKM kinetics[7] PKC MAPK Ras CaM PKM kinetics[8] 
    PKC MAPK Ras CaM PKM kinetics[9] PKC MAPK Ras CaM PKM kinetics[10] 
    PKC MAPK Ras CaM PKM kinetics[11] PKC MAPK Ras CaM PKM kinetics[12] 
    PKC MAPK Ras CaM PKM kinetics[13] PKC MAPK Ras CaM PKM kinetics[14] 
    PKC MAPK Ras CaM PKM kinetics[15] PKC MAPK Ras CaM PKM kinetics[16] 
    PKC MAPK Ras CaM PKM kinetics[17] PKC MAPK Ras CaM PKM kinetics[18] 
    PKC MAPK Ras CaM PKM kinetics[19] PKC MAPK Ras CaM PKM kinetics[20] 
    PKC MAPK Ras CaM PKM kinetics[21] PKC MAPK Ras CaM PKM kinetics[22] 
    PKC MAPK Ras CaM PKM kinetics[23] PKC MAPK Ras CaM PKM kinetics[24] 
    PKC MAPK Ras CaM PKM kinetics[25] PKC MAPK Ras CaM PKM kinetics[26] 
    PKC MAPK Ras CaM PKM kinetics[27] PKC MAPK Ras CaM PKM kinetics[28] 
    PKC MAPK Ras CaM PKM kinetics[29] PKC MAPK Ras CaM PKM kinetics[30] 
    PKC MAPK Ras CaM PKM kinetics[31] PKC MAPK Ras CaM PKM kinetics[32] 
    PKC MAPK Ras CaM PKM kinetics[33] PKC MAPK Ras CaM PKM kinetics[34] 
    PKC MAPK Ras CaM PKM kinetics[35] PKC MAPK Ras CaM PKM kinetics[36] 
    PKC MAPK Ras CaM PKM kinetics[37] PKC MAPK Ras CaM PKM kinetics[38] 
    PKC MAPK Ras CaM PKM 
    This is a 40-compartment reaction-diffusion-transport version of the Ajay_Bhalla_2007_PKM model. The original single-compartment model is repeated 40 times. In addition, a subset (27 out of 42) molecules can diffuse between compartments. Diffusion is implemented as a reaction between corresponding molecules in neighboring compartments. For D = 1e-12 m^2/sec (i.e., 1 micron^2/sec ) the kf and kb of this reaction for these 10 micron compartments are both 0.01/sec In addition, we have a forward (dendrite to soma) transport term of 1 microns/sec. This converts to a rate of 0.1/sec, but applies only to the kf. So the total kf of the diffusion 'reaction' is 0.11 for D = 1 micron^2/sec, and kb is 0.01. If D=0.1 micron^2/sec then kf = 0.101 and kb = 0.001. In addition this model has all molecules buffered in the first and last compartments. This boundary conditions says that the molecules are not drained out of the first compartment, nor do they all pile up in the last one.
    The stimulus file pkm_mapk22_transp_endbuf_D1e-13_Fig4CD which was used for the model to replicate Figure 4C and 4D from the paper.

    CaM-Ca acting as a Molecule in  
    Ajay_Bhalla_2007_ReacDiff2 Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 688
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 695
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 701
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 706
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 712
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 718
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 724
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 730
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 736
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 742
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 748
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 754
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 760
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 766
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 772
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 778
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 784
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 790
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 796
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 802
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 808
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 814
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 820
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 826
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 832
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 838
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 844
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 850
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 856
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 862
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 868
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 874
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 880
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 886
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 892
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 898
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 904
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 910
    1.4721.5No
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 681
    1.4721.5Yes
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.
    CaM-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 916
    1.4721.5Yes
    This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not.

    CaM-Ca acting as a Substrate in a reaction in  
    Ajay_Bhalla_2007_ReacDiff2 Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 681
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    2diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    3CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 688
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    4diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    5CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 695
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    6diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    7CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 701
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    8diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    9CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 706
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    10diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    11CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 712
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    12diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    13CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 718
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    14diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    15CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 724
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    16diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    17CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 730
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    18diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    19CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 736
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    20diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    21CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 742
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    22diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    23CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 748
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    24diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    25CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 754
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    26diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    27CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 760
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    28diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    29CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 766
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    30diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    31CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 772
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    32diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    33CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 778
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    34diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    35CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 784
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    36diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    37CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 790
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    38diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    39CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 796
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    40diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    41CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 802
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    42diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    43CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 808
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    44diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    45CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 814
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    46diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    47CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 820
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    48diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    49CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 826
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    50diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    51CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 832
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    52diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    53CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 838
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    54diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    55CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 844
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    56diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    57CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 850
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    58diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    59CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 856
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    60diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    61CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 862
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    62diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    63CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 868
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    64diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    65CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 874
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    66diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    67CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 880
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    68diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    69CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 886
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    70diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    71CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 892
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    72diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    73CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 898
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    74diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    75CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 904
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    76diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    77CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 910
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    78diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    79CaM-Ca-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 916
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM-Ca

    Product
    CaM-Ca2
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853

    CaM-Ca acting as a Product in a reaction in  
    Ajay_Bhalla_2007_ReacDiff2 Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 681
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    2diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    3CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 688
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    4diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    5CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 695
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    6diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    7CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 701
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    8diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    9CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 706
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    10diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    11CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 712
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    12diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    13CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 718
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    14diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    15CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 724
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    16diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    17CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 730
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    18diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    19CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 736
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    20diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    21CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 742
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    22diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    23CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 748
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    24diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    25CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 754
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    26diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    27CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 760
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    28diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    29CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 766
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    30diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    31CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 772
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    32diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    33CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 778
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    34diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    35CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 784
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    36diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    37CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 790
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    38diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    39CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 796
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    40diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    41CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 802
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    42diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    43CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 808
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    44diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    45CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 814
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    46diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    47CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 820
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    48diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    49CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 826
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    50diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    51CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 832
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    52diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    53CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 838
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    54diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    55CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 844
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    56diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    57CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 850
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    58diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    59CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 856
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    60diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    61CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 862
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    62diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    63CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 868
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    64diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    65CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 874
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    66diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    67CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 880
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    68diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    69CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 886
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    70diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    71CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 892
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    72diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    73CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 898
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    74diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    75CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 904
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    76diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    77CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 910
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
    78diff
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff

    Pathway No. : 677
  • 0.101
    (s^-1)
    0.001
    (s^-1)
    Keq = 0.0099(uM)9.804secSubstrate
    CaM-Ca

    Product
    CaM-Ca
    79CaM-bind-Ca
  • Ajay_Bhalla_
    2007_ReacDiff2

    Accession No. : 83
  • CaM
    Pathway No. : 916
    8.4851
    (uM^-1 s^-1)
    8.4853
    (s^-1)
    Kd(bf) = 1(uM)-Substrate
    Ca
    CaM

    Product
    CaM-Ca
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853



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