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Molecule Parameter List for E2FAP | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | Mammalian_cell_
cycle | 85 | Network |
Growth, CELLDIV, Rb_grp,
IE_GRP, CycB_Grp, Cdc20_Grp,
Cdh1_grp, E2F, CycA_Grp,
CycE_grp, Early_Response_Genes, Delayed_Response_Genes,
CycD_Grp | | This is a fairly complete mass-action reimplementation of the Novak and Tyson mammalian cell cycle model. It is inexact on two counts. First, it replaces many rather abstracted equations with mass action and Michaelis-Menten forms of enzymes. Second, it does not handle the halving of cellular volume at the division point. Within these limitations, the model does most of what the original paper shows including oscillation of the relevant molecules. |
E2FAP acting as a Molecule in Mammalian_cell_cycle Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | E2FAP | Mammalian_cell_
cycle
Accession No. : 85 | E2F
Pathway No. : 1076 | 0 | 200 | No |
E2FAP acting as a Product of an Enzyme in Mammalian_cell_cycle Network
| | Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | | 1 | CycD /
k20_lambdaD[1] | Mammalian_cell_
cycle
Accession No. : 85 | CELLDIV
Pathway No. : 1070 | 100 | 3300 | 4 | explicit E-S complex | Substrate
E2FAP.Rb
Product
E2FAP
Rb_P
| | | With a low Km, rate ~ kcat. Here we have rate = k20 * lambda_d = 10 * 3.3 = 33. 7 Apr 2005. Actually should have the substrate term in here. Use the form Km >> substrate, so rate = kcat * sub * enz / Km so kcat = Km * k20 * lambda_d = 10 * 10 * 3.3 = 330 The idea here is that these reactions phosphorylate the Rb protein attached to E2FAP, so that Rb_P is released and E2FAP is left. | | 2 | CycE /
k20_lambdaE[1] | Mammalian_cell_
cycle
Accession No. : 85 | CELLDIV
Pathway No. : 1070 | 100.002 | 5000 | 4 | explicit E-S complex | Substrate
E2FAP.Rb
Product
E2FAP
Rb_P
| | | For Km ~ 0, rate ~ kcat. rate = k20 * lambdaE = 10 * 5 7 Apr 2005. Actually need to put in substrate term too. Let Km = 10 >> sub. Then, rate ~ kcat * sub * prd /Km so kcat = Km * k20 * lambdaE = 10 * 10 * 5 = 500 | | 3 | CycA /
A_phosph_E2F | Mammalian_cell_
cycle
Accession No. : 85 | CELLDIV
Pathway No. : 1070 | 9.99992 | 10 | 4 | explicit E-S complex | Substrate
E2FA
Product
E2FAP
| | | Rate equn has form [CycA].[E2F].k23 k23 = 1 MM equn has form [CycA].[E2F].kcat/(Km + E2F) So, we set kcat = Km * k23 where Km >> E2F 25 Mar. Better: Use explicit enz form. rate = k3.k1/k2 if k3 << k2. Let k3 = 1, k2 = 10, so we get k1 = k23 * 10 = 10. 6 Apr. Problem with explicit form is that the enz-substrate complex may affect the levels of the CycA, B etc. Back to MM. | | 4 | CycA /
k20_lambdaA[1] | Mammalian_cell_
cycle
Accession No. : 85 | CELLDIV
Pathway No. : 1070 | 100 | 3000 | 4 | explicit E-S complex | Substrate
E2FAP.Rb
Product
E2FAP
Rb_P
| | | Km ~ 0, so rate ~ kcat. Here rate = k20 * lambdaA = 10 * 3 7 Apr 2005: Fix it: rate should have substrate term in it. Set Km = 10 >> substrate. Then, kcat = Km * k20 * lambdaA = 10 * 10 * 3 = 300 | | 5 | CycB /
B_phosph_E2FA | Mammalian_cell_
cycle
Accession No. : 85 | CELLDIV
Pathway No. : 1070 | 9.99992 | 10 | 4 | explicit E-S complex | Substrate
E2FA
Product
E2FAP
| | | See A_phosph_E2F. Same rate of k23 = 1 applies. | | 6 | CycB /
k20_lambdaB[1] | Mammalian_cell_
cycle
Accession No. : 85 | CELLDIV
Pathway No. : 1070 | 100.002 | 5000 | 4 | explicit E-S complex | Substrate
E2FAP.Rb
Product
E2FAP
Rb_P
| | | With Km ~ 0, rate ~ kcat. Here rate = k20 * lambdaB = 10 * 5 7 Apr 2005. Changed to include substrate term. Use Km = 10 >> sub, so kcat = Km * k20 * lambdaB = 10 * 10 * 5 = 500 |
E2FAP acting as a Substrate in a reaction in Mammalian_cell_cycle Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| | Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | 1 | k22_23_prime | Mammalian_cell_
cycle
Accession No. : 85 | E2F
Pathway No. : 1076 | 1
(s^-1) | 0.005
(s^-1) | Keq = 0.005(uM) | 0.995sec | Substrate
E2FAP
Product
E2FA
| | | k22 is the forward rate of 1 k23_prime is the backward rate of 0.005 | | 2 | k26_P | Mammalian_cell_
cycle
Accession No. : 85 | E2F
Pathway No. : 1076 | 10
(uM^-1 s^-1) | 200
(s^-1) | Kd(bf) = 20.0001(uM) | - | Substrate
E2FAP
Rb
Product
E2FAP.Rb
| | | Same as k26, but acting on the phosph form. |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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