|
Name | Accession Type | Initial Conc. (uM) | Volume (fL) | Buffered | Sum Total Of |
1 | CaM | Network | 26.3333 | 0 | No | - |
| There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels. |
2 | neurogranin-CaM | Network | 0 | 0 | No | - |
3 | neurogranin* | Network | 0 | 0 | No | - |
| The phosph form does not bind CaM (Huang et al ABB 305:2 570-580 1993) |
4 | neurogranin | Network | 10 | 0 | No | - |
| Also known as RC3 and p17 and BICKS. Conc in brain >> 2 uM from Martzen and Slemmon J neurosci 64 92-100 1995 but others say less without any #s. Conc in dend spines is much higher than overall, so it could be anywhere from 2 uM to 50. We will estimate 10 uM as a starting point. Gerendasy et al JBC 269:35 22420-22426 1994 have a skeleton model (no numbers) indicating CaM-Ca(n) binding. |
5 | CaM-PSD | Network | 26.3333 | 0 | No | - |
| There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels. |
6 | neurogranin-CaM_ PSD | Network | 0 | 0 | No | - |
7 | neurogranin_PSD | Network | 10 | 0 | No | - |
| Also known as RC3 and p17 and BICKS. Conc in brain >> 2 uM from Martzen and Slemmon J neurosci 64 92-100 1995 but others say less without any #s. Conc in dend spines is much higher than overall, so it could be anywhere from 2 uM to 50. We will estimate 10 uM as a starting point. Gerendasy et al JBC 269:35 22420-22426 1994 have a skeleton model (no numbers) indicating CaM-Ca(n) binding. |
8 | neurogranin*_ PSD | Network | 0 | 0 | No | - |
| The phosph form does not bind CaM (Huang et al ABB 305:2 570-580 1993) |
9 | CaM-TR2-Ca2 | Network | 0 | 0 | No | - |
| This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. |
10 | CaM-Ca3 | Network | 0 | 0 | No | - |
11 | CaM-Ca4-PSD | Network | 0 | 0 | No | - |
12 | CaM-Ca3-PSD | Network | 0 | 0 | No | - |
13 | CaM-TR2-Ca2-PSD | Network | 0 | 0 | No | - |
| This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. |
14 | CaM-Ca4 | Network | 0 | 0 | No | - |