| | Molecule Name/
Site Name | Km (uM) | kcat (1/s) | Ratio
(k2/k3) | Enzyme Type | Substrate | Product |
| 1 |
Enzyme Activity:
ip3-3k
Enzyme Molecule:
IP3_3K | 1.40001 | 3.0475 | 4 | explicit E-S complex | IP3(145)
| IP4(1345)
|
| | from Johanson et al, JBC 263; 1988 Original Vmax scaled up by 50% to obtain value at 37C, as enzyme assay was done at 30C Km increased from 0.2 to 1.4 as per various other reports (Shears Review, BiochemJ 260; 1989) |
| 2 |
Enzyme Activity:
ip3-3k*
Enzyme Molecule:
IP3_3K* | 1.40001 | 3.0475 | 4 | explicit E-S complex | IP3(145)
| IP4(1345)
|
| | from Communi et al, EMBO J 16; 1997 In absence of CaM binding, activity same as that of non-phosphorylated enzyme |
| 3 |
Enzyme Activity:
ip3-3k*1
Enzyme Molecule:
IP3_3K*1 | 1.40004 | 0.76187 | 4.00003 | explicit E-S complex | IP3(145)
| IP4(1345)
|
| | from Sim et al, JBC 265; 1990 |
| 4 |
Enzyme Activity:
ip3-3k-CaM
Enzyme Molecule:
IP3_3K_CaM | 2.8001 | 10.5 | 4.0001 | explicit E-S complex | IP3(145)
| IP4(1345)
|
| | from Erneux et al, Biochem 214; 1993 2-2.5 fold more active than ip3-3k but Km is doubled, hence Vmax adjusted accordingly. |
| 5 |
Enzyme Activity:
ip3-3k-CaM*
Enzyme Molecule:
IP3_3K_CaM* | 2.80002 | 45 | 4 | explicit E-S complex | IP3(145)
| IP4(1345)
|
| | from Communi et al, EMBO J 16; 1997 Vmax adjusted to obtain 9-fold greater activity than ip3-3k |
