| | Name | Initial Conc. (uM) | Volume (fL) | Buffered |
| 1 | Inositol | 0 | 1.5 | Yes |
| 2 | PC | 0 | 2.5E-6 | Yes |
| | Phosphatidylcholine is the main (around 55%) metabolic product of DAG, follwed by PE (around 25%). Ref is Welsh and Cabot, JCB35:231-245(1987) |
| 3 | PLC | 0.8 | 1.5 | No |
| | Total PLC = 0.8 uM see Ryu et al JBC 262 (26) pp 12511 1987 |
| 4 | PLC-Ca | 0 | 1.5 | No |
| 5 | PLC-Ca-Gq | 0 | 1.5 | No |
| | This should really be labelled PLC-G*GTP-Ca. This is the activated form of the enzyme. Mahama and Linderman assume that the IP3 precursors are not rate-limiting, but I will include those for completeness as they may be needed later. |
| 6 | PLC-Gq | 0 | 1.5 | No |
