DOQCS database

	Name	Initial Conc. (uM)	Volume (fL)	Buffered
1	AA	0	125.7	No
2	Ca	0.095	125.7	Yes
3	Ca_input	0.08	125.7	Yes
4	DAG	12.2	125.7	Yes
5	IP3	0.73	125.7	Yes
	Peak IP3 is perhaps 15 uM, basal <= 0.2 uM.
6	MAPK*	0	125.7	No
7	MKP-1	0.024	125.7	No
	MKP-1 dephosphoryates and inactivates MAPK in vivo: Sun et al Cell 75 487-493 1993. Levels of MKP-1 are regulated, and rise in 1 hour. Kinetics from Charles et al PNAS 90:5292-5296 1993. They refer to Charles et al Oncogene 7 187-190 who show that half-life of MKP1/3CH134 is 40 min. 80% deph of MAPK in 20 min Sep 17 1997: CoInit now 0.4x to 0.0032. See parm searches from jun96 on.
8	PKC-active	0.3	125.7	No
9	PPhosphatase2A	1	125.7	No
	Refs: Pato et al Biochem J 293:35-41(93); Takai&Mieskes Biochem J 275:233-239 k1=1.46e-4, k2=1000,k3=250. these use kcat values for calponin. Also, units of kcat may be in min! revert to Vmax base: k3=6, k2=25,k1=3.3e-6 or 6,6,1e-6 CoInit assumed 0.1 uM. See NOTES for MAPK_Ras50.g. CoInit now 0.08 Sep 17 1997: Raise CoInt 1.4x to 0.224, see parm searches from jun 96 on.
10	temp-PIP2	2.5	125.7	Yes
	This isn't explicitly present in the M&L model, but is obviously needed. I assume its conc is fixed at 1uM for now, which is a bit high. PLA2 is stim 7x by PIP2 @ 0.5 uM (Leslie and Channon BBA 1045:261(1990) Leslie and Channon say PIP2 is present at 0.1 - 0.2mol% range in membs, which comes to 50 nM. Ref is Majerus et al Cell 37 pp 701-703 1984 Lets use a lower level of 30 nM, same ref....

Summed Molecule List

	Target	Inputs
1	PKC-active	PKC-DAG-AA* PKC-Ca-AA* PKC-Ca-memb* PKC-DAG-memb* PKC-basal* PKC-AA*

Pathway Details Molecule List Enzyme List Reaction List

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