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Reaction List for pathway CaMKII (Pathway Number 245) | | Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reactions is not considered. |
| | Name | Kf | Kb | Kd | tau | Substrate | Product | | 1 |
back_1 | 0.3
(s^-1) | 0
(#^-1 s^-1) | Not applicable** | - | CaMKII-PSD
| CaMKII
NMDAR
| | | Rates set by the translocation experiments of Shen and Meyer, Science 1999. | | 2 |
back_2 | 0.3
(s^-1) | 0
(#^-1 s^-1) | Not applicable** | - | CaMKII-thr305-PS
D
| CaMK-thr305
NMDAR
| | | Same as for back_1 | | 3 |
CaMK-thr286-bind
-CaM | 1000.19
(uM^-1 s^-1) | 0.1
(s^-1) | Kd(bf) = 0.0001(uM) | - | CaMKII-thr286
CaM-Ca4
| CaMKII-thr286*-C
aM
| | | Affinity is up 1000X. Time to release is about 20 sec, so the kb is OK at 0.1 This makes Kf around 1.6666e-3 | | 4 |
CaMKII-bind-CaM | 49.9997
(uM^-1 s^-1) | 5
(s^-1) | Kd(bf) = 0.1(uM) | - | CaM-Ca4
CaMKII
| CaMKII-CaM
| | | This is tricky. There is some cooperativity here arising from interactions between the subunits of the CAMKII holoenzyme. However, the stoichiometry is 1. Kb/Kf = 6e4 #/cell. Rate is fast (see Hanson et al Neuron 12 943-956 1994) so lets say kb = 10. This gives kf = 1.6667e-4 H&S AnnRev Biochem 92 give tau for dissoc as 0.2 sec at low Ca, 0.4 at high. Low Ca = 100 nM = physiol. | | 5 |
CaMKII-bind-CaM-
PSD | 49.9998
(uM^-1 s^-1) | 0
(s^-1) | - | - | CaMKII-PSD
CaM-Ca4-PSD
| CaMKII-CaM-PSD
| | 6 |
CaMKII-diss-CaM | 5
(s^-1) | 0
(uM^-1 s^-1) | - | - | CaMKII-CaM-PSD
| CaM-Ca4-PSD
CaMKII-PSD
| | 7 |
CaMKII-thr286-bi
nd-CaM-PSD | 1000.02
(uM^-1 s^-1) | 0.1
(s^-1) | Kd(bf) = 0.0001(uM) | - | CaMKII-thr286-PS
D
CaM-Ca4-PSD
| CaMKII-thr286-Ca
M-PSD
| | | Same values as for the main compartment Can the main compartment pool of Ca/CaM be used? | | 8 |
Stoch_Basal_
CaMKII_PSD | 1
(s^-1) | 1
(s^-1) | Keq = 1(uM) | 0.5sec | basal_CaMKII_
PSD_control
| basal_CaMKII_
PSD
| | 9 |
transloc_1 | 0
(#^-1 s^-1) | 0
(s^-1) | Not applicable** | - | CaMKII-CaM
NMDAR
| CaMKII-CaM-PSD
| | | Rates to match curve in fig2 from Shen and Meyer, Science 284:162-166(1999), calculated for 6:1 alpha:beta CaMKII heterodimers | | 10 |
transloc_2 | 0
(#^-1 s^-1) | 0
(s^-1) | Not applicable** | - | CaMKII-thr286*-C
aM
NMDAR
| CaMKII-thr286-Ca
M-PSD
| | | Same as for transloc_1 |
** This is a trasport reation between compartments of different volumes. Therefore Kd is not applicable. Please Note Kf, Kb units are in number of molecules instead of concentration.
Pathway Details Molecule List Enzyme List Reaction List
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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