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Reaction List for pathway CaMKII (Pathway Number 245)

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reactions is not considered.
  Name KfKbKdtauSubstrateProduct
1 back_10.3
(s^-1)		0
(#^-1 s^-1)		Not applicable**-CaMKII-PSD
CaMKII
NMDAR
  Rates set by the translocation experiments of Shen and Meyer, Science 1999.
2 back_20.3
(s^-1)		0
(#^-1 s^-1)		Not applicable**-
  • CaMKII-thr305-PS
    D
    		• CaMK-thr305
    NMDAR
      Same as for back_1
    3 CaMK-thr286-bind
    -CaM    		1000.19
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 0.0001(uM)-CaMKII-thr286
    CaM-Ca4
  • CaMKII-thr286*-C
    aM
    •   Affinity is up 1000X. Time to release is about 20 sec, so the kb is OK at 0.1 This makes Kf around 1.6666e-3
    4 CaMKII-bind-CaM49.9997
    (uM^-1 s^-1)    		5
    (s^-1)    		Kd(bf) = 0.1(uM)-CaM-Ca4
    CaMKII
    		CaMKII-CaM
      This is tricky. There is some cooperativity here arising from interactions between the subunits of the CAMKII holoenzyme. However, the stoichiometry is 1. Kb/Kf = 6e4 #/cell. Rate is fast (see Hanson et al Neuron 12 943-956 1994) so lets say kb = 10. This gives kf = 1.6667e-4 H&S AnnRev Biochem 92 give tau for dissoc as 0.2 sec at low Ca, 0.4 at high. Low Ca = 100 nM = physiol.
    5 CaMKII-bind-CaM-
    PSD    		49.9998
    (uM^-1 s^-1)    		0
    (s^-1)    		--CaMKII-PSD
    CaM-Ca4-PSD
    		CaMKII-CaM-PSD
    6 CaMKII-diss-CaM5
    (s^-1)    		0
    (uM^-1 s^-1)    		--CaMKII-CaM-PSD
    		CaM-Ca4-PSD
    CaMKII-PSD
    7 CaMKII-thr286-bi
    nd-CaM-PSD    		1000.02
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 0.0001(uM)-
  • CaMKII-thr286-PS
    D
    CaM-Ca4-PSD
  • CaMKII-thr286-Ca
    M-PSD
    •   Same values as for the main compartment Can the main compartment pool of Ca/CaM be used?
    8 Stoch_Basal_
    CaMKII_PSD    		1
    (s^-1)    		1
    (s^-1)    		Keq = 1(uM)0.5sec
  • basal_CaMKII_
    PSD_control
  • basal_CaMKII_
    PSD
    • 9 transloc_10
    (#^-1 s^-1)    		0
    (s^-1)    		Not applicable**-CaMKII-CaM
    NMDAR
    		CaMKII-CaM-PSD
      Rates to match curve in fig2 from Shen and Meyer, Science 284:162-166(1999), calculated for 6:1 alpha:beta CaMKII heterodimers
    10 transloc_20
    (#^-1 s^-1)    		0
    (s^-1)    		Not applicable**-
  • CaMKII-thr286*-C
    aM
    NMDAR
  • CaMKII-thr286-Ca
    M-PSD
    •   Same as for transloc_1
    ** This is a trasport reation between compartments of different volumes. Therefore Kd is not applicable. Please Note Kf, Kb units are in number of molecules instead of concentration.
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