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Reaction List for pathway PKA (Pathway Number 17) in Accession fig3_CaMKII (Accession Number 2) |
Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.
Further ordering can be done to the table header.   indicates that ordering is done according to ascending or descending order.Keq is calculated only for first order reactions. Kd is calculated only for second order reactions. [nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules] |
| Reaction Name | Pathway Name / Pathway No. | Kf | Kb | Kd | tau | Reagents | |
| 1 | 1 | PKA Pathway No. 17 | 54 (uM^-1 s^-1) | 33 (s^-1) | Kd(bf) = 0.6111(uM) | - | Substrate: R2C2 cAMP Products: R2C2-cAMP | Kf = 54 /sec/uM, Kb = 33 /sec; PKA in normal human T lymphocytes. Hasler et al (1992) FASEB J 6:2735-2741 Kd =1e-07 M for type II, 5.6e-08 M for type I; Stephen B. Smith et al (1981) PNAS, USA 78: 1591-1595 Ka1 = 2.1e+07 /M which gives Kd = 47 nM, Kan = 5e+08 /M or Kd of 2nM from Fig.7 |
| 2 | 2 | PKA Pathway No. 17 | 54 (uM^-1 s^-1) | 33 (s^-1) | Kd(bf) = 0.6111(uM) | - | Substrate: R2C2-cAMP cAMP Products: R2C2-cAMP2 | Kf = 54 /sec/uM, Kb = 33 /sec; Same Km (1e-07M) assumed as site B1. kf/kb = 0.7e-07M*1e06/(6e05^02) = 2e-13:2.77e-12 |
| 3 | 1 | PKA Pathway No. 17 | 75 (uM^-1 s^-1) | 110 (s^-1) | Kd(bf) = 1.4667(uM) | - | Substrate: R2C2-cAMP2 cAMP Products: R2C2-cAMP3 | Kf = 75 /sec/uM, Kb = 110 /sec; This site has higher Kd for cAMP (kinetics within bovine myocardium) Dagfinn Ogreid and Stein Ove Doskeland (1981) FEBS Lett. 129(2):287-292 |
| 4 | 2 | PKA Pathway No. 17 | 75 (uM^-1 s^-1) | 32.5 (s^-1) | Kd(bf) = 0.4333(uM) | - | Substrate: cAMP R2C2-cAMP3 Products: R2C2-cAMP4 | Now cAMP shows effects of cooperativity and PKA has a low Kd for cAMP. |
| 5 | Release-C1 | PKA Pathway No. 17 | 60 (s^-1) | 18 (uM^-1 s^-1) | Kd(cb) = 0.3(uM) | - | Substrate: R2C2-cAMP4 Products: PKA-active R2C-cAMP4 | The complex starts to dissociate and release catalytic subunit C. This has to be fast, as the activation of PKA by cAMP is also fast. |
| 6 | Release-C2 | PKA Pathway No. 17 | 60 (s^-1) | 18 (uM^-1 s^-1) | Kd(cb) = 0.3(uM) | - | Substrate: R2C-cAMP4 Products: PKA-active R2-cAMP4 |
| 7 | inhib-PKA | PKA Pathway No. 17 | 60 (uM^-1 s^-1) | 1 (s^-1) | Kd(bf) = 0.0167(uM) | - | Substrate: PKA-active PKA-inhibitor Products: inhibited-PKA |