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Molecule Parameter List for PLA2-cytosolic

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
PLA2-cytosolic participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1001021

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Ajay_Bhalla_
    2004_Feedback_
    Tuning
  • 78Network
    Shared_Object_Ajay_Bhalla_2004_Feedback_Tuning PKC PLA2 
    PLCbeta Gq MAPK 
    Ras EGFR Sos 
    PLC_g CaMKII CaM 
    PP1 PP2B PKA 
    AC 
    This model is taken from Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80. This is the feedback model from Figure 8a.

    PLA2-cytosolic acting as a Molecule in  
    Ajay_Bhalla_2004_Feedback_Tuning Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    PLA2-cytosolic
  • Ajay_Bhalla_
    2004_Feedback_
    Tuning

    Accession No. : 78
  • PLA2
    Pathway No. : 349
    0.41.5No
    Calculated cytosolic was 20 nm from Wijkander and Sundler However, Leslie and Channon use about 400 nM. Need to confirm, but this is the value I use here. Another recalc of W&S gives 1uM

    PLA2-cytosolic acting as a Substrate for an Enzyme in  
    Ajay_Bhalla_2004_Feedback_Tuning Network
    Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2004_Feedback_
    Tuning

    Accession No. : 78
  • Shared_Object_
    Ajay_Bhalla_
    2004_Feedback_
    Tuning

    Pathway No. : 347
  • 25.6406104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
    Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5

    PLA2-cytosolic acting as a Substrate in a reaction in  
    Ajay_Bhalla_2004_Feedback_Tuning Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1PLA2-Ca-act
  • Ajay_Bhalla_
    2004_Feedback_
    Tuning

    Accession No. : 78
  • PLA2
    Pathway No. : 349
    1
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.1(uM)-Substrate
    Ca
    PLA2-cytosolic

    Product
    PLA2-Ca*
      Leslie and Channon BBA 1045 (1990) 261-270 fig6 pp267.
    2PIP2-PLA2-act
  • Ajay_Bhalla_
    2004_Feedback_
    Tuning

    Accession No. : 78
  • PLA2
    Pathway No. : 349
    0.0012
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 416.6771(uM)-Substrate
    PLA2-cytosolic
    temp-PIP2

    Product
    PIP2-PLA2*

    PLA2-cytosolic acting as a Product in a reaction in  
    Ajay_Bhalla_2004_Feedback_Tuning Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
    NameAccession NamePathway NameKfKbKdtauReagents
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2004_Feedback_
    Tuning

    Accession No. : 78
  • PLA2
    Pathway No. : 349
    0.17
    (s^-1)
    0
    (s^-1)
    --Substrate
    PLA2*

    Product
    PLA2-cytosolic



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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