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Molecule Parameter List for PPhosphatase2A

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*PPhosphatase2A* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	4	0	0	0	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Ajay_Bhalla_ 2007_Bistable	79	Network	Shared_Object_Ajay_Bhalla_2007_Bistable, PKC, PLA2, MAPK, Ras, CaM
This is a model of ERKII signaling which is bistable due to feedback. The feedback occurs through ERKII phosphorylation of phospholipase A2 (PLA2), leading to increased production of arachidonic acid (AA), which activates protein kinase C (PKC) which activates c-Raf which is upstream of ERKII. The model is a highly simplified variant of more detailed bistable models of MAPK signaling (Bhalla US, Iyengar R. Science. 1999 Jan 15;283(5400):381-7, Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80)

PPhosphatase2A acting as a Molecule in Ajay_Bhalla_2007_Bistable Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
PPhosphatase2A	Ajay_Bhalla_ 2007_Bistable Accession No. : 79	Shared_Object_ Ajay_Bhalla_ 2007_Bistable Pathway No. : 363	1	125.7	No
Refs: Pato et al Biochem J 293:35-41(93); Takai&Mieskes Biochem J 275:233-239 k1=1.46e-4, k2=1000,k3=250. these use kcat values for calponin. Also, units of kcat may be in min! revert to Vmax base: k3=6, k2=25,k1=3.3e-6 or 6,6,1e-6 CoInit assumed 0.1 uM. See NOTES for MAPK_Ras50.g. CoInit now 0.08 Sep 17 1997: Raise CoInt 1.4x to 0.224, see parm searches from jun 96 on.

PPhosphatase2A acting as an Enzyme in Ajay_Bhalla_2007_Bistable Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	PPhosphatase2A / craf-deph	Ajay_Bhalla_ 2007_Bistable Accession No. : 79	Shared_Object_ Ajay_Bhalla_ 2007_Bistable Pathway No. : 363	15.6566	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
2	PPhosphatase2A / MAPKK-deph	Ajay_Bhalla_ 2007_Bistable Accession No. : 79	Shared_Object_ Ajay_Bhalla_ 2007_Bistable Pathway No. : 363	15.6566	6	4	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
3	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_Bistable Accession No. : 79	Shared_Object_ Ajay_Bhalla_ 2007_Bistable Pathway No. : 363	15.6566	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

4	PPhosphatase2A / craf**-deph	Ajay_Bhalla_ 2007_Bistable Accession No. : 79	Shared_Object_ Ajay_Bhalla_ 2007_Bistable Pathway No. : 363	15.6566	6	4	explicit E-S complex	Substrate craf-1 Product** craf-1*
	Ueki et al JBC 269(22) pp 15756-15761 1994 show hyperphosphorylation of craf, so this is there to dephosphorylate it. Identity of phosphatase is not known to me, but it may be PP2A like the rest, so I have made it so.

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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