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Molecule Parameter List for craf-1

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
craf-1 participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences25002525250

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13
  • 82NetworkShared_Object_Ajay_Bhalla_2007_ReacDiff1_1e-13 
    PKC MAPK Ras CaM PKM chain kinetics PKC MAPK Ras CaM PKM kinetics[1] 
    PKC MAPK Ras CaM PKM kinetics[2] PKC MAPK Ras CaM PKM kinetics[3] 
    PKC MAPK Ras CaM PKM kinetics[4] PKC MAPK Ras CaM PKM kinetics[5] 
    PKC MAPK Ras kinetics[6] CaM PKM PKC MAPK Ras CaM PKM kinetics[7] 
    PKC MAPK Ras CaM PKM kinetics[8] PKC MAPK Ras CaM PKM kinetics[9] 
    PKC MAPK Ras CaM PKM kinetics[10] PKC MAPK Ras CaM PKM kinetics[11] 
    PKC MAPK Ras CaM PKM kinetics[12] PKC MAPK Ras CaM PKM kinetics[13] 
    PKC MAPK Ras CaM PKM kinetics[14] PKC MAPK Ras CaM PKM kinetics[15] 
    PKC MAPK Ras CaM PKM kinetics[16] PKC MAPK Ras CaM PKM kinetics[17] 
    PKC MAPK Ras CaM PKM kinetics[18] PKC MAPK Ras CaM PKM kinetics[19] 
    PKC MAPK Ras CaM PKM kinetics[20] PKC MAPK Ras CaM PKM kinetics[21] 
    PKC MAPK Ras CaM PKM kinetics[22] PKC MAPK Ras CaM PKM kinetics[23] 
    PKC MAPK Ras CaM PKM 
    This is a 25-compartment reaction-diffusion version of the Ajay_Bhalla_2007_PKM model. The original single-compartment model is repeated 25 times. In addition, a subset (27 out of 42) molecules can diffuse between compartments. Diffusion is implemented as a reaction between corresponding molecules in neighboring compartments. For D = 1e-12 m^2/sec (i.e., 1 micron^2/sec ) the kf and kb of this reaction for these 10 micron compartments are both 0.01/sec. For D = 1e-13 m^2/sec (i.e., 0.1 micron^2/sec ) the kf and kb are 0.001/sec.
    The stimulus file pkm_mapk22_diff_1e-13_Fig4B which was used for the model to replicate Figure 4B from the paper.
    pkm_mapk22_diff_1e-13_Fig4H replicate Figure 4H.
    pkm_mapk22_diff_1e-13_Fig4I replicate Figure 4I.

    craf-1 acting as a Molecule in  
    Ajay_Bhalla_2007_ReacDiff1_1e-13 Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    craf-1
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • MAPK
    Pathway No. : 528
    0.51.5No
    Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
    craf-1
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • MAPK
    Pathway No. : 535
    0.51.5No
    Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
    craf-1
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • MAPK
    Pathway No. : 541
    0.51.5No
    Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
    craf-1
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • MAPK
    Pathway No. : 547
    0.51.5No
    Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
    craf-1
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • MAPK
    Pathway No. : 553
    0.51.5No
    Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
    craf-1
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • MAPK
    Pathway No. : 559
    0.51.5No
    Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
    craf-1
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • MAPK
    Pathway No. : 565
    0.51.5No
    Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
    craf-1
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • MAPK
    Pathway No. : 571
    0.51.5No
    Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
    craf-1
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • MAPK
    Pathway No. : 577
    0.51.5No
    Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
    craf-1
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • MAPK
    Pathway No. : 583
    0.51.5No
    Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
    craf-1
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • MAPK
    Pathway No. : 589
    0.51.5No
    Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
    craf-1
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • MAPK
    Pathway No. : 595
    0.51.5No
    Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
    craf-1
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • MAPK
    Pathway No. : 601
    0.51.5No
    Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
    craf-1
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • MAPK
    Pathway No. : 607
    0.51.5No
    Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
    craf-1
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • MAPK
    Pathway No. : 613
    0.51.5No
    Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
    craf-1
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • MAPK
    Pathway No. : 619
    0.51.5No
    Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
    craf-1
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • MAPK
    Pathway No. : 625
    0.51.5No
    Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
    craf-1
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • MAPK
    Pathway No. : 631
    0.51.5No
    Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
    craf-1
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • MAPK
    Pathway No. : 637
    0.51.5No
    Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
    craf-1
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • MAPK
    Pathway No. : 643
    0.51.5No
    Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
    craf-1
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • MAPK
    Pathway No. : 649
    0.51.5No
    Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
    craf-1
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • MAPK
    Pathway No. : 655
    0.51.5No
    Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
    craf-1
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • MAPK
    Pathway No. : 661
    0.51.5No
    Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
    craf-1
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • MAPK
    Pathway No. : 667
    0.51.5No
    Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
    craf-1
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • MAPK
    Pathway No. : 673
    0.51.5No
    Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006

    craf-1 acting as a Substrate for an Enzyme in  
    Ajay_Bhalla_2007_ReacDiff1_1e-13 Network
     Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    1PKC-active  /
    PKC-act-raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Pathway No. : 526
  • 20.000544explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
        Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
    2PKC-active  /
    PKC-act-raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics
    Pathway No. : 533
    20.000544explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
        Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
    3PKC-active  /
    PKC-act-raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[1]
    Pathway No. : 539
    20.000544explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
        Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
    4PKC-active  /
    PKC-act-raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[2]
    Pathway No. : 545
    20.000544explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
        Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
    5PKC-active  /
    PKC-act-raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[3]
    Pathway No. : 551
    20.000544explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
        Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
    6PKC-active  /
    PKC-act-raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[4]
    Pathway No. : 557
    20.000544explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
        Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
    7PKC-active  /
    PKC-act-raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[5]
    Pathway No. : 563
    20.000544explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
        Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
    8PKC-active  /
    PKC-act-raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[6]
    Pathway No. : 567
    20.000544explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
        Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
    9PKC-active  /
    PKC-act-raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[7]
    Pathway No. : 575
    20.000544explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
        Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
    10PKC-active  /
    PKC-act-raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[8]
    Pathway No. : 581
    20.000544explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
        Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
    11PKC-active  /
    PKC-act-raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[9]
    Pathway No. : 587
    20.000544explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
        Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
    12PKC-active  /
    PKC-act-raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[10]
    Pathway No. : 593
    20.000544explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
        Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
    13PKC-active  /
    PKC-act-raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[11]
    Pathway No. : 599
    20.000544explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
        Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
    14PKC-active  /
    PKC-act-raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[12]
    Pathway No. : 605
    20.000544explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
        Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
    15PKC-active  /
    PKC-act-raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[13]
    Pathway No. : 611
    20.000544explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
        Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
    16PKC-active  /
    PKC-act-raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[14]
    Pathway No. : 617
    20.000544explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
        Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
    17PKC-active  /
    PKC-act-raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[15]
    Pathway No. : 623
    20.000544explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
        Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
    18PKC-active  /
    PKC-act-raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[16]
    Pathway No. : 629
    20.000544explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
        Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
    19PKC-active  /
    PKC-act-raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[17]
    Pathway No. : 635
    20.000544explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
        Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
    20PKC-active  /
    PKC-act-raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[18]
    Pathway No. : 641
    20.000544explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
        Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
    21PKC-active  /
    PKC-act-raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[19]
    Pathway No. : 647
    20.000544explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
        Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
    22PKC-active  /
    PKC-act-raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[20]
    Pathway No. : 653
    20.000544explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
        Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
    23PKC-active  /
    PKC-act-raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[21]
    Pathway No. : 659
    20.000544explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
        Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
    24PKC-active  /
    PKC-act-raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[22]
    Pathway No. : 665
    20.000544explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
        Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
    25PKC-active  /
    PKC-act-raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[23]
    Pathway No. : 671
    20.000544explicit E-S complexSubstrate
    craf-1

    Product
    craf-1*
        Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC

    craf-1 acting as a Product of an Enzyme in  
    Ajay_Bhalla_2007_ReacDiff1_1e-13 Network
     Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    1PPhosphatase2A  /
    craf-deph
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Pathway No. : 526
  • 15.656764explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
        See parent PPhosphatase2A for parms
    2PPhosphatase2A  /
    craf-deph
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics
    Pathway No. : 533
    15.656764explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
        See parent PPhosphatase2A for parms
    3PPhosphatase2A  /
    craf-deph
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[1]
    Pathway No. : 539
    15.656764explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
        See parent PPhosphatase2A for parms
    4PPhosphatase2A  /
    craf-deph
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[2]
    Pathway No. : 545
    15.656764explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
        See parent PPhosphatase2A for parms
    5PPhosphatase2A  /
    craf-deph
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[3]
    Pathway No. : 551
    15.656764explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
        See parent PPhosphatase2A for parms
    6PPhosphatase2A  /
    craf-deph
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[4]
    Pathway No. : 557
    15.656764explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
        See parent PPhosphatase2A for parms
    7PPhosphatase2A  /
    craf-deph
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[5]
    Pathway No. : 563
    15.656764explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
        See parent PPhosphatase2A for parms
    8PPhosphatase2A  /
    craf-deph
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[6]
    Pathway No. : 567
    15.656764explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
        See parent PPhosphatase2A for parms
    9PPhosphatase2A  /
    craf-deph
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[7]
    Pathway No. : 575
    15.656764explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
        See parent PPhosphatase2A for parms
    10PPhosphatase2A  /
    craf-deph
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[8]
    Pathway No. : 581
    15.656764explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
        See parent PPhosphatase2A for parms
    11PPhosphatase2A  /
    craf-deph
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[9]
    Pathway No. : 587
    15.656764explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
        See parent PPhosphatase2A for parms
    12PPhosphatase2A  /
    craf-deph
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[10]
    Pathway No. : 593
    15.656764explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
        See parent PPhosphatase2A for parms
    13PPhosphatase2A  /
    craf-deph
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[11]
    Pathway No. : 599
    15.656764explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
        See parent PPhosphatase2A for parms
    14PPhosphatase2A  /
    craf-deph
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[12]
    Pathway No. : 605
    15.656764explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
        See parent PPhosphatase2A for parms
    15PPhosphatase2A  /
    craf-deph
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[13]
    Pathway No. : 611
    15.656764explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
        See parent PPhosphatase2A for parms
    16PPhosphatase2A  /
    craf-deph
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[14]
    Pathway No. : 617
    15.656764explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
        See parent PPhosphatase2A for parms
    17PPhosphatase2A  /
    craf-deph
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[15]
    Pathway No. : 623
    15.656764explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
        See parent PPhosphatase2A for parms
    18PPhosphatase2A  /
    craf-deph
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[16]
    Pathway No. : 629
    15.656764explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
        See parent PPhosphatase2A for parms
    19PPhosphatase2A  /
    craf-deph
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[17]
    Pathway No. : 635
    15.656764explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
        See parent PPhosphatase2A for parms
    20PPhosphatase2A  /
    craf-deph
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[18]
    Pathway No. : 641
    15.656764explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
        See parent PPhosphatase2A for parms
    21PPhosphatase2A  /
    craf-deph
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[19]
    Pathway No. : 647
    15.656764explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
        See parent PPhosphatase2A for parms
    22PPhosphatase2A  /
    craf-deph
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[20]
    Pathway No. : 653
    15.656764explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
        See parent PPhosphatase2A for parms
    23PPhosphatase2A  /
    craf-deph
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[21]
    Pathway No. : 659
    15.656764explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
        See parent PPhosphatase2A for parms
    24PPhosphatase2A  /
    craf-deph
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[22]
    Pathway No. : 665
    15.656764explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
        See parent PPhosphatase2A for parms
    25PPhosphatase2A  /
    craf-deph
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[23]
    Pathway No. : 671
    15.656764explicit E-S complexSubstrate
    craf-1*

    Product
    craf-1
        See parent PPhosphatase2A for parms

    craf-1 acting as a Substrate in a reaction in  
    Ajay_Bhalla_2007_ReacDiff1_1e-13 Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1
  • Ras-act-unphosph
    -raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Pathway No. : 526
  • 0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    2
  • Ras-act-unphosph
    -raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics
    Pathway No. : 533
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    3
  • Ras-act-unphosph
    -raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[1]
    Pathway No. : 539
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    4
  • Ras-act-unphosph
    -raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[2]
    Pathway No. : 545
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    5
  • Ras-act-unphosph
    -raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[3]
    Pathway No. : 551
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    6
  • Ras-act-unphosph
    -raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[4]
    Pathway No. : 557
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    7
  • Ras-act-unphosph
    -raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[5]
    Pathway No. : 563
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    8
  • Ras-act-unphosph
    -raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[6]
    Pathway No. : 567
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    9
  • Ras-act-unphosph
    -raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[7]
    Pathway No. : 575
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    10
  • Ras-act-unphosph
    -raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[8]
    Pathway No. : 581
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    11
  • Ras-act-unphosph
    -raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[9]
    Pathway No. : 587
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    12
  • Ras-act-unphosph
    -raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[10]
    Pathway No. : 593
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    13
  • Ras-act-unphosph
    -raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[11]
    Pathway No. : 599
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    14
  • Ras-act-unphosph
    -raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[12]
    Pathway No. : 605
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    15
  • Ras-act-unphosph
    -raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[13]
    Pathway No. : 611
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    16
  • Ras-act-unphosph
    -raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[14]
    Pathway No. : 617
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    17
  • Ras-act-unphosph
    -raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[15]
    Pathway No. : 623
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    18
  • Ras-act-unphosph
    -raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[16]
    Pathway No. : 629
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    19
  • Ras-act-unphosph
    -raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[17]
    Pathway No. : 635
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    20
  • Ras-act-unphosph
    -raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[18]
    Pathway No. : 641
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    21
  • Ras-act-unphosph
    -raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[19]
    Pathway No. : 647
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    22
  • Ras-act-unphosph
    -raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[20]
    Pathway No. : 653
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    23
  • Ras-act-unphosph
    -raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[21]
    Pathway No. : 659
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    24
  • Ras-act-unphosph
    -raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[22]
    Pathway No. : 665
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
    25
  • Ras-act-unphosph
    -raf
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • kinetics[23]
    Pathway No. : 671
    0
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras
    craf-1

    Product
    Raf-GTP-Ras
      18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.



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