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Molecule Parameter List for GDP-Ras

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
GDP-Ras participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences25007525025

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13
  • 82NetworkShared_Object_Ajay_Bhalla_2007_ReacDiff1_1e-13 
    PKC MAPK Ras CaM PKM chain kinetics PKC MAPK Ras CaM PKM kinetics[1] 
    PKC MAPK Ras CaM PKM kinetics[2] PKC MAPK Ras CaM PKM kinetics[3] 
    PKC MAPK Ras CaM PKM kinetics[4] PKC MAPK Ras CaM PKM kinetics[5] 
    PKC MAPK Ras kinetics[6] CaM PKM PKC MAPK Ras CaM PKM kinetics[7] 
    PKC MAPK Ras CaM PKM kinetics[8] PKC MAPK Ras CaM PKM kinetics[9] 
    PKC MAPK Ras CaM PKM kinetics[10] PKC MAPK Ras CaM PKM kinetics[11] 
    PKC MAPK Ras CaM PKM kinetics[12] PKC MAPK Ras CaM PKM kinetics[13] 
    PKC MAPK Ras CaM PKM kinetics[14] PKC MAPK Ras CaM PKM kinetics[15] 
    PKC MAPK Ras CaM PKM kinetics[16] PKC MAPK Ras CaM PKM kinetics[17] 
    PKC MAPK Ras CaM PKM kinetics[18] PKC MAPK Ras CaM PKM kinetics[19] 
    PKC MAPK Ras CaM PKM kinetics[20] PKC MAPK Ras CaM PKM kinetics[21] 
    PKC MAPK Ras CaM PKM kinetics[22] PKC MAPK Ras CaM PKM kinetics[23] 
    PKC MAPK Ras CaM PKM 
    This is a 25-compartment reaction-diffusion version of the Ajay_Bhalla_2007_PKM model. The original single-compartment model is repeated 25 times. In addition, a subset (27 out of 42) molecules can diffuse between compartments. Diffusion is implemented as a reaction between corresponding molecules in neighboring compartments. For D = 1e-12 m^2/sec (i.e., 1 micron^2/sec ) the kf and kb of this reaction for these 10 micron compartments are both 0.01/sec. For D = 1e-13 m^2/sec (i.e., 0.1 micron^2/sec ) the kf and kb are 0.001/sec.
    The stimulus file pkm_mapk22_diff_1e-13_Fig4B which was used for the model to replicate Figure 4B from the paper.
    pkm_mapk22_diff_1e-13_Fig4H replicate Figure 4H.
    pkm_mapk22_diff_1e-13_Fig4I replicate Figure 4I.

    GDP-Ras acting as a Molecule in  
    Ajay_Bhalla_2007_ReacDiff1_1e-13 Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    GDP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 529
    0.51.5No
    GDP bound form. See Rosen et al Neuron 12 1207-1221 June 1994. the activation loop is based on Boguski and McCormick Nature 366 643-654 93 Assume Ras is present at about the same level as craf-1, 0.2 uM. Hallberg et al JBC 269:6 3913-3916 1994 estimate upto 5-10% of cellular Raf is assoc with Ras. Given that only 5-10% of Ras is GTP-bound, we need similar amounts of Ras as Raf.
    GDP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 536
    0.51.5No
    GDP bound form. See Rosen et al Neuron 12 1207-1221 June 1994. the activation loop is based on Boguski and McCormick Nature 366 643-654 93 Assume Ras is present at about the same level as craf-1, 0.2 uM. Hallberg et al JBC 269:6 3913-3916 1994 estimate upto 5-10% of cellular Raf is assoc with Ras. Given that only 5-10% of Ras is GTP-bound, we need similar amounts of Ras as Raf.
    GDP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 542
    0.51.5No
    GDP bound form. See Rosen et al Neuron 12 1207-1221 June 1994. the activation loop is based on Boguski and McCormick Nature 366 643-654 93 Assume Ras is present at about the same level as craf-1, 0.2 uM. Hallberg et al JBC 269:6 3913-3916 1994 estimate upto 5-10% of cellular Raf is assoc with Ras. Given that only 5-10% of Ras is GTP-bound, we need similar amounts of Ras as Raf.
    GDP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 548
    0.51.5No
    GDP bound form. See Rosen et al Neuron 12 1207-1221 June 1994. the activation loop is based on Boguski and McCormick Nature 366 643-654 93 Assume Ras is present at about the same level as craf-1, 0.2 uM. Hallberg et al JBC 269:6 3913-3916 1994 estimate upto 5-10% of cellular Raf is assoc with Ras. Given that only 5-10% of Ras is GTP-bound, we need similar amounts of Ras as Raf.
    GDP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 554
    0.51.5No
    GDP bound form. See Rosen et al Neuron 12 1207-1221 June 1994. the activation loop is based on Boguski and McCormick Nature 366 643-654 93 Assume Ras is present at about the same level as craf-1, 0.2 uM. Hallberg et al JBC 269:6 3913-3916 1994 estimate upto 5-10% of cellular Raf is assoc with Ras. Given that only 5-10% of Ras is GTP-bound, we need similar amounts of Ras as Raf.
    GDP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 560
    0.51.5No
    GDP bound form. See Rosen et al Neuron 12 1207-1221 June 1994. the activation loop is based on Boguski and McCormick Nature 366 643-654 93 Assume Ras is present at about the same level as craf-1, 0.2 uM. Hallberg et al JBC 269:6 3913-3916 1994 estimate upto 5-10% of cellular Raf is assoc with Ras. Given that only 5-10% of Ras is GTP-bound, we need similar amounts of Ras as Raf.
    GDP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 566
    0.51.5No
    GDP bound form. See Rosen et al Neuron 12 1207-1221 June 1994. the activation loop is based on Boguski and McCormick Nature 366 643-654 93 Assume Ras is present at about the same level as craf-1, 0.2 uM. Hallberg et al JBC 269:6 3913-3916 1994 estimate upto 5-10% of cellular Raf is assoc with Ras. Given that only 5-10% of Ras is GTP-bound, we need similar amounts of Ras as Raf.
    GDP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 572
    0.51.5No
    GDP bound form. See Rosen et al Neuron 12 1207-1221 June 1994. the activation loop is based on Boguski and McCormick Nature 366 643-654 93 Assume Ras is present at about the same level as craf-1, 0.2 uM. Hallberg et al JBC 269:6 3913-3916 1994 estimate upto 5-10% of cellular Raf is assoc with Ras. Given that only 5-10% of Ras is GTP-bound, we need similar amounts of Ras as Raf.
    GDP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 578
    0.51.5No
    GDP bound form. See Rosen et al Neuron 12 1207-1221 June 1994. the activation loop is based on Boguski and McCormick Nature 366 643-654 93 Assume Ras is present at about the same level as craf-1, 0.2 uM. Hallberg et al JBC 269:6 3913-3916 1994 estimate upto 5-10% of cellular Raf is assoc with Ras. Given that only 5-10% of Ras is GTP-bound, we need similar amounts of Ras as Raf.
    GDP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 584
    0.51.5No
    GDP bound form. See Rosen et al Neuron 12 1207-1221 June 1994. the activation loop is based on Boguski and McCormick Nature 366 643-654 93 Assume Ras is present at about the same level as craf-1, 0.2 uM. Hallberg et al JBC 269:6 3913-3916 1994 estimate upto 5-10% of cellular Raf is assoc with Ras. Given that only 5-10% of Ras is GTP-bound, we need similar amounts of Ras as Raf.
    GDP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 590
    0.51.5No
    GDP bound form. See Rosen et al Neuron 12 1207-1221 June 1994. the activation loop is based on Boguski and McCormick Nature 366 643-654 93 Assume Ras is present at about the same level as craf-1, 0.2 uM. Hallberg et al JBC 269:6 3913-3916 1994 estimate upto 5-10% of cellular Raf is assoc with Ras. Given that only 5-10% of Ras is GTP-bound, we need similar amounts of Ras as Raf.
    GDP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 596
    0.51.5No
    GDP bound form. See Rosen et al Neuron 12 1207-1221 June 1994. the activation loop is based on Boguski and McCormick Nature 366 643-654 93 Assume Ras is present at about the same level as craf-1, 0.2 uM. Hallberg et al JBC 269:6 3913-3916 1994 estimate upto 5-10% of cellular Raf is assoc with Ras. Given that only 5-10% of Ras is GTP-bound, we need similar amounts of Ras as Raf.
    GDP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 602
    0.51.5No
    GDP bound form. See Rosen et al Neuron 12 1207-1221 June 1994. the activation loop is based on Boguski and McCormick Nature 366 643-654 93 Assume Ras is present at about the same level as craf-1, 0.2 uM. Hallberg et al JBC 269:6 3913-3916 1994 estimate upto 5-10% of cellular Raf is assoc with Ras. Given that only 5-10% of Ras is GTP-bound, we need similar amounts of Ras as Raf.
    GDP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 608
    0.51.5No
    GDP bound form. See Rosen et al Neuron 12 1207-1221 June 1994. the activation loop is based on Boguski and McCormick Nature 366 643-654 93 Assume Ras is present at about the same level as craf-1, 0.2 uM. Hallberg et al JBC 269:6 3913-3916 1994 estimate upto 5-10% of cellular Raf is assoc with Ras. Given that only 5-10% of Ras is GTP-bound, we need similar amounts of Ras as Raf.
    GDP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 614
    0.51.5No
    GDP bound form. See Rosen et al Neuron 12 1207-1221 June 1994. the activation loop is based on Boguski and McCormick Nature 366 643-654 93 Assume Ras is present at about the same level as craf-1, 0.2 uM. Hallberg et al JBC 269:6 3913-3916 1994 estimate upto 5-10% of cellular Raf is assoc with Ras. Given that only 5-10% of Ras is GTP-bound, we need similar amounts of Ras as Raf.
    GDP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 620
    0.51.5No
    GDP bound form. See Rosen et al Neuron 12 1207-1221 June 1994. the activation loop is based on Boguski and McCormick Nature 366 643-654 93 Assume Ras is present at about the same level as craf-1, 0.2 uM. Hallberg et al JBC 269:6 3913-3916 1994 estimate upto 5-10% of cellular Raf is assoc with Ras. Given that only 5-10% of Ras is GTP-bound, we need similar amounts of Ras as Raf.
    GDP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 626
    0.51.5No
    GDP bound form. See Rosen et al Neuron 12 1207-1221 June 1994. the activation loop is based on Boguski and McCormick Nature 366 643-654 93 Assume Ras is present at about the same level as craf-1, 0.2 uM. Hallberg et al JBC 269:6 3913-3916 1994 estimate upto 5-10% of cellular Raf is assoc with Ras. Given that only 5-10% of Ras is GTP-bound, we need similar amounts of Ras as Raf.
    GDP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 632
    0.51.5No
    GDP bound form. See Rosen et al Neuron 12 1207-1221 June 1994. the activation loop is based on Boguski and McCormick Nature 366 643-654 93 Assume Ras is present at about the same level as craf-1, 0.2 uM. Hallberg et al JBC 269:6 3913-3916 1994 estimate upto 5-10% of cellular Raf is assoc with Ras. Given that only 5-10% of Ras is GTP-bound, we need similar amounts of Ras as Raf.
    GDP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 638
    0.51.5No
    GDP bound form. See Rosen et al Neuron 12 1207-1221 June 1994. the activation loop is based on Boguski and McCormick Nature 366 643-654 93 Assume Ras is present at about the same level as craf-1, 0.2 uM. Hallberg et al JBC 269:6 3913-3916 1994 estimate upto 5-10% of cellular Raf is assoc with Ras. Given that only 5-10% of Ras is GTP-bound, we need similar amounts of Ras as Raf.
    GDP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 644
    0.51.5No
    GDP bound form. See Rosen et al Neuron 12 1207-1221 June 1994. the activation loop is based on Boguski and McCormick Nature 366 643-654 93 Assume Ras is present at about the same level as craf-1, 0.2 uM. Hallberg et al JBC 269:6 3913-3916 1994 estimate upto 5-10% of cellular Raf is assoc with Ras. Given that only 5-10% of Ras is GTP-bound, we need similar amounts of Ras as Raf.
    GDP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 650
    0.51.5No
    GDP bound form. See Rosen et al Neuron 12 1207-1221 June 1994. the activation loop is based on Boguski and McCormick Nature 366 643-654 93 Assume Ras is present at about the same level as craf-1, 0.2 uM. Hallberg et al JBC 269:6 3913-3916 1994 estimate upto 5-10% of cellular Raf is assoc with Ras. Given that only 5-10% of Ras is GTP-bound, we need similar amounts of Ras as Raf.
    GDP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 656
    0.51.5No
    GDP bound form. See Rosen et al Neuron 12 1207-1221 June 1994. the activation loop is based on Boguski and McCormick Nature 366 643-654 93 Assume Ras is present at about the same level as craf-1, 0.2 uM. Hallberg et al JBC 269:6 3913-3916 1994 estimate upto 5-10% of cellular Raf is assoc with Ras. Given that only 5-10% of Ras is GTP-bound, we need similar amounts of Ras as Raf.
    GDP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 662
    0.51.5No
    GDP bound form. See Rosen et al Neuron 12 1207-1221 June 1994. the activation loop is based on Boguski and McCormick Nature 366 643-654 93 Assume Ras is present at about the same level as craf-1, 0.2 uM. Hallberg et al JBC 269:6 3913-3916 1994 estimate upto 5-10% of cellular Raf is assoc with Ras. Given that only 5-10% of Ras is GTP-bound, we need similar amounts of Ras as Raf.
    GDP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 668
    0.51.5No
    GDP bound form. See Rosen et al Neuron 12 1207-1221 June 1994. the activation loop is based on Boguski and McCormick Nature 366 643-654 93 Assume Ras is present at about the same level as craf-1, 0.2 uM. Hallberg et al JBC 269:6 3913-3916 1994 estimate upto 5-10% of cellular Raf is assoc with Ras. Given that only 5-10% of Ras is GTP-bound, we need similar amounts of Ras as Raf.
    GDP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 674
    0.51.5No
    GDP bound form. See Rosen et al Neuron 12 1207-1221 June 1994. the activation loop is based on Boguski and McCormick Nature 366 643-654 93 Assume Ras is present at about the same level as craf-1, 0.2 uM. Hallberg et al JBC 269:6 3913-3916 1994 estimate upto 5-10% of cellular Raf is assoc with Ras. Given that only 5-10% of Ras is GTP-bound, we need similar amounts of Ras as Raf.

    GDP-Ras acting as a Substrate for an Enzyme in  
    Ajay_Bhalla_2007_ReacDiff1_1e-13 Network
     Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    1inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 529
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    2GEF*  /
    GEF*-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 529
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    3CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 529
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    4inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 536
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    5GEF*  /
    GEF*-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 536
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    6CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 536
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    7inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 542
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    8GEF*  /
    GEF*-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 542
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    9CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 542
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    10inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 548
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    11GEF*  /
    GEF*-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 548
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    12CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 548
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    13inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 554
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    14GEF*  /
    GEF*-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 554
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    15CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 554
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    16inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 560
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    17GEF*  /
    GEF*-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 560
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    18CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 560
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    19inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 566
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    20GEF*  /
    GEF*-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 566
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    21CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 566
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    22inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 572
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    23GEF*  /
    GEF*-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 572
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    24CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 572
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    25inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 578
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    26GEF*  /
    GEF*-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 578
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    27CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 578
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    28inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 584
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    29GEF*  /
    GEF*-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 584
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    30CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 584
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    31inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 590
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    32GEF*  /
    GEF*-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 590
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    33CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 590
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    34inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 596
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    35GEF*  /
    GEF*-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 596
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    36CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 596
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    37inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 602
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    38GEF*  /
    GEF*-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 602
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    39CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 602
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    40inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 608
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    41GEF*  /
    GEF*-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 608
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    42CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 608
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    43inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 614
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    44GEF*  /
    GEF*-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 614
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    45CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 614
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    46inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 620
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    47GEF*  /
    GEF*-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 620
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    48CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 620
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    49inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 626
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    50GEF*  /
    GEF*-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 626
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    51CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 626
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    52inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 632
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    53GEF*  /
    GEF*-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 632
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    54CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 632
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    55inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 638
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    56GEF*  /
    GEF*-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 638
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    57CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 638
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    58inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 644
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    59GEF*  /
    GEF*-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 644
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    60CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 644
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    61inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 650
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    62GEF*  /
    GEF*-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 650
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    63CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 650
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    64inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 656
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    65GEF*  /
    GEF*-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 656
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    66CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 656
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    67inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 662
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    68GEF*  /
    GEF*-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 662
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    69CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 662
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    70inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 668
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    71GEF*  /
    GEF*-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 668
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    72CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 668
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
    73inact-GEF  /
  • basal_GEF_
    activity
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 674
    10.10150.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
       
    74GEF*  /
    GEF*-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 674
    0.5050570.024explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg-act-ras
    75CaM-GEF  /
    CaM-GEF-act-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 674
    0.5050570.24explicit E-S complexSubstrate
    GDP-Ras

    Product
    GTP-Ras
        Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.

    GDP-Ras acting as a Product of an Enzyme in  
    Ajay_Bhalla_2007_ReacDiff1_1e-13 Network
     Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    1GAP  /
    GAP-inact-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 529
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    2GAP  /
    GAP-inact-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 536
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    3GAP  /
    GAP-inact-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 542
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    4GAP  /
    GAP-inact-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 548
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    5GAP  /
    GAP-inact-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 554
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    6GAP  /
    GAP-inact-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 560
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    7GAP  /
    GAP-inact-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 566
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    8GAP  /
    GAP-inact-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 572
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    9GAP  /
    GAP-inact-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 578
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    10GAP  /
    GAP-inact-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 584
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    11GAP  /
    GAP-inact-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 590
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    12GAP  /
    GAP-inact-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 596
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    13GAP  /
    GAP-inact-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 602
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    14GAP  /
    GAP-inact-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 608
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    15GAP  /
    GAP-inact-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 614
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    16GAP  /
    GAP-inact-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 620
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    17GAP  /
    GAP-inact-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 626
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    18GAP  /
    GAP-inact-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 632
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    19GAP  /
    GAP-inact-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 638
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    20GAP  /
    GAP-inact-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 644
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    21GAP  /
    GAP-inact-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 650
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    22GAP  /
    GAP-inact-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 656
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    23GAP  /
    GAP-inact-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 662
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    24GAP  /
    GAP-inact-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 668
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    25GAP  /
    GAP-inact-ras
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 674
    1.0104104explicit E-S complexSubstrate
    GTP-Ras

    Product
    GDP-Ras
        From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.

    GDP-Ras acting as a Product in a reaction in  
    Ajay_Bhalla_2007_ReacDiff1_1e-13 Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1
  • Ras-intrinsic-GT
    Pase
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 529
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    2
  • Ras-intrinsic-GT
    Pase
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 536
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    3
  • Ras-intrinsic-GT
    Pase
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 542
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    4
  • Ras-intrinsic-GT
    Pase
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 548
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    5
  • Ras-intrinsic-GT
    Pase
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 554
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    6
  • Ras-intrinsic-GT
    Pase
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 560
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    7
  • Ras-intrinsic-GT
    Pase
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 566
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    8
  • Ras-intrinsic-GT
    Pase
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 572
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    9
  • Ras-intrinsic-GT
    Pase
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 578
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    10
  • Ras-intrinsic-GT
    Pase
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 584
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    11
  • Ras-intrinsic-GT
    Pase
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 590
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    12
  • Ras-intrinsic-GT
    Pase
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 596
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    13
  • Ras-intrinsic-GT
    Pase
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 602
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    14
  • Ras-intrinsic-GT
    Pase
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 608
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    15
  • Ras-intrinsic-GT
    Pase
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 614
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    16
  • Ras-intrinsic-GT
    Pase
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 620
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    17
  • Ras-intrinsic-GT
    Pase
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 626
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    18
  • Ras-intrinsic-GT
    Pase
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 632
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    19
  • Ras-intrinsic-GT
    Pase
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 638
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    20
  • Ras-intrinsic-GT
    Pase
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 644
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    21
  • Ras-intrinsic-GT
    Pase
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 650
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    22
  • Ras-intrinsic-GT
    Pase
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 656
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    23
  • Ras-intrinsic-GT
    Pase
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 662
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    24
  • Ras-intrinsic-GT
    Pase
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 668
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    25
  • Ras-intrinsic-GT
    Pase
  • Ajay_Bhalla_
    2007_ReacDiff1_
    1e-13

    Accession No. : 82
  • Ras
    Pathway No. : 674
    0.0001
    (s^-1)
    0
    (s^-1)
    --Substrate
    GTP-Ras

    Product
    GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4



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