| |
Name | Pathway Name /
Pathway No. | Accession
Type | Initial
Conc.
(uM) | Volume
(fL) | Buffered | Sum Total Of |
| 1 | R2C2 | PKA
Pathway No. 270 | Network | 0.5 | 0.09 | No | - |
| | This is the R2C2 complex, consisting of 2 catalytic (C) subunits, and the R-dimer. See Taylor et al Ann Rev Biochem 1990 59:971-1005 for a review. The Doskeland and Ogreid review is better for numbers. Amount of PKA is about .5 uM. |
| 2 | R2C2-cAMP | PKA
Pathway No. 270 | Network | 0 | 0.09 | No | - |
| | CoInit was .0624 |
| 3 | R2C2-cAMP2 | PKA
Pathway No. 270 | Network | 0 | 0.09 | No | - |
| 4 | R2C2-cAMP3 | PKA
Pathway No. 270 | Network | 0 | 0.09 | No | - |
| 5 | R2C2-cAMP4 | PKA
Pathway No. 270 | Network | 0 | 0.09 | No | - |
| 6 | R2C-cAMP4 | PKA
Pathway No. 270 | Network | 0 | 0.09 | No | - |
| 7 | R2-cAMP4 | PKA
Pathway No. 270 | Network | 0 | 0.09 | No | - |
| | Starts at 0.15 for the test of fig 6 in Smith et al, but we aren't using that paper any more. |
| 8 | PKA-inhibitor | PKA
Pathway No. 270 | Network | 0.2593 | 0.09 | No | - |
| | About 25% of PKA C subunit is dissociated in resting cells without having any noticable activity. Doskeland and Ogreid Int J biochem 13 pp1-19 suggest that this is because there is a corresponding amount of inhibitor protein. |
| 9 | inhibited-PKA | PKA
Pathway No. 270 | Network | 0 | 0.09 | No | - |
| 10 | AC1-CaM | AC
Pathway No. 269 | Network | 0 | 0.09 | No | - |
| | This version of cyclase is Calmodulin activated. Gs stims it but betagamma inhibits. |
| 11 | AC1 | AC
Pathway No. 269 | Network | 0.0741 | 0.09 | No | - |
| | Starting conc at 20 nM. Reduced conc to 10 nM, 14.8. Arnold 17 Feb 2005. Raised conc back to about 18 nM, for an nInit of precisely 1. 18 Feb. Doubled conc, to compensate for reduced ATP. 18 Feb. Doublec conc again, halved Vmax. |
| 12 | AC2* | AC
Pathway No. 269 | Network | 0 | 0.09 | No | - |
| | This version is activated by Gs and by a betagamma and phosphorylation. |
| 13 | AC2 | AC
Pathway No. 269 | Network | 0.0741 | 0.09 | No | - |
| | Starting at 0.015 uM. 17 Feb 2005: Raised slightly so as to have integer number of molecules. 18 Feb 2005: Doubled, to compensate for reduced ATP. |
| 14 | AMP | AC
Pathway No. 269 | Network | 0 | 0.09 | No | - |
| 15 | cAMP-PDE | AC
Pathway No. 269 | Network | 0.5556 | 0.09 | No | - |
| | The levels of the PDE are not known at this time. However, enough kinetic info and info about steady-state levels of cAMP etc are around to make it possible to estimate this. 18 Feb 2005: After some playing with initial conc, it is now back at 0.5 uM. |
| 16 | cAMP-PDE* | AC
Pathway No. 269 | Network | 0 | 0.09 | No | - |
| | This form has about 2X activity as plain PDE. See Sette et al JBC 269:28 18271-18274 1994. |
| 17 | PDE1 | AC
Pathway No. 269 | Network | 2.5926 | 0.09 | No | - |
| | CaM-Dependent PDE. Amount calculated from total rate in brain vs. specific rate. |
| 18 | CaM.PDE1 | AC
Pathway No. 269 | Network | 0 | 0.09 | No | - |
| | Activity up 6x following Ca-CaM binding. |
| 19 | cAMP_in_dend | AC
Pathway No. 269 | Network | 0 | 5 | No | - |
| | Assume a 5 uM long segment of 1 uM^2 Xsection dendrite. |
| 20 | ATP | AC
Pathway No. 269 | Network | 2000 | 0.09 | Yes | - |
| | ATP is present in all cells between 2 and 10 mM. See Lehninger |
and 
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