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Molecule List for pathway PLA2 (Pathway Number 183) in Accession MAPK-bistability-fig1c (Accession Number 35)
| Default ordering is done according to Pathway Number. Table headers can be used for changing the default ordering.|
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The entries are grouped according to Pathway Number and are alternately color coded using and color.
|Buffered||Sum Total Of|
|1|| APC||Network||30||1000||Yes||- |
| || arachodonylphosphatidylcholine is the favoured substrate from Wijkander and Sundler, JBC 202 pp 873-880, 1991. Their assay used 30 uM substrate, which is what the kinetics in this model are based on. For the later model we should locate a more realistic value for APC. For now it is treated as a buffered metabolite.|
|2|| DAG-Ca-PLA2*||Network||0||1000||No||- |
|3|| PIP2-Ca-PLA2*||Network||0||1000||No||- |
|4|| PIP2-PLA2*||Network||0||1000||No||- |
|5|| PLA2*||Network||0||1000||No||- |
| || Phosphorylated PLA2. The site differs from the site phosphorylated by PKC. See Nemenoff et al 1993 JBC 268(3):1960-1964|
|6|| PLA2*-Ca||Network||0||1000||No||- |
| || Phosphorylated and active form of PLA2. Several kinases act on it: PKA: Wightman et al JBC 257 pp6650 1982 PKC: Many refs, eg Gronich et al JBC 263 pp 16645, 1988 but see Lin etal MAPK: Lin et al, Cell 72 pp 269, 1993. Show 3x with MAPK but not PKC alone The Nemenoff assays are conducted in rather high Ca so I have assumed a Ca binding step.|
|7|| PLA2-Ca*||Network||0||1000||No||- |
| || The generic Ca-activated form ofPLA2. Leslie and Channon 1990 BBA 1045:261.|
|8|| PLA2-cytosolic||Network||0.4||1000||No||- |
| || cPLA2 IV form has mol wt of 85 Kd. Glaser et al 1993 TIPS 14:92-98. Calculated cytosolic concentration is ~300 nM from Wijkander and Sundler 1991 Eur J Biochem 202:873 Leslie and Channon 1990 BBA 1045:261 use about 400 nM. Decent match. Use 400 nM. |
|Database compilation and code copyright (C) 2005, Upinder S. Bhalla and NCBS/TIFR |
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