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Enzyme Molecule /
Enzyme Activity | Accession Name / Accession No. | Pathway Name / Pathway No. | Km (uM) | kcat (s^-1) | Ratio (k2/k3) | Enzyme Type | Reagents |
1 | S6K_thr-252 / camkIII_phospho | CaMKIII
Accession No. 90 | Shared_Object_ CaMKIII Pathway No. 1093 | 1 | 1 | 4 | explicit E-S complex | Substrate: CaMKIII Product : CaMKIII*
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2 | S6K_basal / CaMKIII_basal | CaMKIII
Accession No. 90 | Shared_Object_ CaMKIII Pathway No. 1093 | 0.999896 | 10 | 4 | explicit E-S complex | Substrate: CaMKIII Product : CaMKIII*
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3 | tot_autonomous_ CaMKII / auton_305 | Ajay_Bhalla_ 2004_Feedback_ Tuning Accession No. 78 | CaMKII
Pathway No. 357 | 175.006 | 6 | 4 | explicit E-S complex | Substrate: CaMKII-thr286 Product : CaMKII***
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| See Hanson and Schulman again, for afterburst rates of phosph. |
4 | tot_CaM_CaMKII / CaM_act_305 | Ajay_Bhalla_ 2004_Feedback_ Tuning Accession No. 78 | CaMKII
Pathway No. 357 | 113.599 | 6 | 4 | explicit E-S complex | Substrate: CaMKII-thr286 Product : CaMKII***
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| Rates from autocamtide phosphorylation, from Hanson and Schulman JBC 267:24 17216-17224 1992. Jan 1 1998: Speed up 12x to match fig 5. |
5 | tot_CaM_CaMKII / CaM_act_286 | Ajay_Bhalla_ 2004_Feedback_ Tuning Accession No. 78 | CaMKII
Pathway No. 357 | 113.601 | 0.5 | 4 | explicit E-S complex | Substrate: CaMKII-CaM Product : CaMKII-thr286*-C aM
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6 | tot_autonomous_ CaMKII / auton_286 | Ajay_Bhalla_ 2004_Feedback_ Tuning Accession No. 78 | CaMKII
Pathway No. 357 | 175 | 0.5 | 4 | explicit E-S complex | Substrate: CaMKII-CaM Product : CaMKII-thr286*-C aM
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7 | PP1-active / Deph-thr286 | Ajay_Bhalla_ 2004_Feedback_ Tuning Accession No. 78 | Shared_Object_ Ajay_Bhalla_ 2004_Feedback_ Tuning Pathway No. 347 | 5.09911 | 0.35 | 4 | explicit E-S complex | Substrate: CaMKII-thr286*-C aM Product : CaMKII-CaM
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| The rates are from Stralfors et al Eur J Biochem 149 295-303 giving Vmax = 5.7 umol/min giving k3 = 3.5/sec and k2 = 14. Foulkes et al Eur J Biochem 132 309-313 1983 give Km = 5.1 uM so k1 becomes 5.72e-6 Simonelli 1984 (Grad Thesis, CUNY) showed that other substrates are about 1/10 rate of phosphorylase a, so we reduce k1,k2,k3 by 10 to 5.72e-7, 1.4, 0.35 |
8 | PP1-active / Deph-thr305 | Ajay_Bhalla_ 2004_Feedback_ Tuning Accession No. 78 | Shared_Object_ Ajay_Bhalla_ 2004_Feedback_ Tuning Pathway No. 347 | 5.09911 | 0.35 | 4 | explicit E-S complex | Substrate: CaMKII*** Product : CaMKII-thr286
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9 | PP1-active / Deph-thr286c | Ajay_Bhalla_ 2004_Feedback_ Tuning Accession No. 78 | Shared_Object_ Ajay_Bhalla_ 2004_Feedback_ Tuning Pathway No. 347 | 5.09911 | 0.35 | 4 | explicit E-S complex | Substrate: CaMKII*** Product : CaMK-thr306
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10 | PP1-active / Deph_thr286b | Ajay_Bhalla_ 2004_Feedback_ Tuning Accession No. 78 | Shared_Object_ Ajay_Bhalla_ 2004_Feedback_ Tuning Pathway No. 347 | 5.09911 | 0.35 | 4 | explicit E-S complex | Substrate: CaMKII-thr286 Product : CaMKII
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11 | tot_CaM_CaMKII / CaM_act_305 | Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. 77 | CaMKII
Pathway No. 339 | 113.599 | 6 | 4 | explicit E-S complex | Substrate: CaMKII-thr286 Product : CaMKII***
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| Rates from autocamtide phosphorylation, from Hanson and Schulman JBC 267:24 17216-17224 1992. Jan 1 1998: Speed up 12x to match fig 5. |
12 | tot_CaM_CaMKII / CaM_act_286 | Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. 77 | CaMKII
Pathway No. 339 | 113.601 | 0.5 | 4 | explicit E-S complex | Substrate: CaMKII-CaM Product : CaMKII-thr286*-C aM
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13 | tot_autonomous_ CaMKII / auton_305 | Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. 77 | CaMKII
Pathway No. 339 | 175.006 | 6 | 4 | explicit E-S complex | Substrate: CaMKII-thr286 Product : CaMKII***
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| See Hanson and Schulman again, for afterburst rates of phosph. |
14 | tot_autonomous_ CaMKII / auton_286 | Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. 77 | CaMKII
Pathway No. 339 | 175 | 0.5 | 4 | explicit E-S complex | Substrate: CaMKII-CaM Product : CaMKII-thr286*-C aM
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15 | PP1-active / Deph-thr286 | Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. 77 | Shared_Object_ Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Pathway No. 329 | 5.09911 | 0.35 | 4 | explicit E-S complex | Substrate: CaMKII-thr286*-C aM Product : CaMKII-CaM
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| The rates are from Stralfors et al Eur J Biochem 149 295-303 giving Vmax = 5.7 umol/min giving k3 = 3.5/sec and k2 = 14. Foulkes et al Eur J Biochem 132 309-313 1983 give Km = 5.1 uM so k1 becomes 5.72e-6 Simonelli 1984 (Grad Thesis, CUNY) showed that other substrates are about 1/10 rate of phosphorylase a, so we reduce k1,k2,k3 by 10 to 5.72e-7, 1.4, 0.35 |
16 | PP1-active / Deph-thr305 | Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. 77 | Shared_Object_ Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Pathway No. 329 | 5.09911 | 0.35 | 4 | explicit E-S complex | Substrate: CaMKII*** Product : CaMKII-thr286
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17 | PP1-active / Deph-thr286c | Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. 77 | Shared_Object_ Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Pathway No. 329 | 5.09911 | 0.35 | 4 | explicit E-S complex | Substrate: CaMKII*** Product : CaMK-thr306
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18 | PP1-active / Deph_thr286b | Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. 77 | Shared_Object_ Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Pathway No. 329 | 5.09911 | 0.35 | 4 | explicit E-S complex | Substrate: CaMKII-thr286 Product : CaMKII
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19 | tot_CaM_CaMKII / CaM_act_305 | Ajay_Bhalla_ 2004_PKM_Tuning Accession No. 76 | CaMKII
Pathway No. 322 | 113.599 | 6 | 4 | explicit E-S complex | Substrate: CaMKII-thr286 Product : CaMKII***
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| Rates from autocamtide phosphorylation, from Hanson and Schulman JBC 267:24 17216-17224 1992. Jan 1 1998: Speed up 12x to match fig 5. |
20 | tot_CaM_CaMKII / CaM_act_286 | Ajay_Bhalla_ 2004_PKM_Tuning Accession No. 76 | CaMKII
Pathway No. 322 | 113.601 | 0.5 | 4 | explicit E-S complex | Substrate: CaMKII-CaM Product : CaMKII-thr286*-C aM
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