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Enzyme List for CamKII acting as a Product

Entries are grouped according to Pathway Number and they are alternately color coded using

and

color.
Further ordering can be done according to header.

arrow indicates that ordering is done according to ascending or descending order.

	Enzyme Molecule / Enzyme Activity	Accession Name / Accession No.	Pathway Name / Pathway No.	Km (uM)	kcat (s^-1)	Ratio (k2/k3)	Enzyme Type	Reagents
1	S6K_thr-252 / camkIII_phospho	CaMKIII Accession No. 90	Shared_Object_ CaMKIII Pathway No. 1093	1	1	4	explicit E-S complex	Substrate: CaMKIII Product : CaMKIII*
2	S6K_basal / CaMKIII_basal	CaMKIII Accession No. 90	Shared_Object_ CaMKIII Pathway No. 1093	0.999896	10	4	explicit E-S complex	Substrate: CaMKIII Product : CaMKIII*
3	tot_autonomous_ CaMKII / auton_305	Ajay_Bhalla_ 2004_Feedback_ Tuning Accession No. 78	CaMKII Pathway No. 357	175.006	6	4	explicit E-S complex	Substrate: CaMKII-thr286 Product : CaMKII***
	See Hanson and Schulman again, for afterburst rates of phosph.
4	tot_CaM_CaMKII / CaM_act_305	Ajay_Bhalla_ 2004_Feedback_ Tuning Accession No. 78	CaMKII Pathway No. 357	113.599	6	4	explicit E-S complex	Substrate: CaMKII-thr286 Product : CaMKII***
	Rates from autocamtide phosphorylation, from Hanson and Schulman JBC 267:24 17216-17224 1992. Jan 1 1998: Speed up 12x to match fig 5.
5	tot_CaM_CaMKII / CaM_act_286	Ajay_Bhalla_ 2004_Feedback_ Tuning Accession No. 78	CaMKII Pathway No. 357	113.601	0.5	4	explicit E-S complex	Substrate: CaMKII-CaM Product : CaMKII-thr286*-C aM

6	tot_autonomous_ CaMKII / auton_286	Ajay_Bhalla_ 2004_Feedback_ Tuning Accession No. 78	CaMKII Pathway No. 357	175	0.5	4	explicit E-S complex	Substrate: CaMKII-CaM Product : CaMKII-thr286*-C aM

7	PP1-active / Deph-thr286	Ajay_Bhalla_ 2004_Feedback_ Tuning Accession No. 78	Shared_Object_ Ajay_Bhalla_ 2004_Feedback_ Tuning Pathway No. 347	5.09911	0.35	4	explicit E-S complex	Substrate: CaMKII-thr286-C aM Product :* CaMKII-CaM
	The rates are from Stralfors et al Eur J Biochem 149 295-303 giving Vmax = 5.7 umol/min giving k3 = 3.5/sec and k2 = 14. Foulkes et al Eur J Biochem 132 309-313 1983 give Km = 5.1 uM so k1 becomes 5.72e-6 Simonelli 1984 (Grad Thesis, CUNY) showed that other substrates are about 1/10 rate of phosphorylase a, so we reduce k1,k2,k3 by 10 to 5.72e-7, 1.4, 0.35
8	PP1-active / Deph-thr305	Ajay_Bhalla_ 2004_Feedback_ Tuning Accession No. 78	Shared_Object_ Ajay_Bhalla_ 2004_Feedback_ Tuning Pathway No. 347	5.09911	0.35	4	explicit E-S complex	Substrate: CaMKII* Product :** CaMKII-thr286

9	PP1-active / Deph-thr306	Ajay_Bhalla_ 2004_Feedback_ Tuning Accession No. 78	Shared_Object_ Ajay_Bhalla_ 2004_Feedback_ Tuning Pathway No. 347	5.09911	0.35	4	explicit E-S complex	Substrate: CaMK-thr306 Product : CaMKII
	See Cohen et al
10	PP1-active / Deph_thr286b	Ajay_Bhalla_ 2004_Feedback_ Tuning Accession No. 78	Shared_Object_ Ajay_Bhalla_ 2004_Feedback_ Tuning Pathway No. 347	5.09911	0.35	4	explicit E-S complex	Substrate: CaMKII-thr286 Product : CaMKII

11	tot_CaM_CaMKII / CaM_act_305	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. 77	CaMKII Pathway No. 339	113.599	6	4	explicit E-S complex	Substrate: CaMKII-thr286 Product : CaMKII***
	Rates from autocamtide phosphorylation, from Hanson and Schulman JBC 267:24 17216-17224 1992. Jan 1 1998: Speed up 12x to match fig 5.
12	tot_CaM_CaMKII / CaM_act_286	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. 77	CaMKII Pathway No. 339	113.601	0.5	4	explicit E-S complex	Substrate: CaMKII-CaM Product : CaMKII-thr286*-C aM

13	tot_autonomous_ CaMKII / auton_305	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. 77	CaMKII Pathway No. 339	175.006	6	4	explicit E-S complex	Substrate: CaMKII-thr286 Product : CaMKII***
	See Hanson and Schulman again, for afterburst rates of phosph.
14	tot_autonomous_ CaMKII / auton_286	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. 77	CaMKII Pathway No. 339	175	0.5	4	explicit E-S complex	Substrate: CaMKII-CaM Product : CaMKII-thr286*-C aM

15	PP1-active / Deph-thr286	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. 77	Shared_Object_ Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Pathway No. 329	5.09911	0.35	4	explicit E-S complex	Substrate: CaMKII-thr286-C aM Product :* CaMKII-CaM
	The rates are from Stralfors et al Eur J Biochem 149 295-303 giving Vmax = 5.7 umol/min giving k3 = 3.5/sec and k2 = 14. Foulkes et al Eur J Biochem 132 309-313 1983 give Km = 5.1 uM so k1 becomes 5.72e-6 Simonelli 1984 (Grad Thesis, CUNY) showed that other substrates are about 1/10 rate of phosphorylase a, so we reduce k1,k2,k3 by 10 to 5.72e-7, 1.4, 0.35
16	PP1-active / Deph-thr305	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. 77	Shared_Object_ Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Pathway No. 329	5.09911	0.35	4	explicit E-S complex	Substrate: CaMKII* Product :** CaMKII-thr286

17	PP1-active / Deph-thr306	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. 77	Shared_Object_ Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Pathway No. 329	5.09911	0.35	4	explicit E-S complex	Substrate: CaMK-thr306 Product : CaMKII
	See Cohen et al
18	PP1-active / Deph_thr286b	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. 77	Shared_Object_ Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Pathway No. 329	5.09911	0.35	4	explicit E-S complex	Substrate: CaMKII-thr286 Product : CaMKII

19	tot_CaM_CaMKII / CaM_act_305	Ajay_Bhalla_ 2004_PKM_Tuning Accession No. 76	CaMKII Pathway No. 322	113.599	6	4	explicit E-S complex	Substrate: CaMKII-thr286 Product : CaMKII***
	Rates from autocamtide phosphorylation, from Hanson and Schulman JBC 267:24 17216-17224 1992. Jan 1 1998: Speed up 12x to match fig 5.
20	tot_CaM_CaMKII / CaM_act_286	Ajay_Bhalla_ 2004_PKM_Tuning Accession No. 76	CaMKII Pathway No. 322	113.601	0.5	4	explicit E-S complex	Substrate: CaMKII-CaM Product : CaMKII-thr286*-C aM

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