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Molecule Parameter List for NO_sGCfull_act | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | | CondorelliGC | 19 | Pathway |
condorelliGCmod | | This model features the observations of Condorelli P, George SC. Biophys J. (2001) 80(5):2110-9. They propose a in vivo mechanism for the binding of free NO to sGC based on in vitro data within both pulmonary and vascular smooth muscle. All the parameters used have been obtained from reported results based on experiments. One important conclusion they make is that control of sGC in vivo is most likely to be ultra-sensitive and that activation in vivo occurs at lower NO concentrations than previously reported. |
NO_sGCfull_act acting as a Molecule in CondorelliGC Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | NO_sGCfull_act | CondorelliGC
Accession No. : 19 | condorelliGCmod
Pathway No. : 89 | 0 | 0.0016667 | No | | This is the fully active form of sGC, catalysing the conversion of GTP to cGMP....as proposed by Condorelli et al., 2001, Biophys J, 80:14753-14758. |
NO_sGCfull_act acting as an Enzyme in CondorelliGC Network
Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | NO_sGCfull_act /
full_act_sGC
| CondorelliGC
Accession No. : 19 | condorelliGCmod
Pathway No. : 89 | 57.9996 | 40 | 4 | explicit E-S complex | Substrate
GTP
Product
cGMP
| | Km values for the basal and fully activated forms of sGC have been determined as 85-120 uM and 58 uM, respectively ... (Ignarro et al., 1982, BBA, 718:49-59)..and the same has been used by Condorelli et al., 2001... Vmax values are around 10-100 nmol/min/mg (without NO) and 10-40 umol/min/mg (with NO) ..... thru personal correspondence with T Bellamy, UK. |
NO_sGCfull_act acting as a Substrate in a reaction in CondorelliGC Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | dissoNO | CondorelliGC
Accession No. : 19 | condorelliGCmod
Pathway No. : 89 | 0.07
(s^-1) | 0
(uM^-1 s^-1) | - | - | Substrate
NO_sGCfull_act
Product
NO
sGC_basal
| | This is a modification by Condorelli et al., 2001, Biophys J of the recent binding scheme of Zhao et al., to include the NO dissociation from fully active complex of sGC... The dissociation is characterised by the respective first order rate constants depicted in the model... Rates are obtained at 4C... |
NO_sGCfull_act acting as a Product in a reaction in CondorelliGC Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| | Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | 1 | NOdepPway | CondorelliGC
Accession No. : 19 | condorelliGCmod
Pathway No. : 89 | 1.68
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
NO_a
NO_sGCpart_act
Product
NO_sGCfull_act
| | | Rates from stopped flow experiments performed at 4C from Zhao et al., 1999, PNAS,96:14753-14758. Same rates used by Condorelli P, for his simulations... | | 2 | NOindePway | CondorelliGC
Accession No. : 19 | condorelliGCmod
Pathway No. : 89 | 0.0609
(s^-1) | 0.014
(s^-1) | Keq = 0.2299(uM) | 13.351sec | Substrate
NO_sGCpart_act
Product
NO_sGCfull_act
| | | Rates used directly from Condorelli et al., 2001, Biophysical J, 80:2110-2119 .... Rates originally from Zhao et al., 1999, PNAS, 96:14753-14758 from stopped flow spectroscopy experiments performed at 4C. |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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