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Molecule Parameter List for IP3_3K

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*IP3_3K* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	1	3	0	1	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
NonOsc_Ca_ IP3metabolism	23	Network	MIPP, CaMKII, CaM, PKC, IP3-3K, CaRegulation, Gq, PLCbeta, 134_dephos, 145_dephos, IP4-system, IHP-system, 1345_dephos
This network models detailed metabolism of Ins(145)P3, integrated with GPCR mediated PLCbeta activation and Ca release by the InsP3 receptor in the neuron. The calcium response is non-oscillatory. Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316.

IP3_3K acting as a Molecule in NonOsc_Ca_IP3metabolism Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
IP3_3K	NonOsc_Ca_ IP3metabolism Accession No. : 23	IP3-3K Pathway No. : 109	0.3853	1000	No
from Johanson et al, JBC, 1988, Vol. 263, No.16, pp 7465-7471 this is the predominant isoform in brain ie IP3-3kinaseA: BiochemJ, 1995, 306, 429-435

IP3_3K acting as an Enzyme in NonOsc_Ca_IP3metabolism Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
IP3_3K / ip3-3k	NonOsc_Ca_ IP3metabolism Accession No. : 23	IP3-3K Pathway No. : 109	1.40001	3.0475	4	explicit E-S complex	Substrate IP3(145) Product IP4(1345)
from Johanson et al, JBC 263; 1988 Original Vmax scaled up by 50% to obtain value at 37C, as enzyme assay was done at 30C Km increased from 0.2 to 1.4 as per various other reports (Shears Review, BiochemJ 260; 1989)

IP3_3K acting as a Substrate for an Enzyme in NonOsc_Ca_IP3metabolism Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	tot_CaM_CaMKII / CaM-CaMK-phos	NonOsc_Ca_ IP3metabolism Accession No. : 23	CaMKII Pathway No. : 106	1.60001	0.5	4	explicit E-S complex	Substrate IP3_3K Product IP3_3K*
	rates referred from standard CaM-CaMKII phosphorylation rates
2	tot_autonomous_ CaMKII / CaMK-phos	NonOsc_Ca_ IP3metabolism Accession No. : 23	CaMKII Pathway No. : 106	2.49999	0.5	4	explicit E-S complex	Substrate IP3_3K Product IP3_3K*
	rates referred from standard CaMKII phosphorylation rates
3	PKC-active / PKC-phos	NonOsc_Ca_ IP3metabolism Accession No. : 23	PKC Pathway No. : 108	30.0008	4	4	explicit E-S complex	Substrate IP3_3K Product IP3_3K*1
	rates referred from standard PKC phosphorylation rates

IP3_3K acting as a Substrate in a reaction in NonOsc_Ca_IP3metabolism Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
3K-bind-CaM	NonOsc_Ca_ IP3metabolism Accession No. : 23	IP3-3K Pathway No. : 109	19.2312 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 0.052(uM)	-	Substrate CaM-Ca4 IP3_3K Product IP3_3K_CaM
Communi et al, EMBO J 16; 1997 non-phosphorylated 3kinase with low sensitivity to CaM binding (Kd = 52nM)

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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