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Molecule Parameter List for PLC-Ca

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

PLC-Ca participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	1	0	0	1	2

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Osc_Ca_ IP3metabolism	24	Network	MIPP,  CaMKII,  CaM,   PKC,  IP3-3K,  Gq,   PLCbeta,  134_dephos,  145_dephos,   IP4-system,  IHP-system,  1345_dephos,   CaRegulation,  Othmer-Tang-model
This network models an oscillatory calcium response to GPCR mediated PLCbeta activation, alongwith detailed InsP3 metabolism in the neuron. It differs from the NonOsc_Ca_IP3metabolism network in the CaRegulation module and in InsP3 receptor kinetics. Details of InsP3 receptor kinetics have been adapted from the Othmer-Tang model for oscillatory Ca dynamics. Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316.

PLC-Ca acting as a Molecule in  Osc_Ca_IP3metabolism Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
PLC-Ca	Osc_Ca_ IP3metabolism Accession No. : 24	PLCbeta Pathway No. : 126	0	1000	No
From Sternweis et al Phil Trans R Soc Lond 1992, also matched by Homma et al. k1 = 1.5e-5, now 4.2e-6 k2 = 70/sec; now 40/sec k3 = 17.5/sec; now 10/sec Note that the wording in Sternweis et al is ambiguous re the Km. Also Smrcka et al; Science 251, 15.2.1991, pp804-807

PLC-Ca acting as an Enzyme in  Osc_Ca_IP3metabolism Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
PLC-Ca / PLC-Ca	Osc_Ca_ IP3metabolism Accession No. : 24	PLCbeta Pathway No. : 126	19.8408	10	4	explicit E-S complex	Substrate PIP2 Product DAG IP3(145)
From Sternweis et al Phil Trans R Soc Lond 1992, also matched by Homma et al. k1 = 1.5e-5, now 4.2e-6 k2 = 70/sec; now 40/sec k3 = 17.5/sec; now 10/sec Note that the wording in Sternweis et al is ambiguous re the Km. Also Smrcka et al; Science 251, 15.2.1991, pp804-807

PLC-Ca acting as a Substrate in a reaction in  Osc_Ca_IP3metabolism Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
Act-PLC-by-Gq	Osc_Ca_ IP3metabolism Accession No. : 24	PLCbeta Pathway No. : 126	25.2 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 0.0397(uM)	-	Substrate G*GTP PLC-Ca Product PLC-Ca-Gq
Affinity for Gq is > 20 nM (Smrcka et al Science251 804-807 1991) so [Gq].kf = kb so 40nM * 6e5 = kb/kf = 24e3 so kf = 4.2e-5, kb =1

PLC-Ca acting as a Product in a reaction in  Osc_Ca_IP3metabolism Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	Act-PLC-Ca	Osc_Ca_ IP3metabolism Accession No. : 24	PLCbeta Pathway No. : 126	3 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 0.3333(uM)	-	Substrate Ca PLC Product PLC-Ca
	Affinity for Ca = 1uM without AlF, 0.1 with: from Smrcka et al science 251 pp 804-807 1991 so [Ca].kf = kb so kb/kf = 1 * 6e5 = 1/1.66e-6 Assigned affinity to a Kd of 0.333 to maintain balance.
2	Inact-PLC-Gq	Osc_Ca_ IP3metabolism Accession No. : 24	PLCbeta Pathway No. : 126	1.6667 (s^-1)	0 (uM^-1 s^-1)	-	-	Substrate PLC-Ca-Gq Product G*GDP PLC-Ca
	Rate of 100/min to account for GAP activity of PLC: Biddlecome et al, JBC, 271, 14, 7999-8007, 1996