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Molecule Parameter List for PIP2

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

PIP2 participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	4	0	1	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
NonOsc_Ca_ IP3metabolism	31	Network	MIPP,  CaMKII,  CaM,   PKC,  IP3-3K,  CaRegulation,   Gq,  PLCbeta,  134_dephos,   145_dephos,  IP4-system,  IHP-system,   1345_dephos
This network models detailed metabolism of Ins(145)P3, integrated with GPCR mediated PLCbeta activation and Ca release by the InsP3 receptor in the neuron. It is similar to the NonOsc_Ca_IP3metab model (accession 23) except that some enzymes have been modified to have reversible kinetics rather than Michaelis-Menten kinetics. These modified enzymes belong to the groups: IP4-system, IP3-3K, 145_dephos and 134_dephos. Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316.

PIP2 acting as a Molecule in  NonOsc_Ca_IP3metabolism Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
PIP2	NonOsc_Ca_ IP3metabolism Accession No. : 31	PLCbeta Pathway No. : 151	150	1000	Yes
PIP2 conc: Willars et al; JBC 273 (9) 27.2.98; pp 5037-5046

PIP2 acting as a Substrate for an Enzyme in  NonOsc_Ca_IP3metabolism Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	PLC-Gq  / PLC-Gq	NonOsc_Ca_ IP3metabolism Accession No. : 31	PLCbeta Pathway No. : 151	5	75	4	explicit E-S complex	Substrate PIP2 Product DAG IP3(145)
	from Smrcka et al, 1991 Science 251: 804-807
2	PLC-Ca  / PLC-Ca	NonOsc_Ca_ IP3metabolism Accession No. : 31	PLCbeta Pathway No. : 151	19.8408	10	4	explicit E-S complex	Substrate PIP2 Product DAG IP3(145)
	From Sternweis et al Phil Trans R Soc Lond 1992, also matched by Homma et al. k1 = 1.5e-5, now 4.2e-6 k2 = 70/sec; now 40/sec k3 = 17.5/sec; now 10/sec Note that the wording in Sternweis et al is ambiguous re the Km. Also Smrcka et al; Science 251, 15.2.1991, pp804-807
3	PLC-Ca-Gq  / PLCb-Ca-Gq	NonOsc_Ca_ IP3metabolism Accession No. : 31	PLCbeta Pathway No. : 151	4.99994	160	4	explicit E-S complex	Substrate PIP2 Product DAG IP3(145)
	Km: Sternweis et al, Phil Trans R Soc Lond 1992 Vmax: Smrcka et al, Science 1991
4	PLC  / PLC	NonOsc_Ca_ IP3metabolism Accession No. : 31	PLCbeta Pathway No. : 151	19.9994	2.5	4	explicit E-S complex	Substrate PIP2 Product DAG IP3(145)
	Smrcka et al; Science 251, 15.2.1991, pp804-807

PIP2 acting as a Substrate in a reaction in  NonOsc_Ca_IP3metabolism Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
basal	NonOsc_Ca_ IP3metabolism Accession No. : 31	PLCbeta Pathway No. : 151	0.003 (s^-1)	0 (uM^-1 s^-1)	-	-	Substrate PIP2 Product DAG IP3(145)
accounts for other PLC isoforms that contribute to basal levels of IP3