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Molecule Parameter List for DAG | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | Osc_Ca_
IP3metabolism | 32 | Network |
MIPP, CaMKII, CaM,
PKC, IP3-3K, Gq,
PLCbeta, 134_dephos, 145_dephos,
IP4-system, IHP-system, 1345_dephos,
CaRegulation, Othmer-Tang-model | | This network models an oscillatory calcium response to GPCR mediated PLCbeta activation, alongwith detailed InsP3 metabolism in the neuron. It is similar to the Osc_Ca_IP3metab model (accession 24) except that some enzymes in the InsP3 metabolism network have been modified to have reversible kinetics rather than Michaelis-Menten kinetics. The modified enzymes belong to the groups: IP4-system, IP3-3K, 145_dephos and 134_dephos. Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316. |
DAG acting as a Molecule in Osc_Ca_IP3metabolism Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | DAG | Osc_Ca_
IP3metabolism
Accession No. : 32 | PLCbeta
Pathway No. : 164 | 0 | 1000 | No | | Basal levels of Diacylglycerol in model are 5.06 uM. DAG is pretty nasty to estimate. Data sources are many and varied and sometimes difficult to reconcile. Welsh and Cabot 1987 JCB 35:231-245: DAG degradation Bocckino et al JBC 260(26):14201-14207: hepatocytes stim with vasopressin: 190 uM. Bocckino et al 1987 JBC 262(31):15309-15315: DAG rises from 70 to 200 ng/mg wet weight, approx 150 to 450 uM. Prescott and Majerus 1983 JBC 258:764-769: Platelets: 6 uM. Also see Rittenhouse-Simmons 1979 J Clin Invest 63. Sano et al JBC 258(3):2010-2013: Report a nearly 10 fold rise. Habenicht et al 1981 JBC 256(23)12329-12335: 3T3 cells with PDGF stim: 27 uM Cornell and Vance 1987 BBA 919:23-36: 10x rise from 10 to 100 uM |
DAG acting as a Product of an Enzyme in Osc_Ca_IP3metabolism Network
| | Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | | 1 | PLC-Gq /
PLC-Gq | Osc_Ca_
IP3metabolism
Accession No. : 32 | PLCbeta
Pathway No. : 164 | 5 | 75 | 4 | explicit E-S complex | Substrate
PIP2
Product
DAG
IP3(145)
| | | from Smrcka et al, 1991 Science 251: 804-807 | | 2 | PLC-Ca /
PLC-Ca | Osc_Ca_
IP3metabolism
Accession No. : 32 | PLCbeta
Pathway No. : 164 | 19.8408 | 10 | 4 | explicit E-S complex | Substrate
PIP2
Product
DAG
IP3(145)
| | | From Sternweis et al Phil Trans R Soc Lond 1992, also matched by Homma et al. k1 = 1.5e-5, now 4.2e-6 k2 = 70/sec; now 40/sec k3 = 17.5/sec; now 10/sec Note that the wording in Sternweis et al is ambiguous re the Km. Also Smrcka et al; Science 251, 15.2.1991, pp804-807 | | 3 | PLC-Ca-Gq /
PLCb-Ca-Gq | Osc_Ca_
IP3metabolism
Accession No. : 32 | PLCbeta
Pathway No. : 164 | 4.99994 | 160 | 4 | explicit E-S complex | Substrate
PIP2
Product
DAG
IP3(145)
| | | Km: Sternweis et al, Phil Trans R Soc Lond 1992 Vmax: Smrcka et al, Science 1991 | | 4 | PLC /
PLC | Osc_Ca_
IP3metabolism
Accession No. : 32 | PLCbeta
Pathway No. : 164 | 19.9994 | 2.5 | 4 | explicit E-S complex | Substrate
PIP2
Product
DAG
IP3(145)
| | | Smrcka et al; Science 251, 15.2.1991, pp804-807 |
DAG acting as a Substrate in a reaction in Osc_Ca_IP3metabolism Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| | Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | 1 | PKC-act-by-DAG | Osc_Ca_
IP3metabolism
Accession No. : 32 | PKC
Pathway No. : 161 | 0.008
(uM^-1 s^-1) | 8.6348
(s^-1) | Kd(bf) = 1079.377(uM) | - | Substrate
DAG
PKC-Ca
Product
PKC-Ca-DAG
| | | Ca.PKC interaction with DAG is modeled by this reaction. Kf based on Shinomura et al PNAS 88 5149-5153 1991 and Schaechter and Benowitz 1993 J Neurosci 13(10):4361 and uses the constraining procedure referred to in the general notes for PKC. | | 2 | PKC-n-DAG | Osc_Ca_
IP3metabolism
Accession No. : 32 | PKC
Pathway No. : 161 | 0.0006
(uM^-1 s^-1) | 0.1
(s^-1) | Kd(bf) = 166.6667(uM) | - | Substrate
DAG
PKC-cytosolic
Product
PKC-DAG
| | | Binding of PKC to DAG, non-Ca dependent. Kf based on Shinomura et al PNAS 88 5149-5153 1991 Tau estimated as fast and here it is about the same time-course as the formation of DAG so it will not be rate-limiting. | | 3 | Degrade-DAG | Osc_Ca_
IP3metabolism
Accession No. : 32 | PLCbeta
Pathway No. : 164 | 0.15
(s^-1) | 0
(s^-1) | - | - | Substrate
DAG
Product
PC
| | | Rate based on basal and activated levels of DAG |
DAG acting as a Product in a reaction in Osc_Ca_IP3metabolism Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | basal | Osc_Ca_
IP3metabolism
Accession No. : 32 | PLCbeta
Pathway No. : 164 | 0.003
(s^-1) | 0
(uM^-1 s^-1) | - | - | Substrate
PIP2
Product
DAG
IP3(145)
| | accounts for other PLC isoforms that contribute to basal levels of IP3 |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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