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Molecule Parameter List for EGF

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*EGF* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	0	1	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
EGFR_Signaling_ pathway	52	Pathway	EGFR
This model is based on the model and parameters published in paper: Quantification of Short Term Signaling by the EGFR Kholodenko et al, The J Biol Chem. , Vol 274, No. 42, Issue of October 15, pp. 30169 - 30181, 1999. Note: There are a few small ambiguities in the paper about initial conditions employed for obtaining the results. Thanks to Dr. Kholodenko for clarifying those ambiguities and providing the values used in the simulations:[G-S]=33.04579, [Grb]=51.95431, [SOS]=0.95421.

EGF acting as a Molecule in EGFR_Signaling_pathway Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
EGF	EGFR_Signaling_ pathway Accession No. : 52	EGFR Pathway No. : 223	0.666	1.6667E-6	No
Taken from Kholodenko et al 1999 Simulations were done at EGF concentration set to 20nM and 2nM concentration. This concentration was multiplied by a constant factor Vm/Vcw = 33.3 to formally rescale it to the cytoplasmic water volume. So actual concentration to which EGF was set was 666nM and 66.6nM. Plots 1&3 of figure-5 and plot 1 of figure 4 are obtained by setting the EGF conc to 666nM. Plots 2&4 of figure-5 and plot 3 of figure 4 are obtained by setting the EGF conc to 66.6nM.

EGF acting as a Substrate in a reaction in EGFR_Signaling_pathway Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
r1	EGFR_Signaling_ pathway Accession No. : 52	EGFR Pathway No. : 223	3 (uM^-1 s^-1)	0.06 (s^-1)	Kd(bf) = 0.02(uM)	-	Substrate EGF R Product Ra
Taken from Kholodenko et al 1999.

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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