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Molecule Parameter List for Sh-G

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

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Statistics

*Sh-G* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	2	0	0	0	1	2

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
EGFR_Signaling_ pathway	52	Pathway	EGFR
This model is based on the model and parameters published in paper: Quantification of Short Term Signaling by the EGFR Kholodenko et al, The J Biol Chem. , Vol 274, No. 42, Issue of October 15, pp. 30169 - 30181, 1999. Note: There are a few small ambiguities in the paper about initial conditions employed for obtaining the results. Thanks to Dr. Kholodenko for clarifying those ambiguities and providing the values used in the simulations:[G-S]=33.04579, [Grb]=51.95431, [SOS]=0.95421.

Sh-G acting as a Molecule in EGFR_Signaling_pathway Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
Sh-G	EGFR_Signaling_ pathway Accession No. : 52	EGFR Pathway No. : 223	0	1.6667E-6	No
Taken from Kholodenko BN et al J Biol Chem. (1999) 274(42):30169-81.

Sh-G acting as a Summed Molecule in EGFR_Signaling_pathway Network

	Accession Nane	Pathway Name	Target	Input
1	EGFR_Signaling_ pathway Accession No. : 52	EGFR Pathway No. : 223	pShctot	R-ShP R-Sh-G R-Sh-G-S ShP Sh-G Sh-G-S
	Taken from Kholodenko BN et al J Biol Chem. (1999) 274(42):30169-81. Total phosphorylated Shc = [ShP] + [Sh-G] + [ Sh-G-S] + [R-ShP] [R-Sh-G] + [R-Sh-G-S]
2	EGFR_Signaling_ pathway Accession No. : 52	EGFR Pathway No. : 223	Grb2_Shc	R-Sh-G Sh-G R-Sh-G-S Sh-G-S
	Taken from Kholodenko BN et al J Biol Chem. (1999) 274(42):30169-81. Total concentration of Grb2 bound to Shc isoforms= [Sh-G-S] [R-Sh-G] +[R-Sh-G-S] + [Sh-G]

Sh-G acting as a Substrate in a reaction in EGFR_Signaling_pathway Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
r22	EGFR_Signaling_ pathway Accession No. : 52	EGFR Pathway No. : 223	30 (uM^-1 s^-1)	0.064 (s^-1)	Kd(bf) = 0.0021(uM)	-	Substrate SOS Sh-G Product Sh-G-S
Taken from Kholodenko BN et al J Biol Chem. (1999) 274(42):30169-81.

Sh-G acting as a Product in a reaction in EGFR_Signaling_pathway Network

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	r18	EGFR_Signaling_ pathway Accession No. : 52	EGFR Pathway No. : 223	0.3 (s^-1)	0.9 (uM^-1 s^-1)	Kd(cb) = 3(uM)	-	Substrate R-Sh-G Product RP Sh-G
	Taken from Kholodenko BN et al J Biol Chem. (1999) 274(42):30169-81.
2	r21	EGFR_Signaling_ pathway Accession No. : 52	EGFR Pathway No. : 223	3 (uM^-1 s^-1)	0.1 (s^-1)	Kd(bf) = 0.0333(uM)	-	Substrate Grb ShP Product Sh-G
	Taken from Kholodenko BN et al J Biol Chem. (1999) 274(42):30169-81.

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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