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Molecule Parameter List for PPhosphatase2A

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*PPhosphatase2A* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	4	0	0	0	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
EGFR_MAPK	58	Network	Shared_Object_EGFR_MAPK, MAPK, Ras, EGFR, Sos
Model of MAPK activation by EGFR in the synapse. Demonstration programs using this model are available here. Primary citation:Bhalla US. Biophys J. (2004) 87(2):745-53.

PPhosphatase2A acting as a Molecule in EGFR_MAPK Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
PPhosphatase2A	EGFR_MAPK Accession No. : 58	Shared_Object_ EGFR_MAPK Pathway No. : 229	0.224	1000	No
Refs: Pato et al Biochem J 293:35-41(93); Takai&Mieskes Biochem J 275:233-239 k1=1.46e-4, k2=1000,k3=250. these use kcat values for calponin. Also, units of kcat may be in min! revert to Vmax base: k3=6, k2=25,k1=3.3e-6 or 6,6,1e-6 CoInit assumed 0.1 uM. See NOTES for MAPK_Ras50.g. CoInit now 0.08 Sep 17 1997: Raise CoInt 1.4x to 0.224, see parm searches from jun 96 on.

PPhosphatase2A acting as an Enzyme in EGFR_MAPK Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	PPhosphatase2A / craf-deph	EGFR_MAPK Accession No. : 58	Shared_Object_ EGFR_MAPK Pathway No. : 229	15.6566	6	4.16667	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
2	PPhosphatase2A / MAPKK-deph	EGFR_MAPK Accession No. : 58	Shared_Object_ EGFR_MAPK Pathway No. : 229	15.6566	6	4.16667	explicit E-S complex	Substrate MAPKK* Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.
3	PPhosphatase2A / MAPKK-deph-ser	EGFR_MAPK Accession No. : 58	Shared_Object_ EGFR_MAPK Pathway No. : 229	15.6566	6	4.16667	explicit E-S complex	Substrate MAPKK-ser Product MAPKK
4	PPhosphatase2A / craf**-deph	EGFR_MAPK Accession No. : 58	Shared_Object_ EGFR_MAPK Pathway No. : 229	15.6566	6	4.16667	explicit E-S complex	Substrate craf-1 Product** craf-1*
	Ueki et al JBC 269(22) pp 15756-15761 1994 show hyperphosphorylation of craf, so this is there to dephosphorylate it. Identity of phosphatase is not known to me, but it may be PP2A like the rest, so I have made it so.

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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