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Molecule Parameter List for Lambda-cl_operator

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

Lambda-cl_operator participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	1	0	0	1	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Quorum_ repressilator	69	Network	Shared_Object_Quorum_repressilator,  TetR,  LacI,   Lambda-cl,  LacI_2nd_copy,  LuxI
This model represents a single cell with the repressilator circuit, which is part of a population of similar cells. The repressilator circuit is a network of three proteins, i.e. LacI, TetR, Lambda-cI. The single repressilator model is at accession number 68 in the DOQCS database. In the current model, the LacI is coupled with LuxI which forms an Autoinducer(AI) protein, which couples to LuxR which activates LacI. The AI protein difffuses across the cell membrane so that the population of cells can interact. The lifetime ratio is the ratio between the mRNA and protein lifetimes. This ratio in different cells is chosen from a random gaussian distribution of mean 1 and standard deviation 0.05. The lifetime ratio of this example cell is 0.9837

Lambda-cl_operator acting as a Molecule in  Quorum_repressilator Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
Lambda-cl_operator	Quorum_ repressilator Accession No. : 69	Lambda-cl Pathway No. : 301	0.0222	3	No
The lambda cl operator under normal conditions drives transcription of lambda cl Elowitz, M. et al. (2000) Nature 403:335-338

Lambda-cl_operator acting as an Enzyme in  Quorum_repressilator Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
Lambda-cl_ operator / Lambda-cl_ basal_ transcription	Quorum_ repressilator Accession No. : 69	Lambda-cl Pathway No. : 301	0.000555607	216	4	Classical Michaelis-Menten V = Etot.S.Kcat/Km+S	Substrate Nucleotides Product lambda-cl_mRNA1
Basal transcription of the Lambda cl gene. Rates from Fig 2 Garcia and Elowitz et al. (2004) PNAS 101:10955-60

Lambda-cl_operator acting as a Substrate in a reaction in  Quorum_repressilator Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
Lambda-cl_ operator_ inhibition_ reaction	Quorum_ repressilator Accession No. : 69	Lambda-cl Pathway No. : 301	1000.01 (uM^-1 s^-1)	224 (s^-1)	Kd(bf) = 0.224(uM)	-	Substrate Lambda-cl_ operator TetR-1 Product Lambda-cl_ operator_1st_ bound
It signifies the first binding of the repressor with the operator Kf = 1 /nM/sec Kb = 224 /sec Box 1 Elowitz, M. et al. (2000) Nature 403:335-338