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Molecule Parameter List for Lambda-cl_operator | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | Quorum_
repressilator | 69 | Network |
Shared_Object_Quorum_repressilator, TetR, LacI,
Lambda-cl, LacI_2nd_copy, LuxI | This model represents a single cell with the repressilator circuit, which is part of a population of similar cells. The repressilator circuit is a network of three proteins, i.e. LacI, TetR, Lambda-cI.
The single repressilator model is at accession number 68 in the DOQCS database.
In the current model, the LacI is coupled with LuxI which forms an Autoinducer(AI) protein, which couples to LuxR which activates LacI. The AI protein difffuses across the cell membrane so that the population of cells can interact. The lifetime ratio is the ratio between the mRNA and protein lifetimes. This ratio in different cells is chosen from a random gaussian distribution of mean 1 and standard deviation 0.05. The lifetime ratio of this example cell is 0.9837 |
Lambda-cl_operator acting as a Molecule in Quorum_repressilator Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | Lambda-cl_operator | Quorum_
repressilator
Accession No. : 69 | Lambda-cl
Pathway No. : 301 | 0.0222 | 3 | No | | The lambda cl operator under normal conditions drives transcription of lambda cl Elowitz, M. et al. (2000) Nature 403:335-338 |
Lambda-cl_operator acting as an Enzyme in Quorum_repressilator Network
Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | Lambda-cl_
operator /
Lambda-cl_
basal_
transcription
| Quorum_
repressilator
Accession No. : 69 | Lambda-cl
Pathway No. : 301 | 0.000555607 | 216 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | Substrate
Nucleotides
Product
lambda-cl_mRNA1
| | Basal transcription of the Lambda cl gene. Rates from Fig 2 Garcia and Elowitz et al. (2004) PNAS 101:10955-60 |
Lambda-cl_operator acting as a Substrate in a reaction in Quorum_repressilator Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | Lambda-cl_
operator_
inhibition_
reaction | Quorum_
repressilator
Accession No. : 69 | Lambda-cl
Pathway No. : 301 | 1000.01
(uM^-1 s^-1) | 224
(s^-1) | Kd(bf) = 0.224(uM) | - | Substrate
Lambda-cl_
operator
TetR-1
Product
Lambda-cl_
operator_1st_
bound
| | It signifies the first binding of the repressor with the operator Kf = 1 /nM/sec Kb = 224 /sec Box 1 Elowitz, M. et al. (2000) Nature 403:335-338 |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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