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Molecule Parameter List for PIP2-Ca-PLA2*

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

PIP2-Ca-PLA2* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	1	0	0	0	1

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Ajay_Bhalla_ 2004_Feedback_ Tuning	78	Network	Shared_Object_Ajay_Bhalla_2004_Feedback_Tuning, PKC, PLA2, PLCbeta, Gq, MAPK, Ras, EGFR, Sos, PLC_g, CaMKII, CaM, PP1, PP2B, PKA, AC
This model is taken from Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80. This is the feedback model from Figure 8a.

PIP2-Ca-PLA2* acting as a Molecule in Ajay_Bhalla_2004_Feedback_Tuning Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
PIP2-Ca-PLA2*	Ajay_Bhalla_ 2004_Feedback_ Tuning Accession No. : 78	PLA2 Pathway No. : 349	0	1.5	No

PIP2-Ca-PLA2* acting as an Enzyme in Ajay_Bhalla_2004_Feedback_Tuning Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
PIP2-Ca-PLA2* / kenz	Ajay_Bhalla_ 2004_Feedback_ Tuning Accession No. : 78	PLA2 Pathway No. : 349	20	36	4	explicit E-S complex	Substrate APC Product AA
10 x raise oct 22 12 x and rescale for k2 = 4 * k3 convention oct 24 Increase further to get the match to expt, which was spoilt due to large accumulation of PLA2 in the enzyme complexed forms. Lets raise k3, leaving the others at k1 = 1.5e-5 and k2 = 144 since they are large already.

PIP2-Ca-PLA2* acting as a Product in a reaction in Ajay_Bhalla_2004_Feedback_Tuning Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
PIP2-Ca-PLA2-act	Ajay_Bhalla_ 2004_Feedback_ Tuning Accession No. : 78	PLA2 Pathway No. : 349	0.012 (uM^-1 s^-1)	0.1 (s^-1)	Kd(bf) = 8.3335(uM)	-	Substrate PLA2-Ca* temp-PIP2 Product PIP2-Ca-PLA2*

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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