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Molecule Parameter List for MAPK*

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

MAPK* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	2	1	1	0	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Ajay_Bhalla_ 2007_Bistable	79	Network	Shared_Object_Ajay_Bhalla_2007_Bistable,  PKC,  PLA2,   MAPK,  Ras,  CaM
This is a model of ERKII signaling which is bistable due to feedback. The feedback occurs through ERKII phosphorylation of phospholipase A2 (PLA2), leading to increased production of arachidonic acid (AA), which activates protein kinase C (PKC) which activates c-Raf which is upstream of ERKII. The model is a highly simplified variant of more detailed bistable models of MAPK signaling (Bhalla US, Iyengar R. Science. 1999 Jan 15;283(5400):381-7, Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80)

MAPK* acting as a Molecule in  Ajay_Bhalla_2007_Bistable Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
MAPK*	Ajay_Bhalla_ 2007_Bistable Accession No. : 79	Shared_Object_ Ajay_Bhalla_ 2007_Bistable Pathway No. : 363	0	125.7	No

MAPK* acting as an Enzyme in  Ajay_Bhalla_2007_Bistable Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	MAPK* / MAPK*	Ajay_Bhalla_ 2007_Bistable Accession No. : 79	Shared_Object_ Ajay_Bhalla_ 2007_Bistable Pathway No. : 363	25.6402	10	4	explicit E-S complex	Substrate PLA2-cytosolic Product PLA2*
	Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5
2	MAPK* / MAPK*-feedback	Ajay_Bhalla_ 2007_Bistable Accession No. : 79	Shared_Object_ Ajay_Bhalla_ 2007_Bistable Pathway No. : 363	25.6402	10	4	explicit E-S complex	Substrate craf-1* Product craf-1**
	Ueki et al JBC 269(22):15756-15761 show the presence of this step, but not the rate consts, which are derived from Sanghera et al JBC 265(1):52-57, 1990, see the deriv in the MAPK* notes.

MAPK* acting as a Substrate for an Enzyme in  Ajay_Bhalla_2007_Bistable Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
MKP-1  / MKP1-thr-deph	Ajay_Bhalla_ 2007_Bistable Accession No. : 79	Shared_Object_ Ajay_Bhalla_ 2007_Bistable Pathway No. : 363	0.133331	4	4	explicit E-S complex	Substrate MAPK* Product MAPK-tyr
See MKP1-tyr-deph

MAPK* acting as a Product of an Enzyme in  Ajay_Bhalla_2007_Bistable Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
MAPKK*  / MAPKKthr	Ajay_Bhalla_ 2007_Bistable Accession No. : 79	MAPK Pathway No. : 366	0.0462968	0.3	4	explicit E-S complex	Substrate MAPK-tyr Product MAPK*
Rate consts same as for MAPKKtyr.