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Molecule Parameter List for MKP-1

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
MKP-1 participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1020000

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
3d_fold_model8Network
Shared_Object_3d_fold_model,  PKC,  MAPK,  
PLA2,  Ras 
This model is an annotated version of the synaptic signaling network.
The primary reference is Bhalla US and Iyengar R. Science (1999) 283(5400):381-7 but several of the model pathways have been updated Bhalla US, Ram PT, Iyengar R. Science. 2002 Aug 9;297(5583):1018-23.

MKP-1 acting as a Molecule in  3d_fold_model Network
NameAccession NamePathway NameInitial Conc.
(uM)		Volume
(fL)		Buffered
MKP-13d_fold_model
Accession No. : 8
  • Shared_Object_
    3d_fold_model
    Pathway No. : 54
    		• 0.00321000No
    MKP-1 dephosphoryates and inactivates MAPK in vivo: Sun et al Cell 75 487-493 1993. Levels of MKP-1 are regulated, and rise in 1 hour. Kinetics from Charles et al PNAS 90:5292-5296 1993. They refer to Charles et al Oncogene 7 187-190 who show that half-life of MKP1/3CH134 is 40 min. 80% deph of MAPK in 20 min Sep 17 1997: CoInit now 0.4x to 0.0032. See parm searches from jun96 on.

    MKP-1 acting as an Enzyme in      3d_fold_model Network
     Enzyme Molecule /
    Enzyme Activity    		Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    1MKP-1 /
    MKP1-tyr-deph
    		3d_fold_model
    Accession No. : 8
  • Shared_Object_
    3d_fold_model
    Pathway No. : 54
    		• 0.066666714explicit E-S complexSubstrate
    MAPK-tyr

    Product
    MAPK
        The original kinetics have been modified to obey the k2 = 4 * k3 rule, while keeping kcat and Km fixed. As noted in the NOTES, the only constraining data point is the time course of MAPK dephosphorylation, which this model satisfies. It would be nice to have more accurate estimates of rate consts and MKP-1 levels from the literature. Effective Km : 67 nM kcat = 1.43 umol/min/mg
    2MKP-1 /
    MKP1-thr-deph
    		3d_fold_model
    Accession No. : 8
  • Shared_Object_
    3d_fold_model
    Pathway No. : 54
    		• 0.066666714explicit E-S complexSubstrate
    MAPK*

    Product
    MAPK-tyr
        See MKP1-tyr-deph


    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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