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Molecule Parameter List for craf-1

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

craf-1 participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	25	0	0	25	25	25	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Ajay_bhalla_ 2007_ReacDiff1_ 1e-12	81	Network	Shared_Object_Ajay_bhalla_2007_ReacDiff1_1e-12,   PKC,  MAPK,  Ras,  CaM,  PKM,  chain,  kinetics,  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[1],   PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[2],  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[3],   PKC,  MAPK,  MAPK,  Ras,  CaM,  PKM,  kinetics[4],  PKC,  MAPK,  Ras,  CaM,  PKM,   kinetics[5],  PKC,  MAPK,  Ras,  kinetics[6],  CaM,  PKM,  PKC,  MAPK,  Ras,   CaM,  PKM,  kinetics[7],  PKC,  Ras,  CaM,  PKM,  kinetics[8],  PKC,  MAPK,   Ras,  CaM,  PKM,  kinetics[9],  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[10],   PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[11],  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[12],   PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[13],  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[14],   PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[15],  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[16],   PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[17],  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[18],   PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[19],  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[20],   PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[21],  PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[22],   PKC,  MAPK,  Ras,  CaM,  PKM,  kinetics[23],  PKC,  MAPK,  Ras,  CaM,  PKM
This is a 25-compartment reaction-diffusion version of the Ajay_Bhalla_2007_PKM model. The original single-compartment model is repeated 25 times. In addition, a subset (27 out of 42) molecules can diffuse between compartments. Diffusion is implemented as a reaction between corresponding molecules in neighboring compartments. For D = 1e-12 m^2/sec (i.e., 1 micron^2/sec ) the kf and kb of this reaction for these 10 micron compartments are both 0.01/sec. For D = 1e-13 m^2/sec (i.e., 0.1 micron^2/sec ) the kf and kb are 0.001/sec. The stimulus file pkm_mapk22_diff_1e-12_Fig4A which was used for the model to replicate Figure 4A from the paper. This stimulus file pkm_mapk22_diff_1e-12_Fig4G which was used for the model to replicate Figure 4G from the paper

craf-1 acting as a Molecule in  Ajay_bhalla_2007_ReacDiff1_1e-12 Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
craf-1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 377	0.5	1.5	No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
craf-1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 384	0.5	1.5	No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
craf-1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 390	0.5	1.5	No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
craf-1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 396	0.5	1.5	No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
craf-1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 403	0.5	1.5	No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
craf-1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 409	0.5	1.5	No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
craf-1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 415	0.5	1.5	No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
craf-1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 421	0.5	1.5	No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
craf-1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 402	0.5	1.5	No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
craf-1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 432	0.5	1.5	No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
craf-1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 438	0.5	1.5	No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
craf-1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 444	0.5	1.5	No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
craf-1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 450	0.5	1.5	No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
craf-1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 456	0.5	1.5	No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
craf-1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 462	0.5	1.5	No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
craf-1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 468	0.5	1.5	No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
craf-1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 474	0.5	1.5	No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
craf-1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 480	0.5	1.5	No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
craf-1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 486	0.5	1.5	No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
craf-1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 492	0.5	1.5	No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
craf-1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 498	0.5	1.5	No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
craf-1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 504	0.5	1.5	No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
craf-1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 510	0.5	1.5	No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
craf-1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 516	0.5	1.5	No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006
craf-1	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	MAPK Pathway No. : 522	0.5	1.5	No
Couldn't find any ref to the actual conc of craf-1 but I should try Strom et al Oncogene 5 pp 345 In line with the other kinases in the cascade, I estimate the conc to be 0.2 uM. To init we use 0.15, which is close to equil 16 May 2003: Changing to synaptic levels. Increasing 2.5 fold to 0.5 uM. See Mihaly et al 1991 Brain Res 547(2):309-14 and Morice et al 1999 Eur J Neurosci 11(6):1995-2006

craf-1 acting as a Substrate for an Enzyme in  Ajay_bhalla_2007_ReacDiff1_1e-12 Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	PKC-active  / PKC-act-raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	Shared_Object_ Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Pathway No. : 375	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
2	PKC-active  / PKC-act-raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics Pathway No. : 382	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
3	PKC-active  / PKC-act-raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[1] Pathway No. : 388	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
4	PKC-active  / PKC-act-raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[2] Pathway No. : 394	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
5	PKC-active  / PKC-act-raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[3] Pathway No. : 400	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
6	PKC-active  / PKC-act-raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[4] Pathway No. : 407	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
7	PKC-active  / PKC-act-raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[5] Pathway No. : 413	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
8	PKC-active  / PKC-act-raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[6] Pathway No. : 417	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
9	PKC-active  / PKC-act-raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[7] Pathway No. : 425	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
10	PKC-active  / PKC-act-raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[8] Pathway No. : 430	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
11	PKC-active  / PKC-act-raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[9] Pathway No. : 436	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
12	PKC-active  / PKC-act-raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[10] Pathway No. : 442	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
13	PKC-active  / PKC-act-raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[11] Pathway No. : 448	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
14	PKC-active  / PKC-act-raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[12] Pathway No. : 454	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
15	PKC-active  / PKC-act-raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[13] Pathway No. : 460	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
16	PKC-active  / PKC-act-raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[14] Pathway No. : 466	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
17	PKC-active  / PKC-act-raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[15] Pathway No. : 472	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
18	PKC-active  / PKC-act-raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[16] Pathway No. : 478	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
19	PKC-active  / PKC-act-raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[17] Pathway No. : 484	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
20	PKC-active  / PKC-act-raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[18] Pathway No. : 490	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
21	PKC-active  / PKC-act-raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[19] Pathway No. : 496	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
22	PKC-active  / PKC-act-raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[20] Pathway No. : 502	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
23	PKC-active  / PKC-act-raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[21] Pathway No. : 508	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
24	PKC-active  / PKC-act-raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[22] Pathway No. : 514	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
25	PKC-active  / PKC-act-raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[23] Pathway No. : 520	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC

craf-1 acting as a Product of an Enzyme in  Ajay_bhalla_2007_ReacDiff1_1e-12 Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	PPhosphatase2A  / craf-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	Shared_Object_ Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Pathway No. : 375	15.6567	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
2	PPhosphatase2A  / craf-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics Pathway No. : 382	15.6567	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
3	PPhosphatase2A  / craf-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[1] Pathway No. : 388	15.6567	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
4	PPhosphatase2A  / craf-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[2] Pathway No. : 394	15.6567	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
5	PPhosphatase2A  / craf-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[3] Pathway No. : 400	15.6567	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
6	PPhosphatase2A  / craf-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[4] Pathway No. : 407	15.6567	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
7	PPhosphatase2A  / craf-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[5] Pathway No. : 413	15.6567	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
8	PPhosphatase2A  / craf-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[6] Pathway No. : 417	15.6567	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
9	PPhosphatase2A  / craf-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[7] Pathway No. : 425	15.6567	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
10	PPhosphatase2A  / craf-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[8] Pathway No. : 430	15.6567	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
11	PPhosphatase2A  / craf-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[9] Pathway No. : 436	15.6567	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
12	PPhosphatase2A  / craf-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[10] Pathway No. : 442	15.6567	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
13	PPhosphatase2A  / craf-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[11] Pathway No. : 448	15.6567	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
14	PPhosphatase2A  / craf-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[12] Pathway No. : 454	15.6567	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
15	PPhosphatase2A  / craf-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[13] Pathway No. : 460	15.6567	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
16	PPhosphatase2A  / craf-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[14] Pathway No. : 466	15.6567	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
17	PPhosphatase2A  / craf-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[15] Pathway No. : 472	15.6567	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
18	PPhosphatase2A  / craf-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[16] Pathway No. : 478	15.6567	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
19	PPhosphatase2A  / craf-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[17] Pathway No. : 484	15.6567	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
20	PPhosphatase2A  / craf-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[18] Pathway No. : 490	15.6567	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
21	PPhosphatase2A  / craf-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[19] Pathway No. : 496	15.6567	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
22	PPhosphatase2A  / craf-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[20] Pathway No. : 502	15.6567	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
23	PPhosphatase2A  / craf-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[21] Pathway No. : 508	15.6567	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
24	PPhosphatase2A  / craf-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[22] Pathway No. : 514	15.6567	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms
25	PPhosphatase2A  / craf-deph	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[23] Pathway No. : 520	15.6567	6	4	explicit E-S complex	Substrate craf-1* Product craf-1
	See parent PPhosphatase2A for parms

craf-1 acting as a Substrate in a reaction in  Ajay_bhalla_2007_ReacDiff1_1e-12 Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	Ras-act-unphosph -raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	Shared_Object_ Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Pathway No. : 375	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
2	Ras-act-unphosph -raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics Pathway No. : 382	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
3	Ras-act-unphosph -raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[1] Pathway No. : 388	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
4	Ras-act-unphosph -raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[2] Pathway No. : 394	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
5	Ras-act-unphosph -raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[3] Pathway No. : 400	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
6	Ras-act-unphosph -raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[4] Pathway No. : 407	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
7	Ras-act-unphosph -raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[5] Pathway No. : 413	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
8	Ras-act-unphosph -raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[6] Pathway No. : 417	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
9	Ras-act-unphosph -raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[7] Pathway No. : 425	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
10	Ras-act-unphosph -raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[8] Pathway No. : 430	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
11	Ras-act-unphosph -raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[9] Pathway No. : 436	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
12	Ras-act-unphosph -raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[10] Pathway No. : 442	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
13	Ras-act-unphosph -raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[11] Pathway No. : 448	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
14	Ras-act-unphosph -raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[12] Pathway No. : 454	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
15	Ras-act-unphosph -raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[13] Pathway No. : 460	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
16	Ras-act-unphosph -raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[14] Pathway No. : 466	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
17	Ras-act-unphosph -raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[15] Pathway No. : 472	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
18	Ras-act-unphosph -raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[16] Pathway No. : 478	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
19	Ras-act-unphosph -raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[17] Pathway No. : 484	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
20	Ras-act-unphosph -raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[18] Pathway No. : 490	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
21	Ras-act-unphosph -raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[19] Pathway No. : 496	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
22	Ras-act-unphosph -raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[20] Pathway No. : 502	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
23	Ras-act-unphosph -raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[21] Pathway No. : 508	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
24	Ras-act-unphosph -raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[22] Pathway No. : 514	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
25	Ras-act-unphosph -raf	Ajay_bhalla_ 2007_ReacDiff1_ 1e-12 Accession No. : 81	kinetics[23] Pathway No. : 520	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.