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Molecule Parameter List for GTP-Ras

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*GTP-Ras* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	25	0	0	25	75	75	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13	82	Network	Shared_Object_Ajay_Bhalla_2007_ReacDiff1_1e-13, PKC, MAPK, Ras, CaM, PKM, chain, kinetics, PKC, MAPK, Ras, CaM, PKM, kinetics[1], PKC, MAPK, Ras, CaM, PKM, kinetics[2], PKC, MAPK, Ras, CaM, PKM, kinetics[3], PKC, MAPK, Ras, CaM, PKM, kinetics[4], PKC, MAPK, Ras, CaM, PKM, kinetics[5], PKC, MAPK, Ras, kinetics[6], CaM, PKM, PKC, MAPK, Ras, CaM, PKM, kinetics[7], PKC, MAPK, Ras, CaM, PKM, kinetics[8], PKC, MAPK, Ras, CaM, PKM, kinetics[9], PKC, MAPK, Ras, CaM, PKM, kinetics[10], PKC, MAPK, Ras, CaM, PKM, kinetics[11], PKC, MAPK, Ras, CaM, PKM, kinetics[12], PKC, MAPK, Ras, CaM, PKM, kinetics[13], PKC, MAPK, Ras, CaM, PKM, kinetics[14], PKC, MAPK, Ras, CaM, PKM, kinetics[15], PKC, MAPK, Ras, CaM, PKM, kinetics[16], PKC, MAPK, Ras, CaM, PKM, kinetics[17], PKC, MAPK, Ras, CaM, PKM, kinetics[18], PKC, MAPK, Ras, CaM, PKM, kinetics[19], PKC, MAPK, Ras, CaM, PKM, kinetics[20], PKC, MAPK, Ras, CaM, PKM, kinetics[21], PKC, MAPK, Ras, CaM, PKM, kinetics[22], PKC, MAPK, Ras, CaM, PKM, kinetics[23], PKC, MAPK, Ras, CaM, PKM
This is a 25-compartment reaction-diffusion version of the Ajay_Bhalla_2007_PKM model. The original single-compartment model is repeated 25 times. In addition, a subset (27 out of 42) molecules can diffuse between compartments. Diffusion is implemented as a reaction between corresponding molecules in neighboring compartments. For D = 1e-12 m^2/sec (i.e., 1 micron^2/sec ) the kf and kb of this reaction for these 10 micron compartments are both 0.01/sec. For D = 1e-13 m^2/sec (i.e., 0.1 micron^2/sec ) the kf and kb are 0.001/sec. The stimulus file pkm_mapk22_diff_1e-13_Fig4B which was used for the model to replicate Figure 4B from the paper. pkm_mapk22_diff_1e-13_Fig4H replicate Figure 4H. pkm_mapk22_diff_1e-13_Fig4I replicate Figure 4I.

GTP-Ras acting as a Molecule in Ajay_Bhalla_2007_ReacDiff1_1e-13 Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 529	0	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 536	0	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 542	0	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 548	0	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 554	0	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 560	0	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 566	0	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 572	0	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 578	0	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 584	0	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 590	0	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 596	0	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 602	0	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 608	0	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 614	0	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 620	0	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 626	0	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 632	0	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 638	0	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 644	0	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 650	0	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 656	0	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 662	0	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 668	0	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437
GTP-Ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 674	0	1.5	No
Only a very small fraction (7% unstim, 15% stim) of ras is GTP-bound. Gibbs et al JBC 265(33) 20437

GTP-Ras acting as a Substrate for an Enzyme in Ajay_Bhalla_2007_ReacDiff1_1e-13 Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 529	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
2	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 536	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
3	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 542	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
4	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 548	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
5	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 554	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
6	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 560	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
7	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 566	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
8	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 572	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
9	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 578	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
10	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 584	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
11	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 590	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
12	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 596	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
13	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 602	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
14	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 608	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
15	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 614	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
16	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 620	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
17	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 626	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
18	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 632	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
19	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 638	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
20	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 644	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
21	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 650	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
22	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 656	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
23	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 662	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
24	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 668	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
25	GAP / GAP-inact-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 674	1.0104	10	4	explicit E-S complex	Substrate GTP-Ras Product GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.

GTP-Ras acting as a Product of an Enzyme in Ajay_Bhalla_2007_ReacDiff1_1e-13 Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 529	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

2	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 529	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
3	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 529	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
4	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 536	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

5	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 536	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
6	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 536	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
7	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 542	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

8	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 542	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
9	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 542	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
10	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 548	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

11	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 548	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
12	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 548	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
13	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 554	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

14	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 554	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
15	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 554	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
16	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 560	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

17	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 560	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
18	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 560	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
19	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 566	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

20	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 566	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
21	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 566	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
22	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 572	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

23	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 572	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
24	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 572	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
25	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 578	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

26	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 578	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
27	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 578	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
28	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 584	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

29	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 584	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
30	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 584	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
31	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 590	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

32	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 590	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
33	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 590	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
34	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 596	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

35	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 596	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
36	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 596	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
37	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 602	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

38	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 602	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
39	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 602	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
40	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 608	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

41	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 608	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
42	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 608	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
43	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 614	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

44	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 614	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
45	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 614	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
46	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 620	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

47	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 620	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
48	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 620	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
49	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 626	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

50	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 626	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
51	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 626	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
52	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 632	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

53	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 632	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
54	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 632	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
55	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 638	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

56	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 638	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
57	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 638	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
58	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 644	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

59	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 644	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
60	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 644	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
61	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 650	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

62	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 650	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
63	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 650	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
64	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 656	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

65	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 656	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
66	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 656	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
67	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 662	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

68	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 662	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
69	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 662	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
70	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 668	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

71	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 668	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
72	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 668	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
73	inact-GEF / basal_GEF_ activity	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 674	10.1015	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras

74	GEF* / GEF*-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 674	0.505057	0.02	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg-act-ras
75	CaM-GEF / CaM-GEF-act-ras	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 674	0.505057	0.2	4	explicit E-S complex	Substrate GDP-Ras Product GTP-Ras
	Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.

GTP-Ras acting as a Substrate in a reaction in Ajay_Bhalla_2007_ReacDiff1_1e-13 Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Pathway No. : 526	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
2	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 529	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
3	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Pathway No. : 526	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
4	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics Pathway No. : 533	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
5	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 536	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
6	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics Pathway No. : 533	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
7	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[1] Pathway No. : 539	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
8	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 542	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
9	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[1] Pathway No. : 539	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
10	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[2] Pathway No. : 545	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
11	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 548	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
12	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[2] Pathway No. : 545	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
13	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[3] Pathway No. : 551	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
14	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 554	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
15	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[3] Pathway No. : 551	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
16	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[4] Pathway No. : 557	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
17	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 560	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
18	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[4] Pathway No. : 557	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
19	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[5] Pathway No. : 563	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
20	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 566	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
21	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[5] Pathway No. : 563	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
22	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[6] Pathway No. : 567	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
23	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 572	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
24	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[6] Pathway No. : 567	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
25	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[7] Pathway No. : 575	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
26	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 578	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
27	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[7] Pathway No. : 575	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
28	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[8] Pathway No. : 581	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
29	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 584	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
30	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[8] Pathway No. : 581	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
31	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[9] Pathway No. : 587	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
32	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 590	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
33	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[9] Pathway No. : 587	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
34	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[10] Pathway No. : 593	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
35	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 596	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
36	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[10] Pathway No. : 593	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
37	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[11] Pathway No. : 599	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
38	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 602	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
39	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[11] Pathway No. : 599	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
40	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[12] Pathway No. : 605	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
41	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 608	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
42	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[12] Pathway No. : 605	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
43	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[13] Pathway No. : 611	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
44	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 614	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
45	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[13] Pathway No. : 611	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
46	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[14] Pathway No. : 617	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
47	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 620	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
48	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[14] Pathway No. : 617	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
49	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[15] Pathway No. : 623	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
50	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 626	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
51	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[15] Pathway No. : 623	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
52	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[16] Pathway No. : 629	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
53	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 632	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
54	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[16] Pathway No. : 629	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
55	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[17] Pathway No. : 635	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
56	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 638	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
57	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[17] Pathway No. : 635	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
58	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[18] Pathway No. : 641	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
59	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 644	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
60	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[18] Pathway No. : 641	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
61	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[19] Pathway No. : 647	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
62	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 650	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
63	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[19] Pathway No. : 647	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
64	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[20] Pathway No. : 653	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
65	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 656	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
66	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[20] Pathway No. : 653	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
67	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[21] Pathway No. : 659	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
68	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 662	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
69	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[21] Pathway No. : 659	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
70	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[22] Pathway No. : 665	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
71	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 668	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
72	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[22] Pathway No. : 665	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.
73	Ras-act-craf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[23] Pathway No. : 671	9.9998 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.05(uM)	-	Substrate GTP-Ras craf-1* Product Raf*-GTP-Ras
	Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
74	Ras-intrinsic-GT Pase	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Ras Pathway No. : 674	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras Product GDP-Ras
	This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
75	Ras-act-unphosph -raf	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[23] Pathway No. : 671	0 (uM^-1 s^-1)	0 (s^-1)	-	-	Substrate GTP-Ras craf-1 Product Raf-GTP-Ras
	18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.

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