| |
Reaction
Name | Pathway Name /
Pathway No. | Kf | Kb | Kd | tau | Reagents |
| 1 | CaM_bind_PDE1 | AC
Pathway No. 362 | 720
(uM^-1 s^-1) | 5
(s^-1) | Kd(bf) = 0.0069(uM) | - | Substrate:
PDE1
CaM-Ca4
Products:
CaM.PDE1
|
| For olf epi PDE1, affinity is 7 nM. Assume same for brain. Reaction should be pretty fast. Assume kb = 5/sec. Then kf = 5 / (0.007 * 6e5) = 1.2e-3 | | 2 | CaM-bind-AC1 | AC
Pathway No. 362 | 49.9995
(uM^-1 s^-1) | 1
(s^-1) | Kd(bf) = 0.02(uM) | - | Substrate:
CaM-Ca4
AC1
Products:
AC1-CaM
|
| Half-max at 20 nM CaM (Tang et al JBC 266:13 8595-8603 1991 kb/kf = 20 nM = 12000 #/cell so kf = kb/12000 = kb * 8.333e-5 | | 3 | Gs-bind-AC2 | AC
Pathway No. 362 | 499.995
(uM^-1 s^-1) | 1
(s^-1) | Kd(bf) = 0.002(uM) | - | Substrate:
AC2
Gs-alpha
Products:
AC2-Gs
|
| Half-max at around 3nM = kb/kf from fig 5 in Feinstein et al PNAS USA 88 10173-10177 1991 kf = kb/1800 = 5.56e-4 kb Ofer's thesis data indicates it is more like 2 nM. kf = kb/1200 = 8.33e-4 | | 4 | Gs-bind-AC1 | AC
Pathway No. 362 | 126
(uM^-1 s^-1) | 1
(s^-1) | Kd(bf) = 0.0079(uM) | - | Substrate:
Gs-alpha
AC1
Products:
AC1-Gs
|
| Half-max 8nM from Tang et al JBC266:13 8595-8603 kb/kf = 8 nM = 4800#/cell so kf = kb * 2.08e-4 | | 5 | Gs-bind-AC2* | AC
Pathway No. 362 | 833.283
(uM^-1 s^-1) | 1
(s^-1) | Kd(bf) = 0.0012(uM) | - | Substrate:
Gs-alpha
AC2*
Products:
AC2*-Gs
|
| kb/kf = 1.2 nM so kf = kb/720 = 1.3888 * kb. | | 6 | dephosph-AC2 | AC
Pathway No. 362 | 0.1
(s^-1) | 0
(s^-1) | - | - | Substrate:
AC2*
Products:
AC2
|
| Random rate. | | 7 | dephosph-PDE | AC
Pathway No. 362 | 0.1
(s^-1) | 0
(s^-1) | - | - | Substrate:
cAMP-PDE*
Products:
cAMP-PDE
|
| The rates for this are poorly constrained. In adipocytes (probably a different PDE) the dephosphorylation is complete within 15 min, but there are no intermediate time points so it could be much faster. Identity of phosphatase etc is still unknown. |
and 
color.
