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Molecule List for pathway PLA2 (Pathway Number 183) in Accession MAPK-bistability-fig1c (Accession Number 35) | Default ordering is done according to Pathway Number. Table headers can be used for changing the default ordering.
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The entries are grouped according to Pathway Number and are alternately color coded using  and  color. |
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Name | Accession
Type | Initial
Conc.
(uM) | Volume
(fL) | Buffered | Sum Total Of | | 1 | PLA2-cytosolic | Network | 0.4 | 0 | No | - | | | cPLA2 IV form has mol wt of 85 Kd. Glaser et al 1993 TIPS 14:92-98. Calculated cytosolic concentration is ~300 nM from Wijkander and Sundler 1991 Eur J Biochem 202:873 Leslie and Channon 1990 BBA 1045:261 use about 400 nM. Decent match. Use 400 nM. | | 2 | PLA2-Ca* | Network | 0 | 0 | No | - | | | The generic Ca-activated form ofPLA2. Leslie and Channon 1990 BBA 1045:261. | | 3 | PIP2-PLA2* | Network | 0 | 0 | No | - | | 4 | PIP2-Ca-PLA2* | Network | 0 | 0 | No | - | | 5 | DAG-Ca-PLA2* | Network | 0 | 0 | No | - | | 6 | PLA2*-Ca | Network | 0 | 0 | No | - | | | Phosphorylated and active form of PLA2. Several kinases act on it: PKA: Wightman et al JBC 257 pp6650 1982 PKC: Many refs, eg Gronich et al JBC 263 pp 16645, 1988 but see Lin etal MAPK: Lin et al, Cell 72 pp 269, 1993. Show 3x with MAPK but not PKC alone The Nemenoff assays are conducted in rather high Ca so I have assumed a Ca binding step. | | 7 | PLA2* | Network | 0 | 0 | No | - | | | Phosphorylated PLA2. The site differs from the site phosphorylated by PKC. See Nemenoff et al 1993 JBC 268(3):1960-1964 | | 8 | APC | Network | 30 | 0 | Yes | - | | | arachodonylphosphatidylcholine is the favoured substrate from Wijkander and Sundler, JBC 202 pp 873-880, 1991. Their assay used 30 uM substrate, which is what the kinetics in this model are based on. For the later model we should locate a more realistic value for APC. For now it is treated as a buffered metabolite. |
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