| | Molecule Name/
Site Name | Km (uM) | kcat (1/s) | Ratio
(k2/k3) | Enzyme Type | Substrate | Product |
| 1 |
Enzyme Activity:
1
Enzyme Molecule:
Ras-MKKKK | 0.01 | 2.5 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | MKKK
| MKKK-P
|
| | The Km is 10nM and Vmax is 2.5nM sec^-1. We assume that there is 1 nM of the Ras-MKKKK. From Kholodenko, 2000. If the enzymes are not flagged as Available, then this Km should be set to 0.1 to obtain oscillations. |
| 2 |
Enzyme Activity:
10
Enzyme Molecule:
int5 | 0.0149997 | 0.5 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | MAPK-P
| MAPK
|
| | The Km is 15nM and Vmax is 0.5nM.s-1 from Kholodenko, 2000 |
| 3 |
Enzyme Activity:
2
Enzyme Molecule:
int1 | 0.008 | 0.25 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | MKKK-P
| MKKK
|
| | Km is 8nM and Vmax is 0.25nM.s-1 from Kholodenko, 2000. |
| 4 |
Enzyme Activity:
3
Enzyme Molecule:
MKKK-P | 0.0150001 | 0.025 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | MKK
| MKK-P
|
| | Km is 15 nM and Vmax is 0.025s-1 from Kholodenko, 2000 |
| 5 |
Enzyme Activity:
4
Enzyme Molecule:
MKKK-P | 0.0150001 | 0.025 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | MKK-P
| MKK-PP
|
| | Km is 15nM and Vmax is 0.025s-1 from Kholodenko, 2000. |
| 6 |
Enzyme Activity:
5
Enzyme Molecule:
int2 | 0.015 | 0.75 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | MKK-PP
| MKK-P
|
| | The Km is 15nM and Vmax is 0.75nM.s-1 from Kholodenko, 2000 |
| 7 |
Enzyme Activity:
6
Enzyme Molecule:
int3 | 0.015 | 0.75 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | MKK-P
| MKK
|
| | The Km is 15nM and the Vmax is 0.75nM.s-1 from Kholodenko 2000. |
| 8 |
Enzyme Activity:
7
Enzyme Molecule:
MKK-PP | 0.0150001 | 0.025 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | MAPK
| MAPK-P
|
| | The Km is 15nM which is 0.015uM Vmax is 0.025s-1 from Kholodenko, 2000. |
| 9 |
Enzyme Activity:
8
Enzyme Molecule:
MKK-PP | 0.0150001 | 0.025 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | MAPK-P
| MAPK-PP
|
| | The Km is 15nM which is 0.015uM and Vmax is 0.025s-1 from Kholodenko, 2000 |
| 10 |
Enzyme Activity:
9
Enzyme Molecule:
int4 | 0.0149997 | 0.5 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | MAPK-PP
| MAPK-P
|
| | The Km is 15nM and Vmax is 0.5nM.s-1 from Kholodenko, 2000 |
