| | Name | Initial Conc. (uM) | Volume (fL) | Buffered |
| 1 | NF1 | 9.9994 | 523.6 | No |
| | Neurofibromin a mammalian GAP NF1 conc = 10 um pp.3959 Refer: Phillips RA et al 2003 Biochemistry 42: 3956-3965 |
| 2 | Pi | 0 | 523.6 | No |
| | Inorganic Phosphate is released (Pi) |
| 3 | RasGDP | 0 | 523.6 | No |
| | Inactive Ras GDP is formed. |
| 4 | RasGDP-NF1_Pi | 0 | 523.6 | No |
| | NF1 bound to N-ras complexed to GDP.Pi NF1 is a GAP Phillips RA et al 2003 Biochemistry 42: 3956-3965 |
| 5 | RasGDP_NF1 | 0 | 523.6 | No |
| | NF1 bound to N-ras complexed to GDP NF1 is a mammalian GAP Phillips RA et al 2003 Biochemistry 42: 3956-3965 |
| 6 | RasGTP | 1 | 523.6 | No |
| | The concentration of Ras GTP is 1 um. Ref: Fig-2 Phillips RA et al Biochemistry 2003, 42, 3956-3965. |
| 7 | RasGTP-NF1 | 0 | 523.6 | No |
| | NF1 bound to N-ras complexed to GTP NF1 is a mammalian GAP Phillips RA et al 2003 Biochemistry 42: 3956-3965 |
| 8 | RasGTP-NF1* | 0 | 523.6 | No |
| | NF1 bound to activated N-ras complexed to GTP NF1 is a mammalian GAP Phillips RA et al 2003 Biochemistry 42: 3956-3965 |
