| | Name | Initial Conc. (uM) | Volume (fL) | Buffered |
| 1 | Im-nNOS | 1 | 0.0016667 | No |
| | Imidazole bound rat brain neuronal Nitric Oxide Synthase.Abu-Soud HM et al. Biochemistry. 1999 Sep 21;38(38):12446-51. |
| 2 | Im-nNOS-L-Arginine | 0 | 0.0016667 | No |
| | An intermediate formed due to rapid binding of L-Arginine to Imidazole bound neuronal Nitric Oxide Synthase. Abu-Soud HM et al. Biochemistry. 1999 Sep 21;38(38):12446-51. |
| 3 | Imidazole | 0 | 0.0016667 | No |
| | Imidazole released from the neuronal Nitric Oxide Synthase complexed to L-Arginine. Abu-Soud HM et al. Biochemistry. 1999 Sep 21;38(38):12446-51. |
| 4 | L-Arginine | 6000 | 0.0016667 | No |
| | L-Arginine, a normal substrate for Nitric Oxide Synthase Abu-Soud HM et al. Biochemistry. 1999 Sep 21;38(38):12446-51. |
| 5 | nNOS-L-Arginine | 0 | 0.0016667 | No |
| | L-Arginine bound to neuronal Nitric Oxide Synthase. Abu-Soud HM et al. Biochemistry. 1999 Sep 21;38(38):12446-51. |
