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Molecule Parameter List for Im-nNOS-L-Arginine

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
Im-nNOS-L-Arginine participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000011

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
L-Arginine72Pathway
L-Arginine 
This model was taken from the Abu-Soud HM et al. Biochemistry. 1999 Sep 21;38(38):12446-51. This model shows kinetic binding of L-Arginine to neuronal nitric oxide synthase.
Model shows the substrate (L-Arginine) binding to nNOS in a two-step reversible fashion. First there is rapid binding equilibrium between Im-nNOS and L-Arginine to form an intermediate that contains bound imidazole and L-Arginine. This is followed by a slower conformational change in the Im-enzyme-substrate complex that is associated with release of bound imidazole and generation of a modified enzyme-substrate complex which is detected due to spectral change. Replicates Figure 4 (right panel).

Im-nNOS-L-Arginine acting as a Molecule in  
L-Arginine Network
NameAccession NamePathway NameInitial Conc.
(uM)
Volume
(fL)
Buffered
Im-nNOS-L-ArginineL-Arginine
Accession No. : 72
L-Arginine
Pathway No. : 308
00.0016667No
An intermediate formed due to rapid binding of L-Arginine to Imidazole bound neuronal Nitric Oxide Synthase. Abu-Soud HM et al. Biochemistry. 1999 Sep 21;38(38):12446-51.

Im-nNOS-L-Arginine acting as a Substrate in a reaction in  
L-Arginine Network
Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
NameAccession NamePathway NameKfKbKdtauReagents
  • Imidazole_
    release
  • L-Arginine
    Accession No. : 72
    L-Arginine
    Pathway No. : 308
    81
    (s^-1)
    0.45
    (uM^-1 s^-1)
    Kd(cb) = 0.0056(uM)-Substrate
  • Im-nNOS-L-Argini
    ne


    Product
    Imidazole
    nNOS-L-Arginine
  • Release of Imidazole from complex of L-Arginine and Imidazole bound rat brain neuronal Nitric Oxide Synthase. Abu-Soud HM et al. Biochemistry. 1999 Sep 21;38(38):12446-51.

    Im-nNOS-L-Arginine acting as a Product in a reaction in  
    L-Arginine Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
    NameAccession NamePathway NameKfKbKdtauReagents
  • Arginine_
    binding
  • L-Arginine
    Accession No. : 72
    L-Arginine
    Pathway No. : 308
    250
    (uM^-1 s^-1)
    125000
    (s^-1)
    Kd(bf) = 500(uM)-Substrate
    Im-nNOS
    L-Arginine

    Product
  • Im-nNOS-L-Argini
    ne

  • Binding of L-Arginine to Imidazole bound rat brain neuronal Nitric Oxide Synthase. Kd = 0.5mM = 500uM. Abu-Soud HM et al. Biochemistry. 1999 Sep 21;38(38):12446-51.



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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