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Reaction List for pathway RasGTPase (Pathway Number 307) |
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reactions is not considered. |
| Name | Kf | Kb | Kd | tau | Substrate | Product | |
| 1 | GAP_ dissociation | 46.5 (s^-1) | 1.2 (uM^-1 s^-1) | Kd(cb) = 0.0258(uM) | - | RasGDP_NF1 | RasGDP NF1 |
| Dissociation of NF1 from Ras.GDP NF1 is a mammalian GAP Kf = 46.5 /sec Kb = 1.2 /sec/uM Table 3, Phillips RA et al 2003 Biochemistry 42: 3956-3965 | |||||||
| 2 | GTP_ hydrolysis[1] | 19.5 (s^-1) | 0.22 (s^-1) | Keq = 0.0113(uM) | 0.051sec | RasGTP-NF1* | RasGDP-NF1_Pi |
| First step in hydrolysis of GTP bound to Ras complexed with NF1 - a mammalian GAP Kf = 19.5 /sec Kb = 0.22 /sec Table 3, Phillips RA et al 2003 Biochemistry 42: 3956-3965 | |||||||
| 3 | GTP_ hydrolysis[2] | 40 (s^-1) | 0.0001 (uM^-1 s^-1) | Kd(cb) = 0(uM) | - | RasGDP-NF1_Pi | Pi RasGDP_NF1 |
| Second step in hydrolysis of GTP bound to Ras is complexed with NF1 - a mammalian GAP Kf = 40 /sec Kb = 108 /M/sec = 1.08e-04 /uM/sec Phillips RA et al 2003 Biochemistry 42: 3956-3965 | |||||||
| 4 | NF1_binding | 1.2 (uM^-1 s^-1) | 6.36 (s^-1) | Kd(bf) = 5.3(uM) | - | RasGTP NF1 | RasGTP-NF1 |
| Binding of NF1 to Ras.GTP NF1 is a mammalian GAP Kd = 5.3 uM Table 3, Phillips RA et al 2003 Biochemistry 42: 3956-3965 | |||||||
| 5 | Ras_activation | 418 (s^-1) | 5.5 (s^-1) | Keq = 0.0132(uM) | 0.002sec | RasGTP-NF1 | RasGTP-NF1* |
| Activation of Ras by GAP (i.e NF1) Kf = 418 /sec Kb = 5.5 /sec Table 3, Phillips RA et al 2003 Biochemistry 42: 3956-3965 | |||||||
| Pathway Detail | Molecule List | Enzyme List | Reaction List |