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Reaction Name | Pathway Name / Pathway No.![](database/fig/up.png) | Kf | Kb | Kd | tau | Reagents |
1 | dephosph-GEF | Ras
Pathway No. 1037 | 1 (s^-1) | 0 (s^-1) | - | - | Substrate: GEF*
Products: inact-GEF
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2 | Ras-intrinsic-GT Pase | Ras
Pathway No. 1037 | 0.0001 (s^-1) | 0 (s^-1) | - | - | Substrate: GTP-Ras
Products: GDP-Ras
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| This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4 | 3 | dephosph-GAP | Ras
Pathway No. 1037 | 0.1 (s^-1) | 0 (s^-1) | - | - | Substrate: GAP*
Products: GAP
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| Assume a reasonably good rate for dephosphorylating it, 1/sec | 4 | CaM-bind-GEF | Ras
Pathway No. 1037 | 199.995 (uM^-1 s^-1) | 1 (s^-1) | Kd(bf) = 0.005(uM) | - | Substrate: inact-GEF CaM-Ca4
Products: CaM-GEF
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| We have no numbers for this. It is probably between the two extremes represented by the CaMKII phosph states, and I have used guesses based on this. kf=1e-4 kb=1 The reaction is based on Farnsworth et al Nature 376 524-527 1995 28 Feb 2006: Increased affinity 36-fold to account for Ca input to MAPK cascade, possibly folding in other pathway inputs. 21 April 2006: Altered affinity to same level as pkm_mapk21.g model to prevent spontaneous turnon. Kf = 200, Kb = 1. |