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Molecule Parameter List for Raf*-GTP-Ras

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
Raf*-GTP-Ras participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences2505000025

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Ajay_Bhalla_
    2007_ReacDiff3
  • 84NetworkShared_Object_Ajay_Bhalla_2007_ReacDiff3 PKC PLA2 
    MAPK PLA2 Ras CaM chain kinetics PKC MAPK Ras CaM kinetics[1] 
    PKC PLA2 MAPK Ras CaM kinetics[2] PKC PLA2 MAPK Ras CaM kinetics[3] 
    PKC PLA2 MAPK Ras CaM kinetics[4] PKC PLA2 MAPK Ras CaM kinetics[5] 
    PKC PLA2 MAPK Ras MAPK CaM kinetics[6] PKC PLA2 MAPK Ras 
    CaM kinetics[7] PKC PLA2 MAPK Ras CaM PKC kinetics[8] PLA2 
    MAPK Ras CaM kinetics[9] PKC PLA2 MAPK Ras CaM kinetics[10] 
    PKC PLA2 MAPK Ras CaM kinetics[11] PKC PLA2 MAPK Ras CaM 
    kinetics[12] PKC PLA2 Ras CaM kinetics[13] PKC PLA2 MAPK 
    Ras CaM kinetics[14] PKC PLA2 MAPK Ras CaM kinetics[15] 
    PKC PLA2 MAPK Ras kinetics[16] CaM PKC PLA2 MAPK Ras CaM 
    kinetics[17] PKC PLA2 MAPK Ras CaM kinetics[18] PKC PLA2 
    MAPK Ras CaM kinetics[19] PKC PLA2 MAPK Ras CaM kinetics[20] 
    PKC PLA2 MAPK Ras CaM kinetics[21] PKC PLA2 MAPK Ras CaM 
    kinetics[22] PKC PLA2 MAPK Ras CaM kinetics[23] PKC PLA2 
    MAPK Ras CaM 
    This is a 25-compartment reaction-diffusion version of the Ajay_Bhalla_2007_bistable model. The original single-compartment model is repeated 25 times.
    In addition, a subset (33 out of 50) molecules can diffuse between compartments. Diffusion is implemented as a reaction between corresponding molecules in neighboring compartments. Here D = 1e-13 m^2/sec (i.e., 0.1 micron^2/sec ) so the kf and kb of this reaction for these 10 micron compartments are both 0.001/sec.
    The basal calcium level in this model is held at 95 nM which is rather close to threshold for the flip to the active state. This is necessary to sustain active propagation of activation.
    The stimulus file bis6-propgn_D1e-13_FigEF which was used for the model to replicate Figure 4E and 4F from the paper.

    Raf*-GTP-Ras acting as a Molecule in  
    Ajay_Bhalla_2007_ReacDiff3 Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    Raf*-GTP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 921
    0125.7No
    Raf*-GTP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 928
    0125.7No
    Raf*-GTP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 934
    0125.7No
    Raf*-GTP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 940
    0125.7No
    Raf*-GTP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 946
    0125.7No
    Raf*-GTP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 952
    0125.7No
    Raf*-GTP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 958
    0125.7No
    Raf*-GTP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 965
    0125.7No
    Raf*-GTP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 971
    0125.7No
    Raf*-GTP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 977
    0125.7No
    Raf*-GTP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 983
    0125.7No
    Raf*-GTP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 989
    0125.7No
    Raf*-GTP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 995
    0125.7No
    Raf*-GTP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 960
    0125.7No
    Raf*-GTP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1006
    0125.7No
    Raf*-GTP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1012
    0125.7No
    Raf*-GTP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1018
    0125.7No
    Raf*-GTP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1024
    0125.7No
    Raf*-GTP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1030
    0125.7No
    Raf*-GTP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1036
    0125.7No
    Raf*-GTP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1042
    0125.7No
    Raf*-GTP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1048
    0125.7No
    Raf*-GTP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1054
    0125.7No
    Raf*-GTP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1060
    0125.7No
    Raf*-GTP-Ras
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1066
    0125.7No

    Raf*-GTP-Ras acting as an Enzyme in  
    Ajay_Bhalla_2007_ReacDiff3 Network
     Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    1Raf*-GTP-Ras /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 921
    0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    2Raf*-GTP-Ras /
    Raf*-GTP-Ras.2
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 921
    0.1590960.34explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
    3Raf*-GTP-Ras /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 928
    0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    4Raf*-GTP-Ras /
    Raf*-GTP-Ras.2
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 928
    0.1590960.34explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
    5Raf*-GTP-Ras /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 934
    0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    6Raf*-GTP-Ras /
    Raf*-GTP-Ras.2
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 934
    0.1590960.34explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
    7Raf*-GTP-Ras /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 940
    0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    8Raf*-GTP-Ras /
    Raf*-GTP-Ras.2
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 940
    0.1590960.34explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
    9Raf*-GTP-Ras /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 946
    0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    10Raf*-GTP-Ras /
    Raf*-GTP-Ras.2
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 946
    0.1590960.34explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
    11Raf*-GTP-Ras /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 952
    0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    12Raf*-GTP-Ras /
    Raf*-GTP-Ras.2
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 952
    0.1590960.34explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
    13Raf*-GTP-Ras /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 958
    0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    14Raf*-GTP-Ras /
    Raf*-GTP-Ras.2
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 958
    0.1590960.34explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
    15Raf*-GTP-Ras /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 965
    0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    16Raf*-GTP-Ras /
    Raf*-GTP-Ras.2
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 965
    0.1590960.34explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
    17Raf*-GTP-Ras /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 971
    0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    18Raf*-GTP-Ras /
    Raf*-GTP-Ras.2
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 971
    0.1590960.34explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
    19Raf*-GTP-Ras /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 977
    0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    20Raf*-GTP-Ras /
    Raf*-GTP-Ras.2
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 977
    0.1590960.34explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
    21Raf*-GTP-Ras /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 983
    0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    22Raf*-GTP-Ras /
    Raf*-GTP-Ras.2
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 983
    0.1590960.34explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
    23Raf*-GTP-Ras /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 989
    0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    24Raf*-GTP-Ras /
    Raf*-GTP-Ras.2
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 989
    0.1590960.34explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
    25Raf*-GTP-Ras /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 995
    0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    26Raf*-GTP-Ras /
    Raf*-GTP-Ras.2
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 995
    0.1590960.34explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
    27Raf*-GTP-Ras /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 960
    0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    28Raf*-GTP-Ras /
    Raf*-GTP-Ras.2
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 960
    0.1590960.34explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
    29Raf*-GTP-Ras /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1006
    0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    30Raf*-GTP-Ras /
    Raf*-GTP-Ras.2
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1006
    0.1590960.34explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
    31Raf*-GTP-Ras /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1012
    0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    32Raf*-GTP-Ras /
    Raf*-GTP-Ras.2
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1012
    0.1590960.34explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
    33Raf*-GTP-Ras /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1018
    0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    34Raf*-GTP-Ras /
    Raf*-GTP-Ras.2
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1018
    0.1590960.34explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
    35Raf*-GTP-Ras /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1024
    0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    36Raf*-GTP-Ras /
    Raf*-GTP-Ras.2
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1024
    0.1590960.34explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
    37Raf*-GTP-Ras /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1030
    0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    38Raf*-GTP-Ras /
    Raf*-GTP-Ras.2
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1030
    0.1590960.34explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
    39Raf*-GTP-Ras /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1036
    0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    40Raf*-GTP-Ras /
    Raf*-GTP-Ras.2
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1036
    0.1590960.34explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
    41Raf*-GTP-Ras /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1042
    0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    42Raf*-GTP-Ras /
    Raf*-GTP-Ras.2
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1042
    0.1590960.34explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
    43Raf*-GTP-Ras /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1048
    0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    44Raf*-GTP-Ras /
    Raf*-GTP-Ras.2
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1048
    0.1590960.34explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
    45Raf*-GTP-Ras /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1054
    0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    46Raf*-GTP-Ras /
    Raf*-GTP-Ras.2
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1054
    0.1590960.34explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
    47Raf*-GTP-Ras /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1060
    0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    48Raf*-GTP-Ras /
    Raf*-GTP-Ras.2
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1060
    0.1590960.34explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
    49Raf*-GTP-Ras /
    Raf*-GTP-Ras.1
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1066
    0.1590960.34explicit E-S complexSubstrate
    MAPKK

    Product
    MAPKK-ser
        Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
    50Raf*-GTP-Ras /
    Raf*-GTP-Ras.2
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • MAPK
    Pathway No. : 1066
    0.1590960.34explicit E-S complexSubstrate
    MAPKK-ser

    Product
    MAPKK*
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6

    Raf*-GTP-Ras acting as a Product in a reaction in  
    Ajay_Bhalla_2007_ReacDiff3 Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1Ras-act-craf
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • Shared_Object_
    Ajay_Bhalla_
    2007_ReacDiff3

    Pathway No. : 918
  • 9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    2Ras-act-craf
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics
    Pathway No. : 926
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    3Ras-act-craf
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[1]
    Pathway No. : 931
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    4Ras-act-craf
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[2]
    Pathway No. : 937
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    5Ras-act-craf
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[3]
    Pathway No. : 943
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    6Ras-act-craf
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[4]
    Pathway No. : 949
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    7Ras-act-craf
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[5]
    Pathway No. : 955
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    8Ras-act-craf
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[6]
    Pathway No. : 962
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    9Ras-act-craf
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[7]
    Pathway No. : 968
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    10Ras-act-craf
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[8]
    Pathway No. : 975
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    11Ras-act-craf
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[9]
    Pathway No. : 980
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    12Ras-act-craf
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[10]
    Pathway No. : 986
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    13Ras-act-craf
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[11]
    Pathway No. : 992
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    14Ras-act-craf
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[12]
    Pathway No. : 998
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    15Ras-act-craf
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[13]
    Pathway No. : 1003
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    16Ras-act-craf
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[14]
    Pathway No. : 1009
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    17Ras-act-craf
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[15]
    Pathway No. : 1015
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    18Ras-act-craf
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[16]
    Pathway No. : 1020
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    19Ras-act-craf
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[17]
    Pathway No. : 1027
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    20Ras-act-craf
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[18]
    Pathway No. : 1033
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    21Ras-act-craf
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[19]
    Pathway No. : 1039
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    22Ras-act-craf
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[20]
    Pathway No. : 1045
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    23Ras-act-craf
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[21]
    Pathway No. : 1051
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    24Ras-act-craf
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[22]
    Pathway No. : 1057
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.
    25Ras-act-craf
  • Ajay_Bhalla_
    2007_ReacDiff3

    Accession No. : 84
  • kinetics[23]
    Pathway No. : 1063
    9.9998
    (uM^-1 s^-1)
    0.5
    (s^-1)
    Kd(bf) = 0.05(uM)-Substrate
    GTP-Ras
    craf-1*

    Product
    Raf*-GTP-Ras
      Assume the binding is fast and limited only by the amount of Ras* available. So kf=kb/[craf-1] If kb is 1/sec, then kf = 1/0.2 uM = 1/(0.2 * 6e5) = 8.3e-6 Later: Raise it by 10 X to 4e-5 From Hallberg et al JBC 269:6 3913-3916 1994, 3% of cellular Raf is complexed with Ras. So we raise kb 4x to 4 This step needed to memb-anchor and activate Raf: Leevers et al Nature 369 411-414 May 16, 2003 Changed Ras and Raf to synaptic levels, an increase of about 2x for each. To maintain the percentage of complexed Raf, reduced the kf by 2.4 fold to 10.



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